BBa_K1855002 1 BBa_K1855002 Lpp-OmpA sZF10 2015-09-16T11:00:00Z 2015-09-17T03:11:52Z We had to optimise the codon for E.coli K12, and then alter the codon slightly to remove unwanted cut sites. The Lpp-OmpA domain is preceeded by an NdeI cut site, and followed by an AgeI cut site. The sZF10 domain is preceeded by an NheI cut site and followed by a ClaI cut site. A fusion protein of a transmembrane domain (Lpp-OmpA) and a zinc-finger domain (sZF10),under pL_lac promotion and containing a C-terminal FLAG tag. It is used to exhibit a zinc-finger dsDNA binding domain complimentary to TGCTGCTTC on the cell surface of E.coli. false false _2282_ 27125 27125 9 false Lpp-OmpA is originally from E.coli; sZF10 is from http://www.nature.com/nmeth/journal/v10/n5/abs/nmeth.2407.html false Iain Galpin annotation2466779 1 Lpp-OmpA range2466779 1 84 518 annotation2466781 1 Linker range2466781 1 522 557 annotation2466784 1 Linker range2466784 1 840 863 annotation2466787 1 BBa_K864600 T0 Terminator range2466787 1 945 996 annotation2466777 1 B0034 Strong RBS range2466777 1 60 80 annotation2466785 1 3x FLAG range2466785 1 867 938 annotation2466776 1 pL_lac range2466776 1 1 56 annotation2466782 1 sZF10 range2466782 1 564 833 BBa_K1855002_sequence 1 ataaatgtgagcggataacattgacattgtgagcggataacaagatactgagcacattaattaaagaggagaaaggtacccatatgaaagctaccaaactggtgctgggcgcggtgatcctgggttccaccctgctggctggttgttctagcaacgcgaagattgatcaaggtattaacccgtacgttggtttcgaaatgggctacgattggctgggtcgcatgccttacaaaggtagcgtcgagaacggtgcctataaagctcagggcgtgcagctgaccgcgaagctgggttacccgattaccgatgatctggatatctacactcgtctgggcggtatggtttggcgtgccgatactaaatctaacgtctatggtaaaaaccacgacaccggcgtaagcccggtatttgctggtggtgtggagtatgctatcacccctgaaatcgctactcgtctggaataccagtggaccaacaacatcggcgatgcgcacactatcggcacccgcccggataacaccggtggctctggcggtggctccggtggcggctccggcgctagcagccgtccgggtgagcgtccgttccagtgtcgtctgtgcatgcgcaacttctctcgccagtcgaacctttcgcgccatactcgtacgcacaccggcgaaaaaccatttcagtgtcgcatttgtatgcgtaatttctcgcgcaacgaacatctggtgttgcacctgcgcacgcatactggggaaaaaccatttcaatgccgtatctgcatgcggaactttagccaaaaaacgggattacgtgtacacctgaagactcatctgcgtggctcaatcgatggtggcggttccggcggcggttcccatgattataaagatgacgatgacaaagactacaaggacgatgacgacaaggactataaggacgacgacgataagtgataattgttcagaacgctcggtcttgcacaccgggcgttttttctttgtgagtcca igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z