##gff-version 3
##sequence-region BBa_K1861020 1 1468
BBa_K1861020	.	promoter	1	238	.	+	0	ID=BBa_K1861101;Name=BBa_K1861101
BBa_K1861020	.	sequence_alteration	133	133	.	+	0	ID=annotation2476062;Name=T->C mutation to avoid SpeI restriction site;Parent=BBa_K1861101
BBa_K1861020	.	promoter	1	238	.	+	0	ID=annotation2476069;Name=rpd3 promoter;Parent=BBa_K1861101
BBa_K1861020	.	CDS	1292	1330	.	+	0	ID=BBa_K1159201;Name=BBa_K1159201
BBa_K1861020	.	tag	1292	1330	.	+	0	ID=annotation2343481;Name=SpyTag;Parent=BBa_K1159201
BBa_K1861020	.	conserved_region	1310	1312	.	+	0	ID=annotation2343512;Name=Reactive Asp for isopeptide forming reaction;Parent=BBa_K1159201
BBa_K1861020	.	terminator	1340	1468	.	+	0	ID=BBa_K392003;Name=BBa_K392003
BBa_K1861020	.	sequence_feature	1331	1339	.	+	0	ID=BBa_K1861104;Name=BBa_K1861104
BBa_K1861020	.	sequence_feature	1331	1336	.	+	0	ID=annotation2476131;Name=SacI restriction site;Parent=BBa_K1861104
BBa_K1861020	.	stop_codon	1337	1339	.	+	0	ID=annotation2476132;Name=TGA stop codon;Parent=BBa_K1861104
BBa_K1861020	.	tag	239	265	.	+	0	ID=BBa_K1861102;Name=BBa_K1861102
BBa_K1861020	.	tag	242	259	.	+	0	ID=annotation2476067;Name=6x His-Tag;Parent=BBa_K1861102
BBa_K1861020	.	sequence_feature	260	265	.	+	0	ID=annotation2476068;Name=BamHI restriction site;Parent=BBa_K1861102
BBa_K1861020	.	start_codon	239	241	.	+	0	ID=annotation2476066;Name=ATG start codon;Parent=BBa_K1861102
BBa_K1861020	.	CDS	266	1291	.	+	0	ID=BBa_K1861201;Name=BBa_K1861201
BBa_K1861020	.	sequence_feature	717	727	.	+	0	ID=annotation2476196;Name=Esp3I restriction site;Parent=BBa_K1861201
BBa_K1861020	.	sequence_feature	767	777	.	+	0	ID=annotation2476197;Name=Esp3I restriction site;Parent=BBa_K1861201
BBa_K1861020	.	CDS	774	1291	.	+	0	ID=annotation2476198;Name=Back half of the constant domain;Parent=BBa_K1861201
BBa_K1861020	.	CDS	266	720	.	+	0	ID=annotation2476195;Name=Front half of the constant domain;Parent=BBa_K1861201
>BBa_K1861020
gtaatatcaactcagagcgtataggtaaatttgtaaatatatgtcccatattttgccttgaaatttatctttttattta
cttaccctccccggctcatagtatctatcgaaagggaaaaacagaaaagataccagtagttgattatacataataaaaa
tttgaagtaataaccataaaggttcataaaacaattgcgccatacaaaacattcgtggctacaactcgatatccgtgca
gatgcatcaccaccatcatcacggatccatggcccccaagaagaagaggaaggtgggcattcaccgcggggtacctatg
gtggacttgaggacactcggttattcgcaacagcaacaggagaaaatcaagcctaaggtcaggagcaccgtcgcgcaac
accacgaggcgcttgtggggcatggcttcactcatgcgcatattgtcgcgctttcacagcaccctgcggcgcttgggac
ggtggctgtcaaataccaagatatgattgcggccctgcccgaagccacgcacgaggcaattgtaggggtcggtaaacag
tggtcgggagcgcgagcacttgaggcgctgctgactgtggcgggtgagcttagggggcctccgctccagctcgacaccg
ggcagctgctgaagatcgcgaagagagggggagtaacagcggtagaggcagtgcacgcctggcgcaatgcgctcaccgg
tgcccccctggagacgcatattatgtataatgctatggaaatcagtttcattcttcgtctccacgaccatctggtggcg
ttggcatgtcttggtggacgacccgcgctcgatgcagtcaaaaagggtctgcctcatgctcccgcattgatcaaaagaa
ccaaccggcggattcccgagagaacttcccatcgagtcgcggatcacgcgcaagtggtccgcgtgctcggattcttcca
gtgtcactcccaccccgcacaagcgttcgatgacgccatgactcaatttggtatgtcgagacacggactgctacagctc
tttcgtagagtcggtgtcacagaactcgaggcccgctcgggcacactgcctcccgcctcccagcggtgggacaggattc
tccaagcgagcggtatgaaacgcgcgaagccttcacctacgtcaactcagacacctgaccaggcgagccttcatgcgtt
cgcagactcgctggagagggatttggacgcgccctcgcccatgcatgaaggggaccaaactcgcgcgtcagctagcccc
aagaagaagagaaaggtgggcagtggtgctcatattgtcatggttgatgcttacaagccaactaaggagctctgagcat
gtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagc
ataaattactatacttctattactagagcccgccgccaccatggag