LOCUS       BBa_K1861020            1468 bp    DNA     linear       22-Dec-2024
DEFINITION  Fusion protein of the TALE constant domain and a SpyTag in an 
            expression cassette
ACCESSION   BBa_K1861020
VERSION     BBa_K1861020.1  
FEATURES             Location/Qualifiers
     misc_feature    133..133
                     /label=T->C mutation to avoid SpeI restriction site
     promoter        1..238
                     /label=rpd3 promoter
     promoter        1..238
                     /label=BBa_K1861101
     misc_feature    239..241
                     /label=ATG start codon
     misc_feature    242..259
                     /label=6x His-Tag
     misc_feature    260..265
                     /label=BamHI restriction site
     misc_feature    239..265
                     /label=BBa_K1861102
     CDS             266..720
                     /label=Front half of the constant domain
     misc_feature    717..727
                     /label=Esp3I restriction site
     misc_feature    767..777
                     /label=Esp3I restriction site
     CDS             774..1291
                     /label=Back half of the constant domain
     CDS             266..1291
                     /label=BBa_K1861201
     misc_feature    1292..1330
                     /label=SpyTag
     misc_feature    1310..1312
                     /label=Reactive Asp for isopeptide forming reaction
     CDS             1292..1330
                     /label=BBa_K1159201
     misc_feature    1331..1336
                     /label=SacI restriction site
     misc_feature    1337..1339
                     /label=TGA stop codon
     misc_feature    1331..1339
                     /label=BBa_K1861104
     terminator      1340..1468
                     /label=BBa_K392003
ORIGIN
        1 gtaatatcaa ctcagagcgt ataggtaaat ttgtaaatat atgtcccata ttttgccttg
       61 aaatttatct ttttatttac ttaccctccc cggctcatag tatctatcga aagggaaaaa
      121 cagaaaagat accagtagtt gattatacat aataaaaatt tgaagtaata accataaagg
      181 ttcataaaac aattgcgcca tacaaaacat tcgtggctac aactcgatat ccgtgcagat
      241 gcatcaccac catcatcacg gatccatggc ccccaagaag aagaggaagg tgggcattca
      301 ccgcggggta cctatggtgg acttgaggac actcggttat tcgcaacagc aacaggagaa
      361 aatcaagcct aaggtcagga gcaccgtcgc gcaacaccac gaggcgcttg tggggcatgg
      421 cttcactcat gcgcatattg tcgcgctttc acagcaccct gcggcgcttg ggacggtggc
      481 tgtcaaatac caagatatga ttgcggccct gcccgaagcc acgcacgagg caattgtagg
      541 ggtcggtaaa cagtggtcgg gagcgcgagc acttgaggcg ctgctgactg tggcgggtga
      601 gcttaggggg cctccgctcc agctcgacac cgggcagctg ctgaagatcg cgaagagagg
      661 gggagtaaca gcggtagagg cagtgcacgc ctggcgcaat gcgctcaccg gtgcccccct
      721 ggagacgcat attatgtata atgctatgga aatcagtttc attcttcgtc tccacgacca
      781 tctggtggcg ttggcatgtc ttggtggacg acccgcgctc gatgcagtca aaaagggtct
      841 gcctcatgct cccgcattga tcaaaagaac caaccggcgg attcccgaga gaacttccca
      901 tcgagtcgcg gatcacgcgc aagtggtccg cgtgctcgga ttcttccagt gtcactccca
      961 ccccgcacaa gcgttcgatg acgccatgac tcaatttggt atgtcgagac acggactgct
     1021 acagctcttt cgtagagtcg gtgtcacaga actcgaggcc cgctcgggca cactgcctcc
     1081 cgcctcccag cggtgggaca ggattctcca agcgagcggt atgaaacgcg cgaagccttc
     1141 acctacgtca actcagacac ctgaccaggc gagccttcat gcgttcgcag actcgctgga
     1201 gagggatttg gacgcgccct cgcccatgca tgaaggggac caaactcgcg cgtcagctag
     1261 ccccaagaag aagagaaagg tgggcagtgg tgctcatatt gtcatggttg atgcttacaa
     1321 gccaactaag gagctctgag catgtgctct gtatgtatat aaaactcttg ttttcttctt
     1381 ttctctaaat attctttcct tatacattag gacctttgca gcataaatta ctatacttct
     1441 attactagag cccgccgcca ccatggag
//