BBa_K1855000 1 BBa_K1855000 Lpp-OmpA Zif268 2015-09-16T11:00:00Z 2015-09-17T03:10:18Z Lpp-OmpA is originally from E.coli; Zif268 is from mice. A fusion protein of a transmembrane domain (Lpp-OmpA) and a zinc-finger domain (Zif268 1AAY fragment),under pL_lac promotion and containing a C-terminal FLAG tag. It is used to exhibit a zinc-finger dsDNA binding domain complimentary to CCGCCCNCGC on the cell surface of E.coli. false false _2282_ 27125 27125 9 false We had to optimise the codon for E.coli K12, and then alter the codon slightly to remove unwanted cut sites. false Iain Galpin annotation2466654 1 BBa_K864600 T0 Terminator range2466654 1 942 993 annotation2466647 1 B0034 Strong RBS range2466647 1 60 80 annotation2466653 1 3x FLAG range2466653 1 864 935 annotation2466651 1 Zif268 range2466651 1 564 830 annotation2466652 1 Linker range2466652 1 837 860 annotation2466650 1 Linker range2466650 1 522 557 annotation2466649 1 Lpp-OmpA range2466649 1 84 518 annotation2466646 1 pL_lac range2466646 1 1 56 BBa_K1855000_sequence 1 ataaatgtgagcggataacattgacattgtgagcggataacaagatactgagcacattaattaaagaggagaaaggtacccatatgaaagctaccaaactggtgctgggcgcggtgatcctgggttccaccctgctggctggttgttctagcaacgcgaagattgatcaaggtattaacccgtacgttggtttcgaaatgggctacgattggctgggtcgcatgccttacaaaggtagcgtcgagaacggtgcctataaagctcagggcgtgcagctgaccgcgaagctgggttacccgattaccgatgatctggatatctacactcgtctgggcggtatggtttggcgtgccgatactaaatctaacgtctatggtaaaaaccacgacaccggcgtaagcccggtatttgctggtggtgtggagtatgctatcacccctgaaatcgctactcgtctggaataccagtggaccaacaacatcggcgatgcgcacactatcggcacccgcccggataacaccggtggctctggcggtggctccggtggcggctccggcgctagcgaacgtccttatgcttgtccagtggagtcttgcgatcgccgtttctcccgtagcgacgaactgacccgtcacatccgtatccacaccggccagaagccgtttcaatgccgtatctgtatgcgtaacttttcccgcagcgatcacctgactacccacatccgtacccatactggtgaaaaaccgttcgcgtgcgatatctgtggtcgcaaattcgcacgcagcgacgaacgcaaacgtcatactaaaattcacctgcgtcagaaagatatcgatggtggcggttccggcggcggttcccatgattataaagatgacgatgacaaagactacaaggacgatgacgacaaggactataaggacgacgacgataagtgataattgttcagaacgctcggtcttgcacaccgggcgttttttctttgtgagtcca igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z