LOCUS       BBa_K185011             1453 bp    DNA     linear       18-Nov-2024
DEFINITION  Promoter116+QPI+RBS31+RelB+Double-terminator
ACCESSION   BBa_K185011
VERSION     BBa_K185011.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23116
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             65..772
                     /label=cI lambda
     misc_feature    773..805
                     /label=LVA
     misc_feature    812..836
                     /label=Help:Barcodes
     CDS             62..811
                     /label=BBa_C0051
     stem_loop       856..899
                     /label=stem_loop
     misc_feature    845..924
                     /label=BBa_B0010
     terminator      845..924
                     /label=BBa_B0010
     stem_loop       940..959
                     /label=T7 TE
     misc_feature    966..966
                     /label=stop
     polyA_site      960..973
                     /label=polya
     misc_feature    933..973
                     /label=BBa_B0012
     terminator      933..973
                     /label=BBa_B0012
     promoter        1019..1024
                     /label=-10
     promoter        996..1001
                     /label=-35
     misc_feature    1006..1022
                     /label=OR1
     misc_feature    982..998
                     /label=OR2
     misc_feature    982..1030
                     /label=BBa_R0051
     promoter        982..1030
                     /label=BBa_R0051
     misc_feature    44..1030
                     /label=BBa_Q04510
     misc_feature    1045..1048
                     /label=conserved
     RBS             1039..1052
                     /label=BBa_B0031
     misc_feature    1059..1061
                     /label=start
     CDS             1059..1298
                     /label=cds
     misc_feature    1314..1316
                     /label=stop
     misc_feature    1299..1313
                     /label=His tag
     CDS             1059..1316
                     /label=BBa_K185048
     stem_loop       1336..1379
                     /label=stem_loop
     misc_feature    1325..1404
                     /label=BBa_B0010
     terminator      1325..1404
                     /label=BBa_B0010
     stem_loop       1420..1439
                     /label=T7 TE
     misc_feature    1446..1446
                     /label=stop
     polyA_site      1440..1453
                     /label=polya
     misc_feature    1413..1453
                     /label=BBa_B0012
     terminator      1413..1453
                     /label=BBa_B0012
ORIGIN
        1 ttgacagcta gctcagtcct agggactatg ctagctacta gagaaagagg agaaatacta
       61 gatgagcaca aaaaagaaac cattaacaca agagcagctt gaggacgcac gtcgccttaa
      121 agcaatttat gaaaaaaaga aaaatgaact tggcttatcc caggaatctg tcgcagacaa
      181 gatggggatg gggcagtcag gcgttggtgc tttatttaat ggcatcaatg cattaaatgc
      241 ttataacgcc gcattgcttg caaaaattct caaagttagc gttgaagaat ttagcccttc
      301 aatcgccaga gaaatctacg agatgtatga agcggttagt atgcagccgt cacttagaag
      361 tgagtatgag taccctgttt tttctcatgt tcaggcaggg atgttctcac ctgagcttag
      421 aacctttacc aaaggtgatg cggagagatg ggtaagcaca accaaaaaag ccagtgattc
      481 tgcattctgg cttgaggttg aaggtaattc catgaccgca ccaacaggct ccaagccaag
      541 ctttcctgac ggaatgttaa ttctcgttga ccctgagcag gctgttgagc caggtgattt
      601 ctgcatagcc agacttgggg gtgatgagtt taccttcaag aaactgatca gggatagcgg
      661 tcaggtgttt ttacaaccac taaacccaca gtacccaatg atcccatgca atgagagttg
      721 ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa gagacgtttg gcgctgcaaa
      781 cgacgaaaac tacgctttag tagcttaata acgctgatag tgctagtgta gatcgctact
      841 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
      901 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
      961 ttctgcgttt atatactaga gtaacaccgt gcgtgttgac tattttacct ctggcggtga
     1021 taatggttgc tactagagtc acacaggaaa cctactagat gggtagcatt aacctgcgta
     1081 ttgacgatga acttaaagcg cgttcttacg ccgcgcttga aaaaatgggt gtaactcctt
     1141 ctgaagcgct tcgtctcatg ctcgagtata tcgctgacaa tgaacgcttg ccgttcaaac
     1201 agacactcct gagtgatgaa gatgctgaac ttgtggagat agtgaaagaa cggcttcgta
     1261 atcctaagcc agtacgtgtg acgctggatg aactccatca tcatcatcat cactgatact
     1321 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     1381 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     1441 ttctgcgttt ata
//