LOCUS BBa_K185033 1514 bp DNA linear 22-Dec-2024 DEFINITION An inverter of a special lactose operon system based on J23110-rbs34-lacI-dter-plac-rbs31 ACCESSION BBa_K185033 VERSION BBa_K185033.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23110 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 CDS 62..1147 /label=BBa_I732100 stem_loop 1167..1210 /label=stem_loop misc_feature 1156..1235 /label=BBa_B0010 terminator 1156..1235 /label=BBa_B0010 stem_loop 1251..1270 /label=T7 TE misc_feature 1277..1277 /label=stop polyA_site 1271..1284 /label=polya misc_feature 1244..1284 /label=BBa_B0012 terminator 1244..1284 /label=BBa_B0012 CDS 1293..1380 /label=end of LacI coding region (inactive) misc_feature 1293..1492 /label=BBa_R0010 misc_feature 1381..1418 /label=CAP binding site promoter 1429..1434 /label=-35 promoter 1453..1458 /label=-10 misc_feature 1458..1492 /label=LacI binding site misc_feature 1465..1465 /label=start promoter 1293..1492 /label=BBa_R0010 misc_feature 1507..1510 /label=conserved RBS 1501..1514 /label=BBa_B0031 ORIGIN 1 tttacggcta gctcagtcct aggtacaatg ctagctacta gagaaagagg agaaatacta 61 gatgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt 121 ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc 181 ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca 241 gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt 301 cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga 361 acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag 421 tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg 481 cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat 541 tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca 601 gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg 661 ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg 721 gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac 781 tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc 841 cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc 901 atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag 961 cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc 1021 cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg 1081 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca 1141 gtaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct 1201 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt 1261 cgggtgggcc tttctgcgtt tatatactag agcaatacgc aaaccgcctc tccccgcgcg 1321 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga 1381 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat 1441 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca catactagag 1501 tcacacagga aacc //