BBa_K1861401 1 BBa_K1861401 GS-Linker and rpd3 2015-09-17T11:00:00Z 2015-09-21T10:31:27Z Coming soon. Coming soon. false false _2291_ 25348 25348 9 false Coming soon. false Vladyslav Vyshnevskyi annotation2476191 1 rpd3 range2476191 1 25 1350 annotation2476190 1 GS-Linker range2476190 1 1 24 BBa_K1159200 1 BBa_K1159200 Splitted and engineered N-terminal FbaB for isopeptide bound formation (SpyCatcher) in RFC[25] 2013-09-13T11:00:00Z 2015-05-08T01:09:31Z ''Streptococcus pyogenes'' This part codes for a protein that recognizes and forms a covalent isopeptide bound to a oligopeptide. That oligopeptide can be found under BBa_K1159201. This part is flanked by RFC[25] pre- and suffix for further protein fusions. false false _1471_ 0 11507 9 In stock false x false Dong-Jiunn Jeffery TRUONG annotation2343349 1 Met --> Tyr range2343349 1 205 207 annotation2343345 1 N-terminal FbaB (modified SpyCatcher) range2343345 1 1 339 annotation2343348 1 Iso --> Glu range2343348 1 100 102 annotation2343366 1 Reactive Lys for isopeptide forming reaction range2343366 1 91 93 BBa_K1861104 1 BBa_K1861104 SacI restriction site and stop codon 2015-09-17T11:00:00Z 2015-09-19T02:45:11Z Coming soon. Coming soon. false false _2291_ 25348 25348 9 false Coming soon. false Vladyslav Vyshnevskyi annotation2476131 1 SacI restriction site range2476131 1 1 6 annotation2476132 1 TGA stop codon range2476132 1 7 9 BBa_K392003 1 BBa_K392003 yeast ADH1 terminator 2010-10-09T11:00:00Z 2015-05-08T01:12:20Z Registry parts BBa_K105027 (promoter) and BBa_J63003 (Kozak). Engineered 'cyc100 promoter' of <i>Saccharomyces cerevisiae</i> with Kozak sequence attached downstream. false false _528_ 0 6220 9 It's complicated true Assembled by 3A method. false Tadashi Nakamura, Shuhei Yasumoto, Takahiro Saka, Saya Kakuda BBa_K1861101 1 BBa_K1861101 rpd3 promoter 2015-09-17T11:00:00Z 2015-09-19T09:23:03Z Coming soon. Coming soon. false false _2291_ 25348 25348 9 false Coming soon. false Vladyslav Vyshnevskyi annotation2476069 1 rpd3 promoter range2476069 1 1 238 annotation2476062 1 T->C mutation to avoid SpeI restriction site range2476062 1 133 133 BBa_K1861102 1 BBa_K1861102 His-Tag 2015-09-17T11:00:00Z 2015-09-19T09:19:53Z Coming soon. Coming soon. false false _2291_ 25348 25348 9 false Coming soon. false Vladyslav Vyshnevskyi annotation2476067 1 6x His-Tag range2476067 1 4 21 annotation2476068 1 BamHI restriction site range2476068 1 22 27 annotation2476066 1 ATG start codon range2476066 1 1 3 BBa_K1861040 1 BBa_K1861040 Fusion protein of SpyCatcher and RPD3 in an expression cassette 2015-09-17T11:00:00Z 2015-09-23T04:21:28Z Coming soon. Coming soon. false false _2291_ 25348 25348 9 false Coming soon. false Vladyslav Vyshnevskyi component2474409 1 BBa_K392003 component2474407 1 BBa_K1861401 component2474401 1 BBa_K1861102 component2474406 1 BBa_K1159200 component2474400 1 BBa_K1861101 component2474408 1 BBa_K1861104 annotation2474401 1 BBa_K1861102 range2474401 1 239 265 annotation2474407 1 BBa_K1861401 range2474407 1 605 1954 annotation2474408 1 BBa_K1861104 range2474408 1 1955 1963 annotation2474400 1 BBa_K1861101 range2474400 1 1 238 annotation2474409 1 BBa_K392003 range2474409 1 1964 2092 annotation2474406 1 BBa_K1159200 range2474406 1 266 604 BBa_K1159200_sequence 1 gttgataccttatcaggtttatcaagtgagcaaggtcagtccggtgatatgacaattgaagaagatagtgctacccatattaaattctcaaaacgtgatgaggacggcaaagagttagctggtgcaactatggagttgcgtgattcatctggtaaaactattagtacatggatttcagatggacaagtgaaagatttctacctgtatccaggaaaatatacatttgtcgaaaccgcagcaccagacggttatgaggtagcaactgctattacctttacagttaatgagcaaggtcaggttactgtaaatggcaaagcaactaaaggtgacgctcatatt BBa_K1861040_sequence 1 