LOCUS       BBa_K1859023            1504 bp    DNA     linear       23-Dec-2024
DEFINITION  Histidine tag and RFP linker [GSGSGS*3] semi-permanent generator
ACCESSION   BBa_K1859023
VERSION     BBa_K1859023.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    209..456
                     /label=BBa_K1859010
     misc_feature    469..472
                     /label=conserved
     RBS             465..476
                     /label=BBa_B0034
     CDS             485..1187
                     /label=BBa_K1332002
     misc_feature    1196..1367
                     /label=BBa_K1859014
     stem_loop       1387..1430
                     /label=stem_loop
     misc_feature    1376..1455
                     /label=BBa_B0010
     terminator      1376..1455
                     /label=BBa_B0010
     stem_loop       1471..1490
                     /label=T7 TE
     misc_feature    1497..1497
                     /label=stop
     polyA_site      1491..1504
                     /label=polya
     misc_feature    1464..1504
                     /label=BBa_B0012
     terminator      1464..1504
                     /label=BBa_B0012
     terminator      1376..1504
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagaggg ttctacataa atgcctaacg actatccctt
      241 tggggagtag ggtcaagtga ctcgaaacga tagacaactt gctttaacaa gttggagata
      301 tagtctgctc tgcatggtga catgcagctg gatataattc cggggtaaga ttaacgacct
      361 tatctgaaca taatgctacc gtttaatatt gcgtcatact agaggtagtg gtagtggtag
      421 tggtagtggt agtggtagtg gtagtggtag tggtagtact agagaaagag gagaaatact
      481 agagagatgc atcatcatca tcatcatcat catatggctt cctccgaaga cgttatcaaa
      541 gagttcatgc gtttcaaagt tcgtatggaa ggttccgtta acggtcacga gttcgaaatc
      601 gaaggtgaag gtgaaggtcg tccgtacgaa ggtacccaga ccgctaaact gaaagttacc
      661 aaaggtggtc cgctgccgtt cgcttgggac atcctgtccc cgcagttcca gtacggttcc
      721 aaagcttacg ttaaacaccc ggctgacatc ccggactacc tgaaactgtc cttcccggaa
      781 ggtttcaaat gggaacgtgt tatgaacttc gaagacggtg gtgttgttac cgttacccag
      841 gactcctccc tgcaagacgg tgagttcatc tacaaagtta aactgcgtgg taccaacttc
      901 ccgtccgacg gtccggttat gcagaaaaaa accatgggtt gggaagcttc caccgaacgt
      961 atgtacccgg aagacggtgc tctgaaaggt gaaatcaaaa tgcgtctgaa actgaaagac
     1021 ggtggtcact acgacgctga agttaaaacc acctacatgg ctaaaaaacc ggttcagctg
     1081 ccgggtgctt acaaaaccga catcaaactg gacatcacct cccacaacga agactacacc
     1141 atcgttgaac agtacgaacg tgctgaaggt cgtcactcca ccggtgctac tagaggtagt
     1201 ggtagtggta gtggtagtgg tagtggtagt ggtagtggta gtggtagtac tagagcagag
     1261 atgttttctt gggttaattg aggcctgagt ataaggtgac ttatacttgt aatctatcta
     1321 aacggggaac ctctctagta gacaatcccg tgctaaattg taggacttac tagagccagg
     1381 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     1441 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
     1501 tata
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