BBa_K1855001 1 BBa_K1855001 Lpp-OmpA sZF2 2015-09-16T11:00:00Z 2015-09-17T03:11:22Z Lpp-OmpA is originally from E.coli; sZF2 is from http://www.nature.com/nmeth/journal/v10/n5/abs/nmeth.2407.html A fusion protein of a transmembrane domain (Lpp-OmpA) and a zinc-finger domain (sZF2),under pL_lac promotion and containing a C-terminal FLAG tag. It is used to exhibit a zinc-finger dsDNA binding domain complimentary to TGCTGCTTC on the cell surface of E.coli. false false _2282_ 27125 27125 9 false We had to optimise the codon for E.coli K12, and then alter the codon slightly to remove unwanted cut sites. The Lpp-OmpA domain is preceeded by an NdeI cut site, and followed by an AgeI cut site. The sZF2 domain is preceeded by an NheI cut site and followed by a ClaI cut site. false Iain Galpin annotation2466695 1 Lpp-OmpA range2466695 1 84 518 annotation2466713 1 3x FLAG range2466713 1 867 938 annotation2466711 1 sZF2 range2466711 1 564 833 annotation2466693 1 pL_lac range2466693 1 1 56 annotation2466694 1 B0034 Strong RBS range2466694 1 60 80 annotation2466712 1 Linker range2466712 1 840 863 annotation2466714 1 BBa_K864600 T0 Terminator range2466714 1 945 996 annotation2466710 1 Linker range2466710 1 522 557 BBa_K1855001_sequence 1 ataaatgtgagcggataacattgacattgtgagcggataacaagatactgagcacattaattaaagaggagaaaggtacccatatgaaagctaccaaactggtgctgggcgcggtgatcctgggttccaccctgctggctggttgttctagcaacgcgaagattgatcaaggtattaacccgtacgttggtttcgaaatgggctacgattggctgggtcgcatgccttacaaaggtagcgtcgagaacggtgcctataaagctcagggcgtgcagctgaccgcgaagctgggttacccgattaccgatgatctggatatctacactcgtctgggcggtatggtttggcgtgccgatactaaatctaacgtctatggtaaaaaccacgacaccggcgtaagcccggtatttgctggtggtgtggagtatgctatcacccctgaaatcgctactcgtctggaataccagtggaccaacaacatcggcgatgcgcacactatcggcacccgcccggataacaccggtggctctggcggtggctccggtggcggctccggcgctagctcccgtccgggcgaacgtccttttcagtgtcgtatttgcatgcgtaatttctccagccagactcagctggtgcgtcacactcgtacccacaccggcgaaaaaccgtttcaatgtcgtatctgtatgcgcaacttcagccaatccaccactctgaaacgccatctgcgcacccatacgggtgaaaaaccgttccagtgccgcatttgcatgcgcaacttctcccagcgcaacaacctgggtcgccacctgaaaacccatctgcgtggttccatcgatggtggcggttccggcggcggttcccatgattataaagatgacgatgacaaagactacaaggacgatgacgacaaggactataaggacgacgacgataagtgataattgttcagaacgctcggtcttgcacaccgggcgttttttctttgtgagtcca igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z