##gff-version 3
##sequence-region BBa_K185053 1 4347
BBa_K185053	.	terminator	4219	4298	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K185053	.	stem_loop	4230	4273	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K185053	.	engineered_region	4219	4298	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K185053	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K185053	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K185053	.	promoter	1	35	.	+	0	ID=BBa_J23116;Name=BBa_J23116
BBa_K185053	.	terminator	1244	1284	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K185053	.	engineered_region	1244	1284	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K185053	.	stop_codon	1277	1277	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K185053	.	polyA_site	1271	1284	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K185053	.	stem_loop	1251	1270	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K185053	.	promoter	1293	1492	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K185053	.	non_covalent_binding_site	1381	1418	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K185053	.	engineered_region	1293	1492	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K185053	.	non_covalent_binding_site	1458	1492	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K185053	.	start_codon	1465	1465	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K185053	.	promoter	1429	1434	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K185053	.	CDS	1293	1380	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K185053	.	promoter	1453	1458	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K185053	.	terminator	4307	4347	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K185053	.	engineered_region	4307	4347	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K185053	.	stop_codon	4340	4340	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K185053	.	polyA_site	4334	4347	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K185053	.	stem_loop	4314	4333	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K185053	.	CDS	62	1147	.	+	0	ID=BBa_I732100;Name=BBa_I732100
BBa_K185053	.	CDS	1521	1826	.	+	0	ID=BBa_K185047;Name=BBa_K185047
BBa_K185053	.	sequence_alteration	1825	1826	.	+	0	ID=annotation2047034;Name=mutation;Parent=BBa_K185047
BBa_K185053	.	start_codon	1521	1523	.	+	0	ID=annotation2046533;Name=start;Parent=BBa_K185047
BBa_K185053	.	tag	1806	1823	.	+	0	ID=annotation2047133;Name=His tag;Parent=BBa_K185047
BBa_K185053	.	stop_codon	1826	1826	.	+	0	ID=annotation2046574;Name=stop;Parent=BBa_K185047
BBa_K185053	.	CDS	1524	1805	.	+	0	ID=annotation2046560;Name=cds;Parent=BBa_K185047
BBa_K185053	.	ribosome_entry_site	1835	1849	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K185053	.	engineered_region	1835	1849	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K185053	.	ribosome_entry_site	1835	1849	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K185053	.	ribosome_entry_site	1842	1846	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K185053	.	terminator	1156	1235	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K185053	.	stem_loop	1167	1210	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K185053	.	engineered_region	1156	1235	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K185053	.	ribosome_entry_site	1501	1514	.	+	0	ID=BBa_B0031;Name=BBa_B0031
BBa_K185053	.	conserved_region	1507	1510	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_K185053	.	CDS	1856	4210	.	+	0	ID=BBa_K185049;Name=BBa_K185049
BBa_K185053	.	start_codon	1856	1858	.	+	0	ID=annotation2047316;Name=start;Parent=BBa_K185049
BBa_K185053	.	stop_codon	4208	4210	.	+	0	ID=annotation2047317;Name=2353;Parent=BBa_K185049
BBa_K185053	.	CDS	1856	4210	.	+	0	ID=annotation2047318;Name=cds;Parent=BBa_K185049
>BBa_K185053
ttgacagctagctcagtcctagggactatgctagctactagagaaagaggagaaatactagatgaaaccagtaacgtta
tacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcga
aaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaa
acagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatct
cgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgc
acaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagc
tgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaa
gacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagtt
ctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacggga
aggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctg
gttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcgg
tagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgct
ggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctca
ctggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagc
tggcacgacaggtttcccgactggaaagcgggcagtaataatactagagccaggcatcaaataaaacgaaaggctcagt
cgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcggg
tgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagct
ggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcacc
ccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagt
cacacaggaaacctactagatggcgtattttctggattttgacgagcgggcactaaaggaatggcgaaagctgggctcg
acggtacgtgaacagttgaaaaagaagctggttgaagtacttgagtcaccccggattgaagcaaacaagctccgtggta
tgcctgattgttacaagattaagctccggtcttcaggctatcgccttgtataccaggttatagacgagaaagttgtcgt
tttcgtgatttctgttgggaaaagagaacgctcggaagtatatagcgaggcggtcaaacgcattctccatcatcatcat
catcactaatactagagattaaagaggagaaatactagatgaatcctgagcgttctgaacgcattgaaatccccgtatt
gccgctgcgcgatgtggtggtttatccgcacatggtcatccccttatttgtcgggcgggaaaaatctatccgttgtctg
gaagcggcgatggaccatgataaaaaaattatgctggtcgcgcagaaagaagcttcaacggatgagccgggtgtaaacg
atcttttcaccgtcgggaccgtggcctctatattgcagatgctgaaactgcctgacggcaccgtcaaagtgctggtcga
ggggttacagcgcgcgcgtatttctgcgctctctgacaatggcgaacacttttctgcgaaggcggagtatctggagtcg
ccgaccattgatgagcgggaacaggaagtgctggtgcgtactgcaatcagccagttcgaaggctacatcaagctgaaca
aaaaaatcccaccagaagtgctgacgtcgctgaatagcatcgacgatccggcgcgtctggcggataccattgctgcaca
tatgccgctgaaactggctgacaaacagtctgttctggagatgtccgacgttaacgaacgtctggaatatctgatggca
atgatggaatcggaaatcgatctgctgcaggttgagaaacgcattcgcaaccgcgttaaaaagcagatggagaaatccc
agcgtgagtactatctgaacgagcaaatgaaagctattcagaaagaactcggtgaaatggacgacgcgccggacgaaaa
cgaagccctgaagcgcaaaatcgacgcggcgaagatgccgaaagaggcaaaagagaaagcggaagcagagttgcagaag
ctgaaaatgatgtctccgatgtcggcagaagcgaccgtagtgcgtggttatatcgactggatggtacaggtgccgtgga
atgcgcgtagcaaggtcaaaaaagacctgcgtcaggcgcaggaaatccttgataccgaccattatggtctggagcgcgt
gaaagatcgaatccttgagtatcttgcggttcaaagccgtgtcaacaaaatcaagggaccgatcctctgcctggtaggg
ccgccgggggtaggtaaaacctctcttggtcagtccattgccaaagccaccgggcgtaaatatgtccgtatggcgctgg
gcggcgtgcgtgatgaagcggaaatccgtggtcaccgccgtacttacatcggttctatgccgggtaaactgatccagaa
aatggcgaaagtgggcgtgaaaaacccgctgttcctgctcgatgagatcgacaaaatgtcttctgacatgcgtggcgat
ccggcctctgcactgcttgaagtgctggatccagagcagaacgtagcgttcagcgaccactacctggaagtggattacg
atctcagcgacgtgatgtttgtcgcgacgtcgaactccatgaacattccggcaccgctgctggatcgtatggaagtgat
tcgcctctccggttataccgaagatgaaaaactgaacatcgccaaacgtcacctgctgccgaagcagattgaacgtaat
gcactgaaaaaaggtgagctgaccgtcgacgatagcgccattatcggcattattcgttactacacccgtgaggcgggcg
tgcgtggtctggagcgtgaaatctccaaactgtgtcgcaaagcggttaagcagttactgctcgataagtcattaaaaca
tatcgaaattaacggcgataacctgcatgactatctcggtgttcagcgtttcgactatggtcgcgcggataacgaaaac
cgtgtcggtcaggtaaccggtctggcgtggacggaagtgggcggtgacttgctgaccattgaaaccgcatgtgttccgg
gtaaaggcaaactgacctataccggttcgctcggcgaagtgatgcaggagtccattcaggcggcgttaacggtggttcg
tgcgcgtgcggaaaaactggggatcaaccctgatttttacgaaaaacgtgacatccacgtccacgtaccggaaggtgcg
acgccgaaagatggtccgagtgccggtattgctatgtgcaccgcgctggtttcttgcctgaccggtaacccggttcgtg
ccgatgtggcaatgaccggtgagatcactctgcgtggtcaggtactgccgatcggtggtttgaaagaaaaactcctggc
agcgcatcgcggcgggattaaaacagtgctaattccgttcgaaaataaacgcgatctggaagagattcctgacaacgta
attgccgatctggacattcatcctgtgaagcgcattgaggaagttctgactctggcgctgcaaaatgaaccgtctggta
tgcaggttgtgactgcaaaatagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttc
gttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttta
ta