LOCUS       BBa_K185050             2492 bp    DNA     linear       26-Nov-2024
DEFINITION  Mutated Lon protease + double terminator
ACCESSION   BBa_K185050
VERSION     BBa_K185050.1  
FEATURES             Location/Qualifiers
     misc_feature    1..2355
                     /label=BBa_K185055
     CDS             1..2355
                     /label=BBa_K185055
     stem_loop       2375..2418
                     /label=stem_loop
     misc_feature    2364..2443
                     /label=BBa_B0010
     terminator      2364..2443
                     /label=BBa_B0010
     stem_loop       2459..2478
                     /label=T7 TE
     misc_feature    2485..2485
                     /label=stop
     polyA_site      2479..2492
                     /label=polya
     misc_feature    2452..2492
                     /label=BBa_B0012
     terminator      2452..2492
                     /label=BBa_B0012
ORIGIN
        1 atgaatcctg agcgttctga acgcattgaa atccccgtat tgccgctgcg cgatgtggtg
       61 gtttatccgc acatggtcat ccccttattt gtcgggcggg aaaaatctat ccgttgtctg
      121 gaagcggcga tggaccatga taaaaaaatt atgctggtcg cgcagaaaga agcttcaacg
      181 gatgagccgg gtgtaaacga tcttttcacc gtcgggaccg tggcctctat attgcagatg
      241 ctgaaactgc ctgacggcac cgtcaaagtg ctggtcgagg ggttacagcg cgcgcgtatt
      301 tctgcgctct ctgacaatgg cgaacacttt tctgcgaagg cggagtatct ggagtcgccg
      361 accattgatg agcgggaaca ggaagtgctg gtgcgtactg caatcagcca gttcgaaggc
      421 tacatcaagc tgaacaaaaa aatcccacca gaagtgctga cgtcgctgaa tagcatcgac
      481 gatccggcgc gtctggcgga taccattgct gcacatatgc cgctgaaact ggctgacaaa
      541 cagtctgttc tggagatgtc cgacgttaac gaacgtctgg aatatctgat ggcaatgatg
      601 gaatcggaaa tcgatctgct acaggttgag aaacgcattc gcaaccgcgt taaaaagcag
      661 atggagaaat cccagcgtga gtactatctg aacgagcaaa tgaaagctat tcagaaagaa
      721 ctcggtgaaa tggacgacgc gccggacgaa aacgaagccc tgaagcgcaa aatcgacgcg
      781 gcgaagatgc cgaaagaggc aaaagagaaa gcggaagcag agttgcagaa gctgaaaatg
      841 atgtctccga tgtcggcaga agcgaccgta gtgcgtggtt atatcgactg gatggtacag
      901 gtgccgtgga atgcgcgtag caaggtcaaa aaagacctgc gtcaggcgca ggaaatcctt
      961 gataccgacc attatggtct ggagcgcgtg aaagatcgaa tccttgagta tcttgcggtt
     1021 caaagccgtg tcaacaaaat caagggaccg atcctctgcc tggtagggcc gccgggggta
     1081 ggtaaaacct ctcttggtca gtccattgcc aaagccaccg ggcgtaaata tgtccgtatg
     1141 gcgctgggcg gcgtgcgtga tgaagcggaa atccgtggtc accgccgtac ttacatcggt
     1201 tctatgccgg gtaaactgat ccagaaaatg gcgaaagtgg gcgtgaaaaa cccgctgttc
     1261 ctgctcgatg agatcgacaa aatgtcttct gacatgcgtg gcgatccggc ctctgcactg
     1321 cttgaagtgc tggatccaga gcagaacgta gcgttcagcg accactacct ggaagtggat
     1381 tacgatctca gcgacgtgat gtttgtcgcg acgtcgaact ccatgaacat tccggcaccg
     1441 ctgctggatc gtatggaagt gattcgcctc tccggttata ccgaagatga aaaactgaac
     1501 atcgccaaac gtcacctgct gccgaagcag attgaacgta atgcactgaa aaaaggtgag
     1561 ctgaccgtcg acgatagcgc cattatcggc attattcgtt actacacccg tgaggcgggc
     1621 gtgcgtggtc tggagcgtga aatctccaaa ctgtgtcgca aagcggttaa gcagttactg
     1681 ctcgataagt cattaaaaca tatcgaaatt aacggcgata acctgcatga ctatctcggt
     1741 gttcagcgtt tcgactatgg tcgcgcggat aacgaaaacc gtgtcggtca ggtaaccggt
     1801 ctggcgtgga cggaagtggg cggtgacttg ctgaccattg aaaccgcatg tgttccgggt
     1861 aaaggcaaac tgacctatac cggttcgctc ggcgaagtga tgcaggagtc cattcaggcg
     1921 gcgttaacgg tggttcgtgc gcgtgcggaa aaactgggga tcaaccctga tttttacgaa
     1981 aaacgtgaca tccacgtcca cgtaccggaa ggtgcgacgc cgaaagatgg tccgagtgcc
     2041 ggtattgcta tgtgcaccgc gctggtttct tgcctgaccg gtaacccggt tcgtgccgat
     2101 gtggcaatga ccggtgagat cactctgcgt ggtcaggtac tgccgatcgg tggtttgaaa
     2161 gaaaaactcc tggcagcgca tcgcggcggg attaaaacag tgctaattcc gttcgaaaat
     2221 aaacgcgatc tggaagagat tcctgacaac gtaattgccg atctggacat tcatcctgtg
     2281 aagcgcattg aggaagttct gactctggcg ctgcaaaatg aaccgtctgg tatgcaggtt
     2341 gtgactgcaa aatagtacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     2401 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
     2461 ctcaccttcg ggtgggcctt tctgcgttta ta
//