LOCUS       BBa_K185051             2744 bp    DNA     linear       23-Dec-2024
DEFINITION  promoter(constitutive)+rbs+SmpB+double terminator+pBAD+tmRNA
ACCESSION   BBa_K185051
VERSION     BBa_K185051.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23110
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    62..64
                     /label=start
     misc_feature    542..544
                     /label=stop
     CDS             62..544
                     /label=cds
     CDS             62..544
                     /label=BBa_K185013
     stem_loop       564..607
                     /label=stem_loop
     misc_feature    553..632
                     /label=BBa_B0010
     terminator      553..632
                     /label=BBa_B0010
     stem_loop       648..667
                     /label=T7 TE
     misc_feature    674..674
                     /label=stop
     polyA_site      668..681
                     /label=polya
     misc_feature    641..681
                     /label=BBa_B0012
     terminator      641..681
                     /label=BBa_B0012
     promoter        690..2031
                     /label=BBa_K185052
     CDS             2040..2744
                     /label=BBa_K185054
ORIGIN
        1 tttacggcta gctcagtcct aggtacaatg ctagctacta gagaaagagg agaaatacta
       61 gatgacgaag aaaaaagcac ataaacctgg ttcagcgacc atcgcgctta acaagcgcgc
      121 ccgtcacgaa tactttatcg aagaagagtt cgaagcggga cttgccctgc aaggctggga
      181 agttaaatcc ctgcgcgcag gaaaagccaa tatcagcgac agctacgtcc ttctgcgtga
      241 cggagaggca tttctgtttg gcgctaacat cacgccaatg gccgtggcct ccacgcatgt
      301 ggtgtgcgat cctacccgta cccgcaagtt acttctcaac cagcgcgaac tggactcatt
      361 gtacggtcgc gtcaatcgag aaggctatac cgtagtggcg ctctccctgt actggaaaaa
      421 tgcctggtgc aaagtgaaaa tcggcgtcgc caaaggtaag aaacagcacg ataaacgttc
      481 agatatcaaa gagcgcgaat ggcaggtgga taaagcacgt atcatgaaaa acgcccaccg
      541 ttaatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
      601 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
      661 gtgggccttt ctgcgtttat atactagagc gatgcataat gtgcctgtca aatggacgaa
      721 gcagggattc tgcaaaccct atgctactcc gtcaagccgt caattgtctg attcgttacc
      781 aattatgaca acttgacggc tacatcattc actttttctt cacaaccggc acggaactcg
      841 ctcgggctgg ccccggtgca ttttttaaat acccgcgaga aatagagttg atcgtcaaaa
      901 ccaacattgc gaccgacggt ggcgataggc atccgggtgg tgctcaaaag cagcttcgcc
      961 tggctgatac gttggtcctc gcgccagctt aagacgctaa tccctaactg ctggcggaaa
     1021 agatgtgaca gacgcgacgg cgacaagcaa acatgctgtg cgacgctggc gatatcaaaa
     1081 ttgctgtctg ccaggtgatc gctgatgtac tgacaagcct cgcgtacccg attatccatc
     1141 ggtggatgga gcgactcgtt aatcgcttcc atgcgccgca gtaacaattg ctcaagcaga
     1201 tttatcgcca gcagctccga atagcgccct tccccttgcc cggcgttaat gatttgccca
     1261 aacaggtcgc tgaaatgcgg ctggtgcgct tcatccgggc gaaagaaccc cgtattggca
     1321 aatattgacg gccagttaag ccattcatgc cagtaggcgc gcggacgaaa gtaaacccac
     1381 tggtgatacc attcgcgagc ctccggatga cgaccgtagt gatgaatctc tcctggcggg
     1441 aacagcaaaa tatcacccgg tcggcaaaca aattctcgtc cctgattttt caccaccccc
     1501 tgaccgcgaa tggtgagatt gagaatataa cctttcattc ccagcggtcg gtcgataaaa
     1561 aaatcgagat aaccgttggc ctcaatcggc gttaaacccg ccaccagatg ggcattaaac
     1621 gagtatcccg gcagcagggg atcattttgc gcttcagcca tacttttcat actcccgcca
     1681 ttcagagaag aaaccaattg tccatattgc atcagacatt gccgtcactg cgtcttttac
     1741 tggctcttct cgctaaccaa accggtaacc ccgcttatta aaagcattct gtaacaaagc
     1801 gggaccaaag ccatgacaaa aacgcgtaac aaaagtgtct ataatcacgg cagaaaagtc
     1861 cacattgatt atttgcacgg cgtcacactt tgctatgcca tagcattttt atccataaga
     1921 ttagcggatc ctacctgacg ctttttatcg caactctcta ctgtttctcc atacccgttt
     1981 ttttgggcta gaaataattt tgtttaactt taagaaggag atatacatat gtactagagt
     2041 tgaccagttc ctcaccgcgc ctccctctcc ggcggcgcga atgaacatct tattggctat
     2101 cacatccgac acaaatgttg ccatcccatt gcttaatcga ataaaaatca ggctacatgg
     2161 gtgctaaatc tttaacgata acgccattga ggctggtcat ggcgctcata aatctggtat
     2221 acttaccttt acacattggg gctgattctg gattcgacgg gatttgcgaa acccaaggtg
     2281 catgccgagg ggcggttggc ctcgtaaaaa gccgcaaaaa atagtcgcaa acgacgaaaa
     2341 ctacgcttta gcagcttaat aacctgctta gagccctctc tccctagcct ccgctcttag
     2401 gacggggatc aagagaggtc aaacccaaaa gagatcgcgt ggaagccctg cctggggttg
     2461 aagcgttaaa acttaatcag gctagtttgt tagtggcgtg tccgtccgca gctggcaagc
     2521 gaatgtaaag actgactaag catgtagtac cgaggatgta ggaatttcgg acgcgggttc
     2581 aactcccgcc agctccacca aaattctcca tcggtgatta ccagagtcat ccgatgaagt
     2641 cctaagagcc cgcacggcgc aagccctgcg ggcttttttg tgccctcaat ttgtcccgcg
     2701 aagtccgaag agaactaatt aaatccgaac cttttaggcc catt
//