BBa_K185053
type: ComponentDefinition
persistent identity: BBa K185053
display id: BBa_K185053
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter116+RBS34+LacI+Double terminator+Plac+RBS31+RelE+RBS30+Lon protease+Double terminator
created: 2009-10-17T11:00:00Z
modified: 2015-05-08T01:11:07Z
mutable provenance: Promoter, RBS, lacI, plac, double terminator are come from 2009 iGEM biobrick. RelE and Lon protease come from E coli genome DNA
owned by: james
owned by: myers
mutable description: This device composes of promoter, RBS, LacI, Plac, RelE, Lon and so on, which can express relE and Lon protein. The protein expression is controled by lacI.
discontinued: false
dominant: false
experience: experience/None
group u list: _318_
m user id: 0
owner id: 4005
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: We wish the expression of RelE and Lon protease can be controled by LacI.
star: false
creator: Shikun Zhao
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_J23116
component: BBa_I732100
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
component: BBa_B0031
component: BBa_R0010
component: BBa_B0030
component: BBa_K185047
component: BBa_K185049
sequence annotation: BBa_J23116
sequence annotation: BBa_B0034
sequence annotation: BBa_I732100
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0010
sequence annotation: BBa_B0031
sequence annotation: BBa_K185047
sequence annotation: BBa_B0030
sequence annotation: BBa_K185049
sequence: https://synbiohub.org/public/igem/BBa_K185053_sequence/1

BBa_J23116
type: Component
persistent identity: component2051243
display id: component2051243
version: 1
top level: BBa_K185053
definition: BBa_J23116
access: public

BBa_I732100
type: Component
persistent identity: component2051246
display id: component2051246
version: 1
top level: BBa_K185053
definition: LacI
access: public

BBa_B0034
type: Component
persistent identity: component2051245
display id: component2051245
version: 1
top level: BBa_K185053
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component2051249
display id: component2051249
version: 1
top level: BBa_K185053
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2051247
display id: component2051247
version: 1
top level: BBa_K185053
definition: BBa_B0010
access: public

BBa_B0031
type: Component
persistent identity: component2051261
display id: component2051261
version: 1
top level: BBa_K185053
definition: BBa_B0031
access: public

BBa_R0010
type: Component
persistent identity: component2051253
display id: component2051253
version: 1
top level: BBa_K185053
definition: LacI
access: public

BBa_B0030
type: Component
persistent identity: component2051268
display id: component2051268
version: 1
top level: BBa_K185053
definition: BBa_B0030
access: public

BBa_K185047
type: Component
persistent identity: component2051266
display id: component2051266
version: 1
top level: BBa_K185053
definition: BBa_K185047
access: public

BBa_B0010
type: Component
persistent identity: component2051274
display id: component2051274
version: 1
top level: BBa_K185053
definition: BBa_B0010
access: public

BBa_K185049
type: Component
persistent identity: component2051273
display id: component2051273
version: 1
top level: BBa_K185053
definition: BBa_K185049
access: public

BBa_B0012
type: Component
persistent identity: component2051276
display id: component2051276
version: 1
top level: BBa_K185053
definition: BBa_B0012
access: public

BBa_J23116
type: SequenceAnnotation
persistent identity: annotation2051243
display id: annotation2051243
version: 1
top level: BBa_K185053
location: https://synbiohub.org/public/igem/BBa_K185053/annotation2051243/range2051243/1
component: BBa_J23116

https://synbiohub.org/public/igem/BBa_K185053/annotation2051243/range2051243/1
type: Range
persistent identity: range2051243
display id: range2051243
version: 1
top level: BBa_K185053
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2051245
display id: annotation2051245
version: 1
top level: BBa_K185053
location: https://synbiohub.org/public/igem/BBa_K185053/annotation2051245/range2051245/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K185053/annotation2051245/range2051245/1
type: Range
persistent identity: range2051245
display id: range2051245
version: 1
top level: BBa_K185053
direction: direction/reverse
start: 44
end: 55
orientation: inline

