LOCUS BBa_K185053 4347 bp DNA linear 22-Nov-2024 DEFINITION Promoter116+RBS34+LacI+Double terminator+Plac+RBS31+RelE+RBS30+Lon protease+Double terminator ACCESSION BBa_K185053 VERSION BBa_K185053.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23116 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 CDS 62..1147 /label=BBa_I732100 stem_loop 1167..1210 /label=stem_loop misc_feature 1156..1235 /label=BBa_B0010 terminator 1156..1235 /label=BBa_B0010 stem_loop 1251..1270 /label=T7 TE misc_feature 1277..1277 /label=stop polyA_site 1271..1284 /label=polya misc_feature 1244..1284 /label=BBa_B0012 terminator 1244..1284 /label=BBa_B0012 CDS 1293..1380 /label=end of LacI coding region (inactive) misc_feature 1293..1492 /label=BBa_R0010 misc_feature 1381..1418 /label=CAP binding site promoter 1429..1434 /label=-35 promoter 1453..1458 /label=-10 misc_feature 1458..1492 /label=LacI binding site misc_feature 1465..1465 /label=start promoter 1293..1492 /label=BBa_R0010 misc_feature 1507..1510 /label=conserved RBS 1501..1514 /label=BBa_B0031 misc_feature 1521..1523 /label=start CDS 1524..1805 /label=cds misc_feature 1826..1826 /label=stop misc_feature 1825..1826 /label=mutation misc_feature 1806..1823 /label=His tag CDS 1521..1826 /label=BBa_K185047 RBS 1835..1849 /label=RBS-1\Strong RBS 1842..1846 /label=RBS misc_feature 1835..1849 /label=BBa_B0030 RBS 1835..1849 /label=BBa_B0030 misc_feature 1856..1858 /label=start misc_feature 4208..4210 /label=2353 CDS 1856..4210 /label=cds CDS 1856..4210 /label=BBa_K185049 stem_loop 4230..4273 /label=stem_loop misc_feature 4219..4298 /label=BBa_B0010 terminator 4219..4298 /label=BBa_B0010 stem_loop 4314..4333 /label=T7 TE misc_feature 4340..4340 /label=stop polyA_site 4334..4347 /label=polya misc_feature 4307..4347 /label=BBa_B0012 terminator 4307..4347 /label=BBa_B0012 ORIGIN 1 ttgacagcta gctcagtcct agggactatg ctagctacta gagaaagagg agaaatacta 61 gatgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt 121 ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc 181 ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca 241 gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt 301 cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga 361 acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag 421 tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg 481 cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat 541 tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca 601 gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg 661 ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg 721 gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac 781 tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc 841 cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc 901 atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag 961 cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc 1021 cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg 1081 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca 1141 gtaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct 1201 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt 1261 cgggtgggcc tttctgcgtt tatatactag agcaatacgc aaaccgcctc tccccgcgcg 1321 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga 1381 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat 1441 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca