LOCUS BBa_K1859029 1420 bp DNA linear 26-Nov-2024 DEFINITION Histidine tag and RFP linker [HHHHHH] semi-permanent generator ACCESSION BBa_K1859029 VERSION BBa_K1859029.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 209..411 /label=BBa_K1859003 misc_feature 424..427 /label=conserved RBS 420..431 /label=BBa_B0034 CDS 440..1142 /label=BBa_K1332002 misc_feature 1151..1283 /label=BBa_K1859006 stem_loop 1303..1346 /label=stem_loop misc_feature 1292..1371 /label=BBa_B0010 terminator 1292..1371 /label=BBa_B0010 stem_loop 1387..1406 /label=T7 TE misc_feature 1413..1413 /label=stop polyA_site 1407..1420 /label=polya misc_feature 1380..1420 /label=BBa_B0012 terminator 1380..1420 /label=BBa_B0012 terminator 1292..1420 /label=BBa_B0015 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagaggg ttctacataa atgcctaacg actatccctt 241 tggggagtag ggtcaagtga ctcgaaacga tagacaactt gctttaacaa gttggagata 301 tagtctgctc tgcatggtga catgcagctg gatataattc cggggtaaga ttaacgacct 361 tatctgaaca taatgctacc gtttaatatt gcgtcaccac caccaccacc atactagaga 421 aagaggagaa atactagaga gatgcatcat catcatcatc atcatcatat ggcttcctcc 481 gaagacgtta tcaaagagtt catgcgtttc aaagttcgta tggaaggttc cgttaacggt 541 cacgagttcg aaatcgaagg tgaaggtgaa ggtcgtccgt acgaaggtac ccagaccgct 601 aaactgaaag ttaccaaagg tggtccgctg ccgttcgctt gggacatcct gtccccgcag 661 ttccagtacg gttccaaagc ttacgttaaa cacccggctg acatcccgga ctacctgaaa 721 ctgtccttcc cggaaggttt caaatgggaa cgtgttatga acttcgaaga cggtggtgtt 781 gttaccgtta cccaggactc ctccctgcaa gacggtgagt tcatctacaa agttaaactg 841 cgtggtacca acttcccgtc cgacggtccg gttatgcaga aaaaaaccat gggttgggaa 901 gcttccaccg aacgtatgta cccggaagac ggtgctctga aaggtgaaat caaaatgcgt 961 ctgaaactga aagacggtgg tcactacgac gctgaagtta aaaccaccta catggctaaa 1021 aaaccggttc agctgccggg tgcttacaaa accgacatca aactggacat cacctcccac 1081 aacgaagact acaccatcgt tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt 1141 gctactagag gccaccacca ccaccaccac acagagatgt tttcttgggt taattgaggc 1201 ctgagtataa ggtgacttat acttgtaatc tatctaaacg gggaacctct ctagtagaca 1261 atcccgtgct aaattgtagg acttactaga gccaggcatc aaataaaacg aaaggctcag 1321 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt 1381 cacactggct caccttcggg tgggcctttc tgcgtttata //