BBa_I732100
type: SequenceAnnotation
persistent identity: annotation2051246
display id: annotation2051246
version: 1
top level: BBa_K185053
location: https://synbiohub.org/public/igem/BBa_K185053/annotation2051246/range2051246/1
component: BBa_I732100

https://synbiohub.org/public/igem/BBa_K185053/annotation2051246/range2051246/1
type: Range
persistent identity: range2051246
display id: range2051246
version: 1
top level: BBa_K185053
direction: direction/reverse
start: 62
end: 1147
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2051247
display id: annotation2051247
version: 1
top level: BBa_K185053
location: https://synbiohub.org/public/igem/BBa_K185053/annotation2051247/range2051247/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K185053/annotation2051247/range2051247/1
type: Range
persistent identity: range2051247
display id: range2051247
version: 1
top level: BBa_K185053
direction: direction/forward
start: 1156
end: 1235
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2051249
display id: annotation2051249
version: 1
top level: BBa_K185053
location: https://synbiohub.org/public/igem/BBa_K185053/annotation2051249/range2051249/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K185053/annotation2051249/range2051249/1
type: Range
persistent identity: range2051249
display id: range2051249
version: 1
top level: BBa_K185053
direction: direction/forward
start: 1244
end: 1284
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2051253
display id: annotation2051253
version: 1
top level: BBa_K185053
location: https://synbiohub.org/public/igem/BBa_K185053/annotation2051253/range2051253/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K185053/annotation2051253/range2051253/1
type: Range
persistent identity: range2051253
display id: range2051253
version: 1
top level: BBa_K185053
direction: direction/forward
start: 1293
end: 1492
orientation: inline

BBa_B0031
type: SequenceAnnotation
persistent identity: annotation2051261
display id: annotation2051261
version: 1
top level: BBa_K185053
location: https://synbiohub.org/public/igem/BBa_K185053/annotation2051261/range2051261/1
component: BBa_B0031

https://synbiohub.org/public/igem/BBa_K185053/annotation2051261/range2051261/1
type: Range
persistent identity: range2051261
display id: range2051261
version: 1
top level: BBa_K185053
direction: direction/reverse
start: 1501
end: 1514
orientation: inline

BBa_K185047
type: SequenceAnnotation
persistent identity: annotation2051266
display id: annotation2051266
version: 1
top level: BBa_K185053
location: https://synbiohub.org/public/igem/BBa_K185053/annotation2051266/range2051266/1
component: BBa_K185047

https://synbiohub.org/public/igem/BBa_K185053/annotation2051266/range2051266/1
type: Range
persistent identity: range2051266
display id: range2051266
version: 1
top level: BBa_K185053
direction: direction/reverse
start: 1521
end: 1826
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2051268
display id: annotation2051268
version: 1
top level: BBa_K185053
location: https://synbiohub.org/public/igem/BBa_K185053/annotation2051268/range2051268/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K185053/annotation2051268/range2051268/1
type: Range
persistent identity: range2051268
display id: range2051268
version: 1
top level: BBa_K185053
direction: direction/forward
start: 1835
end: 1849
orientation: inline

BBa_K185049
type: SequenceAnnotation
persistent identity: annotation2051273
display id: annotation2051273
version: 1
top level: BBa_K185053
location: https://synbiohub.org/public/igem/BBa_K185053/annotation2051273/range2051273/1
component: BBa_K185049

