##gff-version 3
##sequence-region BBa_K1861040 1 2092
BBa_K1861040	.	tag	239	265	.	+	0	ID=BBa_K1861102;Name=BBa_K1861102
BBa_K1861040	.	sequence_feature	260	265	.	+	0	ID=annotation2476068;Name=BamHI restriction site;Parent=BBa_K1861102
BBa_K1861040	.	start_codon	239	241	.	+	0	ID=annotation2476066;Name=ATG start codon;Parent=BBa_K1861102
BBa_K1861040	.	tag	242	259	.	+	0	ID=annotation2476067;Name=6x His-Tag;Parent=BBa_K1861102
BBa_K1861040	.	CDS	605	1954	.	+	0	ID=BBa_K1861401;Name=BBa_K1861401
BBa_K1861040	.	CDS	605	628	.	+	0	ID=annotation2476190;Name=GS-Linker;Parent=BBa_K1861401
BBa_K1861040	.	CDS	629	1954	.	+	0	ID=annotation2476191;Name=rpd3;Parent=BBa_K1861401
BBa_K1861040	.	terminator	1964	2092	.	+	0	ID=BBa_K392003;Name=BBa_K392003
BBa_K1861040	.	sequence_feature	1955	1963	.	+	0	ID=BBa_K1861104;Name=BBa_K1861104
BBa_K1861040	.	sequence_feature	1955	1960	.	+	0	ID=annotation2476131;Name=SacI restriction site;Parent=BBa_K1861104
BBa_K1861040	.	stop_codon	1961	1963	.	+	0	ID=annotation2476132;Name=TGA stop codon;Parent=BBa_K1861104
BBa_K1861040	.	promoter	1	238	.	+	0	ID=BBa_K1861101;Name=BBa_K1861101
BBa_K1861040	.	sequence_alteration	133	133	.	+	0	ID=annotation2476062;Name=T->C mutation to avoid SpeI restriction site;Parent=BBa_K1861101
BBa_K1861040	.	promoter	1	238	.	+	0	ID=annotation2476069;Name=rpd3 promoter;Parent=BBa_K1861101
BBa_K1861040	.	CDS	266	604	.	+	0	ID=BBa_K1159200;Name=BBa_K1159200
BBa_K1861040	.	sequence_alteration	470	472	.	+	0	ID=annotation2343349;Name=Met --> Tyr;Parent=BBa_K1159200
BBa_K1861040	.	conserved_region	356	358	.	+	0	ID=annotation2343366;Name=Reactive Lys for isopeptide forming reaction;Parent=BBa_K1159200
BBa_K1861040	.	CDS	266	604	.	+	0	ID=annotation2343345;Name=N-terminal FbaB (modified SpyCatcher);Parent=BBa_K1159200
BBa_K1861040	.	sequence_alteration	365	367	.	+	0	ID=annotation2343348;Name=Iso --> Glu;Parent=BBa_K1159200
>BBa_K1861040
gtaatatcaactcagagcgtataggtaaatttgtaaatatatgtcccatattttgccttgaaatttatctttttattta
cttaccctccccggctcatagtatctatcgaaagggaaaaacagaaaagataccagtagttgattatacataataaaaa
tttgaagtaataaccataaaggttcataaaacaattgcgccatacaaaacattcgtggctacaactcgatatccgtgca
gatgcatcaccaccatcatcacggatccgttgataccttatcaggtttatcaagtgagcaaggtcagtccggtgatatg
acaattgaagaagatagtgctacccatattaaattctcaaaacgtgatgaggacggcaaagagttagctggtgcaacta
tggagttgcgtgattcatctggtaaaactattagtacatggatttcagatggacaagtgaaagatttctacctgtatcc
aggaaaatatacatttgtcgaaaccgcagcaccagacggttatgaggtagcaactgctattacctttacagttaatgag
caaggtcaggttactgtaaatggcaaagcaactaaaggtgacgctcatattggcggaggaagcggaggtgggagtgtat
atgaagcaacaccttttgatccgatcacggtcaagccaagcgataaaagacgcgttgcatatttttacgatgcagacgt
tgggaactatgcttatggagcaggtcacccgatgaagccgcatagaataagaatggcacattcccttattatgaattat
ggcttgtacaagaagatggaaatttacagagctaagccggcaacgaaacaagaaatgtgtcagttccatactgatgaat
acattgattttttatcgagggttactccagataatttagaaatgtttaaaagagaaagtgtcaagtttaatgtcggaga
tgattgtcctgtctttgatggattgtatgaatattgcagtatttctggtggtggctctatggaaggagctgctcgtctg
aatagaggcaaatgtgatgttgctgtcaactatgcgggtggtttgcatcatgcaaaaaaatcggaagcatctgggtttt
gttatttaaatgacatagtactgggcattattgagctactacgataccaccccagagttctgtatattgatattgatgt
gcaccatggtgatggtgtagaggaagcgttttatacaacggatcgtgtcatgacatgttctttccacaaatatggtgag
tttttccctggcacaggtgaactgagagatataggggtgggtgcaggaaaaaactacgcggtcaatgtgccattaagag
acggtattgacgatgctacgtatagatcagtgtttgaacctgtgataaaaaaaattatggaatggtatcaaccttctgc
tgtcgtgttacagtgtggtggggactccttgtccggcgatcgtcttggttgctttaatctttccatggaaggccatgct
aattgtgtaaactatgtgaaatcctttggaatcccaatgatggttgttggtggaggaggctatactatgagaaatgttg
caaggacatggtgctttgaaacaggtctactaaataacgttgtcttggataaagatttaccgtacaatgaatattacga
atattacggtccagattataagttaagtgttagaccttcgaatatgttcaatgtaaatactcccgaatatcttgacaag
gtaatgaccaatatatttgctaatttggaaaacacaaagtatgcccctagtgttcagttgaatcacacacctagggatg
ccgaagatttgggtgatgttgaagaagattctgccgaggctaaagatacgaagggtggttcgcaatatgcgagggacct
acatgttgagcatgacaatgaattttatcccaagaagaagagaaaggtgggcagtggagagctctgagcatgtgctctg
tatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaatta
ctatacttctattactagagcccgccgccaccatggag