catactagag 1501 tcacacagga aacctactag atggcgtatt ttctggattt tgacgagcgg gcactaaagg 1561 aatggcgaaa gctgggctcg acggtacgtg aacagttgaa aaagaagctg gttgaagtac 1621 ttgagtcacc ccggattgaa gcaaacaagc tccgtggtat gcctgattgt tacaagatta 1681 agctccggtc ttcaggctat cgccttgtat accaggttat agacgagaaa gttgtcgttt 1741 tcgtgatttc tgttgggaaa agagaacgct cggaagtata tagcgaggcg gtcaaacgca 1801 ttctccatca tcatcatcat cactaatact agagattaaa gaggagaaat actagatgaa 1861 tcctgagcgt tctgaacgca ttgaaatccc cgtattgccg ctgcgcgatg tggtggttta 1921 tccgcacatg gtcatcccct tatttgtcgg gcgggaaaaa tctatccgtt gtctggaagc 1981 ggcgatggac catgataaaa aaattatgct ggtcgcgcag aaagaagctt caacggatga 2041 gccgggtgta aacgatcttt tcaccgtcgg gaccgtggcc tctatattgc agatgctgaa 2101 actgcctgac ggcaccgtca aagtgctggt cgaggggtta cagcgcgcgc gtatttctgc 2161 gctctctgac aatggcgaac acttttctgc gaaggcggag tatctggagt cgccgaccat 2221 tgatgagcgg gaacaggaag tgctggtgcg tactgcaatc agccagttcg aaggctacat 2281 caagctgaac aaaaaaatcc caccagaagt gctgacgtcg ctgaatagca tcgacgatcc 2341 ggcgcgtctg gcggatacca ttgctgcaca tatgccgctg aaactggctg acaaacagtc 2401 tgttctggag atgtccgacg ttaacgaacg tctggaatat ctgatggcaa tgatggaatc 2461 ggaaatcgat ctgctgcagg ttgagaaacg cattcgcaac cgcgttaaaa agcagatgga 2521 gaaatcccag cgtgagtact atctgaacga gcaaatgaaa gctattcaga aagaactcgg 2581 tgaaatggac gacgcgccgg acgaaaacga agccctgaag cgcaaaatcg acgcggcgaa 2641 gatgccgaaa gaggcaaaag agaaagcgga agcagagttg cagaagctga aaatgatgtc 2701 tccgatgtcg gcagaagcga ccgtagtgcg tggttatatc gactggatgg tacaggtgcc 2761 gtggaatgcg cgtagcaagg tcaaaaaaga cctgcgtcag gcgcaggaaa tccttgatac 2821 cgaccattat ggtctggagc gcgtgaaaga tcgaatcctt gagtatcttg cggttcaaag 2881 ccgtgtcaac aaaatcaagg gaccgatcct ctgcctggta gggccgccgg gggtaggtaa 2941 aacctctctt ggtcagtcca ttgccaaagc caccgggcgt aaatatgtcc gtatggcgct 3001 gggcggcgtg cgtgatgaag cggaaatccg tggtcaccgc cgtacttaca tcggttctat 3061 gccgggtaaa ctgatccaga aaatggcgaa agtgggcgtg aaaaacccgc tgttcctgct 3121 cgatgagatc gacaaaatgt cttctgacat gcgtggcgat ccggcctctg cactgcttga 3181 agtgctggat ccagagcaga acgtagcgtt cagcgaccac tacctggaag tggattacga 3241 tctcagcgac gtgatgtttg tcgcgacgtc gaactccatg aacattccgg caccgctgct 3301 ggatcgtatg gaagtgattc gcctctccgg ttataccgaa gatgaaaaac tgaacatcgc 3361 caaacgtcac ctgctgccga agcagattga acgtaatgca ctgaaaaaag gtgagctgac 3421 cgtcgacgat agcgccatta tcggcattat tcgttactac acccgtgagg cgggcgtgcg 3481 tggtctggag cgtgaaatct ccaaactgtg tcgcaaagcg gttaagcagt tactgctcga 3541 taagtcatta aaacatatcg aaattaacgg cgataacctg catgactatc tcggtgttca 3601 gcgtttcgac tatggtcgcg cggataacga aaaccgtgtc ggtcaggtaa ccggtctggc 3661 gtggacggaa gtgggcggtg acttgctgac cattgaaacc gcatgtgttc cgggtaaagg 3721 caaactgacc tataccggtt cgctcggcga agtgatgcag gagtccattc aggcggcgtt 3781 aacggtggtt cgtgcgcgtg cggaaaaact ggggatcaac cctgattttt acgaaaaacg 3841 tgacatccac gtccacgtac cggaaggtgc gacgccgaaa gatggtccga gtgccggtat 3901 tgctatgtgc accgcgctgg tttcttgcct gaccggtaac ccggttcgtg ccgatgtggc 3961 aatgaccggt gagatcactc tgcgtggtca ggtactgccg atcggtggtt tgaaagaaaa 4021 actcctggca gcgcatcgcg gcgggattaa aacagtgcta attccgttcg aaaataaacg 4081 cgatctggaa gagattcctg acaacgtaat tgccgatctg gacattcatc ctgtgaagcg 4141 cattgaggaa gttctgactc tggcgctgca aaatgaaccg tctggtatgc aggttgtgac 4201 tgcaaaatag tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg 4261 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac 4321 cttcgggtgg gcctttctgc gtttata //