https://synbiohub.org/public/igem/BBa_K185053/annotation2051273/range2051273/1
type: Range
persistent identity: range2051273
display id: range2051273
version: 1
top level: BBa_K185053
direction: direction/reverse
start: 1856
end: 4210
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2051274
display id: annotation2051274
version: 1
top level: BBa_K185053
location: https://synbiohub.org/public/igem/BBa_K185053/annotation2051274/range2051274/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K185053/annotation2051274/range2051274/1
type: Range
persistent identity: range2051274
display id: range2051274
version: 1
top level: BBa_K185053
direction: direction/forward
start: 4219
end: 4298
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2051276
display id: annotation2051276
version: 1
top level: BBa_K185053
location: https://synbiohub.org/public/igem/BBa_K185053/annotation2051276/range2051276/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K185053/annotation2051276/range2051276/1
type: Range
persistent identity: range2051276
display id: range2051276
version: 1
top level: BBa_K185053
direction: direction/forward
start: 4307
end: 4347
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_J23116
type: ComponentDefinition
persistent identity: BBa J23116
display id: BBa_J23116
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Later
owned by: james
owned by: myers
mutable description: Later
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J23116_sequence/1

BBa_B0031
type: ComponentDefinition
persistent identity: BBa B0031
display id: BBa_B0031
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.2 (weak) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Medium RBS based on Ron Weiss thesis. Strength considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-1" in figure 4-14 of thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Cho
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0031_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23316
display id: annotation23316
version: 1
top level: BBa_B0031
location: https://synbiohub.org/public/igem/BBa_B0031/annotation23316/range23316/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0031/annotation23316/range23316/1
type: Range
persistent identity: range23316
display id: range23316
version: 1
top level: BBa_B0031
direction: direction/forward
start: 7
end: 10
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa I732100
display id: BBa_I732100
version: 1
was generated by: iGEM to SBOL conversion
description: LacI
created: 2007-10-13T11:00:00Z
modified: 2016-10-18T06:58:21Z
mutable provenance: N/A
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: N/A
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 31248
owner id: 1557
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: true
creator: Zhan Jian
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_I732100_sequence/1

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

BBa_K185047
type: ComponentDefinition
persistent identity: BBa K185047
display id: BBa_K185047
version: 1
was generated by: iGEM to SBOL conversion
description: RelE toxin
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:07Z
mutable provenance: None
owned by: james
owned by: myers
mutable description: None
discontinued: false
dominant: false
experience: experience/Works
group u list: _318_
m user id: 0
owner id: 3967
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Alex Jiang
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: cds
sequence annotation: His tag
sequence annotation: mutation
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_K185047_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2046533
display id: annotation2046533
version: 1
top level: BBa_K185047
location: https://synbiohub.org/public/igem/BBa_K185047/annotation2046533/range2046533/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K185047/annotation2046533/range2046533/1
type: Range
persistent identity: range2046533
display id: range2046533
version: 1
top level: BBa_K185047
direction: direction/forward
start: 1
end: 3
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation2046560
display id: annotation2046560
version: 1
top level: BBa_K185047
location: https://synbiohub.org/public/igem/BBa_K185047/annotation2046560/range2046560/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K185047/annotation2046560/range2046560/1
type: Range
persistent identity: range2046560
display id: range2046560
version: 1
top level: BBa_K185047
direction: direction/forward
start: 4
end: 285
orientation: inline

His tag
type: SequenceAnnotation
persistent identity: annotation2047133
display id: annotation2047133
version: 1
top level: BBa_K185047
location: https://synbiohub.org/public/igem/BBa_K185047/annotation2047133/range2047133/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K185047/annotation2047133/range2047133/1
type: Range
persistent identity: range2047133
display id: range2047133
version: 1
top level: BBa_K185047
direction: direction/forward
start: 286
end: 303
orientation: inline

mutation
type: SequenceAnnotation
persistent identity: annotation2047034
display id: annotation2047034
version: 1
top level: BBa_K185047
location: https://synbiohub.org/public/igem/BBa_K185047/annotation2047034/range2047034/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_K185047/annotation2047034/range2047034/1
type: Range
persistent identity: range2047034
display id: range2047034
version: 1
top level: BBa_K185047
direction: direction/forward
start: 305
end: 306
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2046574
display id: annotation2046574
version: 1
top level: BBa_K185047
location: https://synbiohub.org/public/igem/BBa_K185047/annotation2046574/range2046574/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K185047/annotation2046574/range2046574/1
type: Range
persistent identity: range2046574
display id: range2046574
version: 1
top level: BBa_K185047
direction: direction/forward
start: 306
end: 306
orientation: inline