gtaatatcaactcagagcgtataggtaaatttgtaaatatatgtcccatattttgccttgaaatttatctttttatttacttaccctccccggctcatagtatctatcgaaagggaaaaacagaaaagataccagtagttgattatacataataaaaatttgaagtaataaccataaaggttcataaaacaattgcgccatacaaaacattcgtggctacaactcgatatccgtgcagatgcatcaccaccatcatcacggatccgttgataccttatcaggtttatcaagtgagcaaggtcagtccggtgatatgacaattgaagaagatagtgctacccatattaaattctcaaaacgtgatgaggacggcaaagagttagctggtgcaactatggagttgcgtgattcatctggtaaaactattagtacatggatttcagatggacaagtgaaagatttctacctgtatccaggaaaatatacatttgtcgaaaccgcagcaccagacggttatgaggtagcaactgctattacctttacagttaatgagcaaggtcaggttactgtaaatggcaaagcaactaaaggtgacgctcatattggcggaggaagcggaggtgggagtgtatatgaagcaacaccttttgatccgatcacggtcaagccaagcgataaaagacgcgttgcatatttttacgatgcagacgttgggaactatgcttatggagcaggtcacccgatgaagccgcatagaataagaatggcacattcccttattatgaattatggcttgtacaagaagatggaaatttacagagctaagccggcaacgaaacaagaaatgtgtcagttccatactgatgaatacattgattttttatcgagggttactccagataatttagaaatgtttaaaagagaaagtgtcaagtttaatgtcggagatgattgtcctgtctttgatggattgtatgaatattgcagtatttctggtggtggctctatggaaggagctgctcgtctgaatagaggcaaatgtgatgttgctgtcaactatgcgggtggtttgcatcatgcaaaaaaatcggaagcatctgggttttgttatttaaatgacatagtactgggcattattgagctactacgataccaccccagagttctgtatattgatattgatgtgcaccatggtgatggtgtagaggaagcgttttatacaacggatcgtgtcatgacatgttctttccacaaatatggtgagtttttccctggcacaggtgaactgagagatataggggtgggtgcaggaaaaaactacgcggtcaatgtgccattaagagacggtattgacgatgctacgtatagatcagtgtttgaacctgtgataaaaaaaattatggaatggtatcaaccttctgctgtcgtgttacagtgtggtggggactccttgtccggcgatcgtcttggttgctttaatctttccatggaaggccatgctaattgtgtaaactatgtgaaatcctttggaatcccaatgatggttgttggtggaggaggctatactatgagaaatgttgcaaggacatggtgctttgaaacaggtctactaaataacgttgtcttggataaagatttaccgtacaatgaatattacgaatattacggtccagattataagttaagtgttagaccttcgaatatgttcaatgtaaatactcccgaatatcttgacaaggtaatgaccaatatatttgctaatttggaaaacacaaagtatgcccctagtgttcagttgaatcacacacctagggatgccgaagatttgggtgatgttgaagaagattctgccgaggctaaagatacgaagggtggttcgcaatatgcgagggacctacatgttgagcatgacaatgaattttatcccaagaagaagagaaaggtgggcagtggagagctctgagcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctattactagagcccgccgccaccatggag BBa_K1861102_sequence 1 atgcatcaccaccatcatcacggatcc BBa_K1861101_sequence 1 gtaatatcaactcagagcgtataggtaaatttgtaaatatatgtcccatattttgccttgaaatttatctttttatttacttaccctccccggctcatagtatctatcgaaagggaaaaacagaaaagataccagtagttgattatacataataaaaatttgaagtaataaccataaaggttcataaaacaattgcgccatacaaaacattcgtggctacaactcgatatccgtgcag BBa_K392003_sequence 1 gcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctattactagagcccgccgccaccatggag BBa_K1861401_sequence 1 ggcggaggaagcggaggtgggagtgtatatgaagcaacaccttttgatccgatcacggtcaagccaagcgataaaagacgcgttgcatatttttacgatgcagacgttgggaactatgcttatggagcaggtcacccgatgaagccgcatagaataagaatggcacattcccttattatgaattatggcttgtacaagaagatggaaatttacagagctaagccggcaacgaaacaagaaatgtgtcagttccatactgatgaatacattgattttttatcgagggttactccagataatttagaaatgtttaaaagagaaagtgtcaagtttaatgtcggagatgattgtcctgtctttgatggattgtatgaatattgcagtatttctggtggtggctctatggaaggagctgctcgtctgaatagaggcaaatgtgatgttgctgtcaactatgcgggtggtttgcatcatgcaaaaaaatcggaagcatctgggttttgttatttaaatgacatagtactgggcattattgagctactacgataccaccccagagttctgtatattgatattgatgtgcaccatggtgatggtgtagaggaagcgttttatacaacggatcgtgtcatgacatgttctttccacaaatatggtgagtttttccctggcacaggtgaactgagagatataggggtgggtgcaggaaaaaactacgcggtcaatgtgccattaagagacggtattgacgatgctacgtatagatcagtgtttgaacctgtgataaaaaaaattatggaatggtatcaaccttctgctgtcgtgttacagtgtggtggggactccttgtccggcgatcgtcttggttgctttaatctttccatggaaggccatgctaattgtgtaaactatgtgaaatcctttggaatcccaatgatggttgttggtggaggaggctatactatgagaaatgttgcaaggacatggtgctttgaaacaggtctactaaataacgttgtcttggataaagatttaccgtacaatgaatattacgaatattacggtccagattataagttaagtgttagaccttcgaatatgttcaatgtaaatactcccgaatatcttgacaaggtaatgaccaatatatttgctaatttggaaaacacaaagtatgcccctagtgttcagttgaatcacacacctagggatgccgaagatttgggtgatgttgaagaagattctgccgaggctaaagatacgaagggtggttcgcaatatgcgagggacctacatgttgagcatgacaatgaattttatcccaagaagaagagaaaggtgggcagtgga BBa_K1861104_sequence 1 gagctctga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z