BBa_K1859003 1 BBa_K1859003 the 3??side of the intron[BBa_K1332005] +HHHHHH linker 2015-09-06T11:00:00Z 2015-09-18T01:55:38Z 4T phage This part consists of mRNA circularization device (5?? side) and linker (amino acid sequence: HHHHHH). It???s the improved mRNA circularization parts (5?? side). The linker may keep a function of coded protein that is translated semi-permanently. The amino acids that consist of the linker are histidine. Because it has some steric hindrance but its hydrophilicity are very strong. The protein coding sequence that is inserted between this device and mRNA circularization device (3??? side) can be circularized. If you circularized the protein coding sequence (Its a stop codon have been removed.) with mRNA circularization device (3?? side) (endless translation)(BBa_K1332009), you can get a circular mRNA that is translated semi-permanently. false false _1707_2287_ 20591 21011 9 false We built a sequence of linker(HHHHHH) into the circuler mRNA. The linker may keep a function of coded protein that is translated semi-permanently. false Wataru Fukuda BBa_K1859003_sequence 1 ggttctacataaatgcctaacgactatccctttggggagtagggtcaagtgactcgaaacgatagacaacttgctttaacaagttggagatatagtctgctctgcatggtgacatgcagctggatataattccggggtaagattaacgaccttatctgaacataatgctaccgtttaatattgcgtcaccaccaccaccacca igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z