BBa_K185049
type: ComponentDefinition
persistent identity: BBa K185049
display id: BBa_K185049
version: 1
was generated by: iGEM to SBOL conversion
description: Lon protease
created: 2009-10-15T11:00:00Z
modified: 2015-05-08T01:11:07Z
mutable provenance: None
owned by: james
owned by: myers
mutable description: None
discontinued: false
dominant: false
experience: experience/None
group u list: _318_
m user id: 0
owner id: 3967
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Alex Jiang
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: cds
sequence annotation: 2353
sequence: https://synbiohub.org/public/igem/BBa_K185049_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation2047316
display id: annotation2047316
version: 1
top level: BBa_K185049
location: https://synbiohub.org/public/igem/BBa_K185049/annotation2047316/range2047316/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K185049/annotation2047316/range2047316/1
type: Range
persistent identity: range2047316
display id: range2047316
version: 1
top level: BBa_K185049
direction: direction/forward
start: 1
end: 3
orientation: inline

cds
type: SequenceAnnotation
persistent identity: annotation2047318
display id: annotation2047318
version: 1
top level: BBa_K185049
location: https://synbiohub.org/public/igem/BBa_K185049/annotation2047318/range2047318/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K185049/annotation2047318/range2047318/1
type: Range
persistent identity: range2047318
display id: range2047318
version: 1
top level: BBa_K185049
direction: direction/forward
start: 1
end: 2355
orientation: inline

2353
type: SequenceAnnotation
persistent identity: annotation2047317
display id: annotation2047317
version: 1
top level: BBa_K185049
location: https://synbiohub.org/public/igem/BBa_K185049/annotation2047317/range2047317/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K185049/annotation2047317/range2047317/1
type: Range
persistent identity: range2047317
display id: range2047317
version: 1
top level: BBa_K185049
direction: direction/forward
start: 2353
end: 2355
orientation: inline

