##gff-version 3
##sequence-region BBa_K1859021 1 1462
BBa_K1859021	.	CDS	464	1166	.	+	0	ID=BBa_K1332002;Name=BBa_K1332002
BBa_K1859021	.	ribosome_entry_site	444	455	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1859021	.	conserved_region	448	451	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1859021	.	mature_transcript_region	1175	1325	.	+	0	ID=BBa_K1859012;Name=BBa_K1859012
BBa_K1859021	.	terminator	1334	1462	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1859021	.	terminator	1334	1413	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1859021	.	stem_loop	1345	1388	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1859021	.	engineered_region	1334	1413	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1859021	.	terminator	1422	1462	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1859021	.	stem_loop	1429	1448	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1859021	.	stop_codon	1455	1455	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1859021	.	engineered_region	1422	1462	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1859021	.	polyA_site	1449	1462	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1859021	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K1859021	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1859021	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1859021	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1859021	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1859021	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1859021	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1859021	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1859021	.	mature_transcript_region	209	435	.	+	0	ID=BBa_K1859008;Name=BBa_K1859008
>BBa_K1859021
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagggttctacataaatgcctaacgactatcc
ctttggggagtagggtcaagtgactcgaaacgatagacaacttgctttaacaagttggagatatagtctgctctgcatg
gtgacatgcagctggatataattccggggtaagattaacgaccttatctgaacataatgctaccgtttaatattgcgtc
atactagaggtagtggtagtggtagtggtagtggtagtggtactagagaaagaggagaaatactagagagatgcatcat
catcatcatcatcatcatatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggtt
ccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaa
agttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgtt
aaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcg
aagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtgg
taccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccg
gaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaa
ccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaa
cgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgctactagaggtagtggtagt
ggtagtggtagtggtagtggtactagagcagagatgttttcttgggttaattgaggcctgagtataaggtgacttatac
ttgtaatctatctaaacggggaacctctctagtagacaatcccgtgctaaattgtaggacttactagagccaggcatca
aataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagt
cacactggctcaccttcgggtgggcctttctgcgtttata