LOCUS       BBa_K1859021            1462 bp    DNA     linear       22-Nov-2024
DEFINITION  Histidine tag and RFP linker [GSGSGS*2] semi-permanent generator
ACCESSION   BBa_K1859021
VERSION     BBa_K1859021.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    209..435
                     /label=BBa_K1859008
     misc_feature    448..451
                     /label=conserved
     RBS             444..455
                     /label=BBa_B0034
     CDS             464..1166
                     /label=BBa_K1332002
     misc_feature    1175..1325
                     /label=BBa_K1859012
     stem_loop       1345..1388
                     /label=stem_loop
     misc_feature    1334..1413
                     /label=BBa_B0010
     terminator      1334..1413
                     /label=BBa_B0010
     stem_loop       1429..1448
                     /label=T7 TE
     misc_feature    1455..1455
                     /label=stop
     polyA_site      1449..1462
                     /label=polya
     misc_feature    1422..1462
                     /label=BBa_B0012
     terminator      1422..1462
                     /label=BBa_B0012
     terminator      1334..1462
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagaggg ttctacataa atgcctaacg actatccctt
      241 tggggagtag ggtcaagtga ctcgaaacga tagacaactt gctttaacaa gttggagata
      301 tagtctgctc tgcatggtga catgcagctg gatataattc cggggtaaga ttaacgacct
      361 tatctgaaca taatgctacc gtttaatatt gcgtcatact agaggtagtg gtagtggtag
      421 tggtagtggt agtggtacta gagaaagagg agaaatacta gagagatgca tcatcatcat
      481 catcatcatc atatggcttc ctccgaagac gttatcaaag agttcatgcg tttcaaagtt
      541 cgtatggaag gttccgttaa cggtcacgag ttcgaaatcg aaggtgaagg tgaaggtcgt
      601 ccgtacgaag gtacccagac cgctaaactg aaagttacca aaggtggtcc gctgccgttc
      661 gcttgggaca tcctgtcccc gcagttccag tacggttcca aagcttacgt taaacacccg
      721 gctgacatcc cggactacct gaaactgtcc ttcccggaag gtttcaaatg ggaacgtgtt
      781 atgaacttcg aagacggtgg tgttgttacc gttacccagg actcctccct gcaagacggt
      841 gagttcatct acaaagttaa actgcgtggt accaacttcc cgtccgacgg tccggttatg
      901 cagaaaaaaa ccatgggttg ggaagcttcc accgaacgta tgtacccgga agacggtgct
      961 ctgaaaggtg aaatcaaaat gcgtctgaaa ctgaaagacg gtggtcacta cgacgctgaa
     1021 gttaaaacca cctacatggc taaaaaaccg gttcagctgc cgggtgctta caaaaccgac
     1081 atcaaactgg acatcacctc ccacaacgaa gactacacca tcgttgaaca gtacgaacgt
     1141 gctgaaggtc gtcactccac cggtgctact agaggtagtg gtagtggtag tggtagtggt
     1201 agtggtacta gagcagagat gttttcttgg gttaattgag gcctgagtat aaggtgactt
     1261 atacttgtaa tctatctaaa cggggaacct ctctagtaga caatcccgtg ctaaattgta
     1321 ggacttacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
     1381 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg
     1441 ggtgggcctt tctgcgttta ta
//