LOCUS       BBa_K1861040            2092 bp    DNA     linear       10-May-2024
DEFINITION  Fusion protein of SpyCatcher and RPD3 in an expression cassette
ACCESSION   BBa_K1861040
VERSION     BBa_K1861040.1  
FEATURES             Location/Qualifiers
     misc_feature    133..133
                     /label=T->C mutation to avoid SpeI restriction site
     promoter        1..238
                     /label=rpd3 promoter
     promoter        1..238
                     /label=BBa_K1861101
     misc_feature    239..241
                     /label=ATG start codon
     misc_feature    242..259
                     /label=6x His-Tag
     misc_feature    260..265
                     /label=BamHI restriction site
     misc_feature    239..265
                     /label=BBa_K1861102
     CDS             266..604
                     /label=N-terminal FbaB (modified SpyCatcher)
     misc_feature    365..367
                     /label=Iso --> Glu
     misc_feature    470..472
                     /label=Met --> Tyr
     misc_feature    356..358
                     /label=Reactive Lys for isopeptide forming reaction
     CDS             266..604
                     /label=BBa_K1159200
     CDS             605..628
                     /label=GS-Linker
     CDS             629..1954
                     /label=rpd3
     CDS             605..1954
                     /label=BBa_K1861401
     misc_feature    1955..1960
                     /label=SacI restriction site
     misc_feature    1961..1963
                     /label=TGA stop codon
     misc_feature    1955..1963
                     /label=BBa_K1861104
     terminator      1964..2092
                     /label=BBa_K392003
ORIGIN
        1 gtaatatcaa ctcagagcgt ataggtaaat ttgtaaatat atgtcccata ttttgccttg
       61 aaatttatct ttttatttac ttaccctccc cggctcatag tatctatcga aagggaaaaa
      121 cagaaaagat accagtagtt gattatacat aataaaaatt tgaagtaata accataaagg
      181 ttcataaaac aattgcgcca tacaaaacat tcgtggctac aactcgatat ccgtgcagat
      241 gcatcaccac catcatcacg gatccgttga taccttatca ggtttatcaa gtgagcaagg
      301 tcagtccggt gatatgacaa ttgaagaaga tagtgctacc catattaaat tctcaaaacg
      361 tgatgaggac ggcaaagagt tagctggtgc aactatggag ttgcgtgatt catctggtaa
      421 aactattagt acatggattt cagatggaca agtgaaagat ttctacctgt atccaggaaa
      481 atatacattt gtcgaaaccg cagcaccaga cggttatgag gtagcaactg ctattacctt
      541 tacagttaat gagcaaggtc aggttactgt aaatggcaaa gcaactaaag gtgacgctca
      601 tattggcgga ggaagcggag gtgggagtgt atatgaagca acaccttttg atccgatcac
      661 ggtcaagcca agcgataaaa gacgcgttgc atatttttac gatgcagacg ttgggaacta
      721 tgcttatgga gcaggtcacc cgatgaagcc gcatagaata agaatggcac attcccttat
      781 tatgaattat ggcttgtaca agaagatgga aatttacaga gctaagccgg caacgaaaca
      841 agaaatgtgt cagttccata ctgatgaata cattgatttt ttatcgaggg ttactccaga
      901 taatttagaa atgtttaaaa gagaaagtgt caagtttaat gtcggagatg attgtcctgt
      961 ctttgatgga ttgtatgaat attgcagtat ttctggtggt ggctctatgg aaggagctgc
     1021 tcgtctgaat agaggcaaat gtgatgttgc tgtcaactat gcgggtggtt tgcatcatgc
     1081 aaaaaaatcg gaagcatctg ggttttgtta tttaaatgac atagtactgg gcattattga
     1141 gctactacga taccacccca gagttctgta tattgatatt gatgtgcacc atggtgatgg
     1201 tgtagaggaa gcgttttata caacggatcg tgtcatgaca tgttctttcc acaaatatgg
     1261 tgagtttttc cctggcacag gtgaactgag agatataggg gtgggtgcag gaaaaaacta
     1321 cgcggtcaat gtgccattaa gagacggtat tgacgatgct acgtatagat cagtgtttga
     1381 acctgtgata aaaaaaatta tggaatggta tcaaccttct gctgtcgtgt tacagtgtgg
     1441 tggggactcc ttgtccggcg atcgtcttgg ttgctttaat ctttccatgg aaggccatgc
     1501 taattgtgta aactatgtga aatcctttgg aatcccaatg atggttgttg gtggaggagg
     1561 ctatactatg agaaatgttg caaggacatg gtgctttgaa acaggtctac taaataacgt
     1621 tgtcttggat aaagatttac cgtacaatga atattacgaa tattacggtc cagattataa
     1681 gttaagtgtt agaccttcga atatgttcaa tgtaaatact cccgaatatc ttgacaaggt
     1741 aatgaccaat atatttgcta atttggaaaa cacaaagtat gcccctagtg ttcagttgaa
     1801 tcacacacct agggatgccg aagatttggg tgatgttgaa gaagattctg ccgaggctaa
     1861 agatacgaag ggtggttcgc aatatgcgag ggacctacat gttgagcatg acaatgaatt
     1921 ttatcccaag aagaagagaa aggtgggcag tggagagctc tgagcatgtg ctctgtatgt
     1981 atataaaact cttgttttct tcttttctct aaatattctt tccttataca ttaggacctt
     2041 tgcagcataa attactatac ttctattact agagcccgcc gccaccatgg ag
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