LOCUS       BBa_K1859020            1468 bp    DNA     linear       23-Dec-2024
DEFINITION  Histidine tag and RFP linker [GGSGGS*2] semi-permanent generator
ACCESSION   BBa_K1859020
VERSION     BBa_K1859020.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    209..438
                     /label=BBa_K1859007
     misc_feature    451..454
                     /label=conserved
     RBS             447..458
                     /label=BBa_B0034
     CDS             467..1169
                     /label=BBa_K1332002
     misc_feature    1178..1331
                     /label=BBa_K1859011
     stem_loop       1351..1394
                     /label=stem_loop
     misc_feature    1340..1419
                     /label=BBa_B0010
     terminator      1340..1419
                     /label=BBa_B0010
     stem_loop       1435..1454
                     /label=T7 TE
     misc_feature    1461..1461
                     /label=stop
     polyA_site      1455..1468
                     /label=polya
     misc_feature    1428..1468
                     /label=BBa_B0012
     terminator      1428..1468
                     /label=BBa_B0012
     terminator      1340..1468
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagaggg ttctacataa atgcctaacg actatccctt
      241 tggggagtag ggtcaagtga ctcgaaacga tagacaactt gctttaacaa gttggagata
      301 tagtctgctc tgcatggtga catgcagctg gatataattc cggggtaaga ttaacgacct
      361 tatctgaaca taatgctacc gtttaatatt gcgtcatact agaggtggta gtggtggtag
      421 tggtggtagt ggtggtagta ctagagaaag aggagaaata ctagagagat gcatcatcat
      481 catcatcatc atcatatggc ttcctccgaa gacgttatca aagagttcat gcgtttcaaa
      541 gttcgtatgg aaggttccgt taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt
      601 cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg
      661 ttcgcttggg acatcctgtc cccgcagttc cagtacggtt ccaaagctta cgttaaacac
      721 ccggctgaca tcccggacta cctgaaactg tccttcccgg aaggtttcaa atgggaacgt
      781 gttatgaact tcgaagacgg tggtgttgtt accgttaccc aggactcctc cctgcaagac
      841 ggtgagttca tctacaaagt taaactgcgt ggtaccaact tcccgtccga cggtccggtt
      901 atgcagaaaa aaaccatggg ttgggaagct tccaccgaac gtatgtaccc ggaagacggt
      961 gctctgaaag gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca ctacgacgct
     1021 gaagttaaaa ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc
     1081 gacatcaaac tggacatcac ctcccacaac gaagactaca ccatcgttga acagtacgaa
     1141 cgtgctgaag gtcgtcactc caccggtgct actagaggtg gtagtggtgg tagtggtggt
     1201 agtggtggta gtactagagc agagatgttt tcttgggtta attgaggcct gagtataagg
     1261 tgacttatac ttgtaatcta tctaaacggg gaacctctct agtagacaat cccgtgctaa
     1321 attgtaggac ttactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     1381 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
     1441 ccttcgggtg ggcctttctg cgtttata
//