BBa_K1861103 1 BBa_K1861103 Spacer 2015-09-17T11:00:00Z 2015-09-18T06:17:34Z Coming soon. Coming soon. false false _2291_ 25348 25348 9 false Coming soon. false Vladyslav Vyshnevskyi annotation2476102 1 TGA stop codon range2476102 1 46 48 annotation2476103 1 SacI restriction site range2476103 1 40 45 BBa_K1861101 1 BBa_K1861101 rpd3 promoter 2015-09-17T11:00:00Z 2015-09-19T09:23:03Z Coming soon. Coming soon. false false _2291_ 25348 25348 9 false Coming soon. false Vladyslav Vyshnevskyi annotation2476069 1 rpd3 promoter range2476069 1 1 238 annotation2476062 1 T->C mutation to avoid SpeI restriction site range2476062 1 133 133 BBa_K392003 1 BBa_K392003 yeast ADH1 terminator 2010-10-09T11:00:00Z 2015-05-08T01:12:20Z Registry parts BBa_K105027 (promoter) and BBa_J63003 (Kozak). Engineered 'cyc100 promoter' of <i>Saccharomyces cerevisiae</i> with Kozak sequence attached downstream. false false _528_ 0 6220 9 It's complicated true Assembled by 3A method. false Tadashi Nakamura, Shuhei Yasumoto, Takahiro Saka, Saya Kakuda BBa_K1861010 1 BBa_K1861010 Expression cassette for S. cerevisiae 2015-09-17T11:00:00Z 2015-09-23T05:30:44Z Coming soon. Coming soon. false false _2291_ 25348 25348 9 false Coming soon. false Vladyslav Vyshnevskyi component2471536 1 BBa_K1861103 component2471534 1 BBa_K1861101 component2471537 1 BBa_K392003 component2471535 1 BBa_K1861102 annotation2471537 1 BBa_K392003 range2471537 1 314 442 annotation2471536 1 BBa_K1861103 range2471536 1 266 313 annotation2471534 1 BBa_K1861101 range2471534 1 1 238 annotation2471535 1 BBa_K1861102 range2471535 1 239 265 BBa_K1861102 1 BBa_K1861102 His-Tag 2015-09-17T11:00:00Z 2015-09-19T09:19:53Z Coming soon. Coming soon. false false _2291_ 25348 25348 9 false Coming soon. false Vladyslav Vyshnevskyi annotation2476067 1 6x His-Tag range2476067 1 4 21 annotation2476066 1 ATG start codon range2476066 1 1 3 annotation2476068 1 BamHI restriction site range2476068 1 22 27 BBa_K1861010_sequence 1 gtaatatcaactcagagcgtataggtaaatttgtaaatatatgtcccatattttgccttgaaatttatctttttatttacttaccctccccggctcatagtatctatcgaaagggaaaaacagaaaagataccagtagttgattatacataataaaaatttgaagtaataaccataaaggttcataaaacaattgcgccatacaaaacattcgtggctacaactcgatatccgtgcagatgcatcaccaccatcatcacggatccacgcacatttctataagtgcttcaccggcctgcgaacgcgagctctgagcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctattactagagcccgccgccaccatggag BBa_K1861102_sequence 1 atgcatcaccaccatcatcacggatcc BBa_K1861101_sequence 1 gtaatatcaactcagagcgtataggtaaatttgtaaatatatgtcccatattttgccttgaaatttatctttttatttacttaccctccccggctcatagtatctatcgaaagggaaaaacagaaaagataccagtagttgattatacataataaaaatttgaagtaataaccataaaggttcataaaacaattgcgccatacaaaacattcgtggctacaactcgatatccgtgcag BBa_K392003_sequence 1 gcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctattactagagcccgccgccaccatggag BBa_K1861103_sequence 1 acgcacatttctataagtgcttcaccggcctgcgaacgcgagctctga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z