https://synbiohub.org/public/igem/BBa_K185053_sequence/1
type: Sequence
persistent identity: BBa K185053 sequence
display id: BBa_K185053_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctagggactatgctagctactagagaaagaggagaaatactagatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagtcacacaggaaacctactagatggcgtattttctggattttgacgagcgggcactaaaggaatggcgaaagctgggctcgacggtacgtgaacagttgaaaaagaagctggttgaagtacttgagtcaccccggattgaagcaaacaagctccgtggtatgcctgattgttacaagattaagctccggtcttcaggctatcgccttgtataccaggttatagacgagaaagttgtcgttttcgtgatttctgttgggaaaagagaacgctcggaagtatatagcgaggcggtcaaacgcattctccatcatcatcatcatcactaatactagagattaaagaggagaaatactagatgaatcctgagcgttctgaacgcattgaaatccccgtattgccgctgcgcgatgtggtggtttatccgcacatggtcatccccttatttgtcgggcgggaaaaatctatccgttgtctggaagcggcgatggaccatgataaaa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23116_sequence/1
type: Sequence
persistent identity: BBa J23116 sequence
display id: BBa_J23116_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctagggactatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0031_sequence/1
type: Sequence
persistent identity: BBa B0031 sequence
display id: BBa_B0031_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaacc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I732100_sequence/1
type: Sequence
persistent identity: BBa I732100 sequence
display id: BBa_I732100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K185047_sequence/1
type: Sequence
persistent identity: BBa K185047 sequence
display id: BBa_K185047_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcgtattttctggattttgacgagcgggcactaaaggaatggcgaaagctgggctcgacggtacgtgaacagttgaaaaagaagctggttgaagtacttgagtcaccccggattgaagcaaacaagctccgtggtatgcctgattgttacaagattaagctccggtcttcaggctatcgccttgtataccaggttatagacgagaaagttgtcgttttcgtgatttctgttgggaaaagagaacgctcggaagtatatagcgaggcggtcaaacgcattctccatcatcatcatcatcactaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K185049_sequence/1
type: Sequence
persistent identity: BBa K185049 sequence
display id: BBa_K185049_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaatcctgagcgttctgaacgcattgaaatccccgtattgccgctgcgcgatgtggtggtttatccgcacatggtcatccccttatttgtcgggcgggaaaaatctatccgttgtctggaagcggcgatggaccatgataaaaaaattatgctggtcgcgcagaaagaagcttcaacggatgagccgggtgtaaacgatcttttcaccgtcgggaccgtggcctctatattgcagatgctgaaactgcctgacggcaccgtcaaagtgctggtcgaggggttacagcgcgcgcgtatttctgcgctctctgacaatggcgaacacttttctgcgaaggcggagtatctggagtcgccgaccattgatgagcgggaacaggaagtgctggtgcgtactgcaatcagccagttcgaaggctacatcaagctgaacaaaaaaatcccaccagaagtgctgacgtcgctgaatagcatcgacgatccggcgcgtctggcggataccattgctgcacatatgccgctgaaactggctgacaaacagtctgttctggagatgtccgacgttaacgaacgtctggaatatctgatggcaatgatggaatcggaaatcgatctgctgcaggttgagaaacgcattcgcaaccgcgttaaaaagcagatggagaaatcccagcgtgagtactatctgaacgagcaaatgaaagctattcagaaagaactcggtgaaatggacgacgcgccggacgaaaacgaagccctgaagcgcaaaatcgacgcggcgaagatgccgaaagaggcaaaagagaaagcggaagcagagttgcagaagctgaaaatgatgtctccgatgtcggcagaagcgaccgtagtgcgtggttatatcgactggatggtacaggtgccgtggaatgcgcgtagcaaggtcaaaaaagacctgcgtcaggcgcaggaaatccttgataccgaccattatggtctggagcgcgtgaaagatcgaatccttgagtatcttgcggttcaaagccgtgtcaacaaaatcaagggaccgatcctctgcctggtagggccgccgggggtaggtaaaacctctcttggtcagtccattgccaaagccaccgggcgtaaatatgtccgtatggcgctgggcggcgtgcgtgatgaagcggaaatccgtggtcaccgccgtacttacatcggttctatgccgggtaaactgatccagaaaatggcgaaagtgggcgtgaaaaacccgctgttcctgctcgatgagatcgacaaaatgtcttctgacatgcgtggcgatccggcctctgcactgcttgaagtgctggatccagagcagaacgtagcgttcagcgaccactacctggaagtggattacgatctcagcgacgtgatgtttgtcgcgacgtcgaactccatgaacattccggcaccgctgctggatcgtatggaagtgattcgcctctccggttataccgaagatgaaaaactgaacatcgccaaacgtcacctgctgccgaagcagattgaacgtaatgcactgaaaaaaggtgagctgaccgtcgacgatagcgccattatcggcattattcgttactacacccgtgaggcgggcgtgcgtggtctggagcgtgaaatctccaaactgtgtcgcaaagcggttaagcagttactgctcgataagtcattaaaacatatcgaaattaacggcgataacctgcatgactatctcggtgttcagcgtttcgactatggtcgcgcggataacgaaaaccgtgtcggtcaggtaaccggtctggcgtggacggaagtgggcggtgacttgctgaccattgaaaccgcatgtgttccgggtaaaggcaaactgacctataccggttcgctcggcgaagtgatgcaggagtccattcaggcggcgttaacggtggttcgtgcgcgtgcggaaaaactggggatcaaccctgatttttacgaaaaacgtgacatccacgtcca…
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z