LOCUS       BBa_K1861010             442 bp    DNA     linear       22-Nov-2024
DEFINITION  Expression cassette for S. cerevisiae
ACCESSION   BBa_K1861010
VERSION     BBa_K1861010.1  
FEATURES             Location/Qualifiers
     misc_feature    133..133
                     /label=T->C mutation to avoid SpeI restriction site
     promoter        1..238
                     /label=rpd3 promoter
     promoter        1..238
                     /label=BBa_K1861101
     misc_feature    239..241
                     /label=ATG start codon
     misc_feature    242..259
                     /label=6x His-Tag
     misc_feature    260..265
                     /label=BamHI restriction site
     misc_feature    239..265
                     /label=BBa_K1861102
     misc_feature    311..313
                     /label=TGA stop codon
     misc_feature    305..310
                     /label=SacI restriction site
     CDS             266..313
                     /label=BBa_K1861103
     terminator      314..442
                     /label=BBa_K392003
ORIGIN
        1 gtaatatcaa ctcagagcgt ataggtaaat ttgtaaatat atgtcccata ttttgccttg
       61 aaatttatct ttttatttac ttaccctccc cggctcatag tatctatcga aagggaaaaa
      121 cagaaaagat accagtagtt gattatacat aataaaaatt tgaagtaata accataaagg
      181 ttcataaaac aattgcgcca tacaaaacat tcgtggctac aactcgatat ccgtgcagat
      241 gcatcaccac catcatcacg gatccacgca catttctata agtgcttcac cggcctgcga
      301 acgcgagctc tgagcatgtg ctctgtatgt atataaaact cttgttttct tcttttctct
      361 aaatattctt tccttataca ttaggacctt tgcagcataa attactatac ttctattact
      421 agagcccgcc gccaccatgg ag
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