LOCUS       BBa_K1861050            2653 bp    DNA     linear       06-May-2024
DEFINITION  Fusion protein of TALE1 and a SpyTag in an expression cassette
ACCESSION   BBa_K1861050
VERSION     BBa_K1861050.1  
FEATURES             Location/Qualifiers
     misc_feature    133..133
                     /label=T->C mutation to avoid SpeI restriction site
     promoter        1..238
                     /label=rpd3 promoter
     promoter        1..238
                     /label=BBa_K1861101
     misc_feature    239..241
                     /label=ATG start codon
     misc_feature    242..259
                     /label=6x His-Tag
     misc_feature    260..265
                     /label=BamHI restriction site
     misc_feature    239..265
                     /label=BBa_K1861102
     misc_feature    266..268
                     /label=start codon ATG
     CDS             266..724
                     /label=Front constant domain
     CDS             1961..2476
                     /label=Back constant domain
     CDS             725..1960
                     /label=Variable domain
     CDS             266..2476
                     /label=BBa_K1861501
     misc_feature    2477..2515
                     /label=SpyTag
     misc_feature    2495..2497
                     /label=Reactive Asp for isopeptide forming reaction
     CDS             2477..2515
                     /label=BBa_K1159201
     misc_feature    2516..2521
                     /label=SacI restriction site
     misc_feature    2522..2524
                     /label=TGA stop codon
     misc_feature    2516..2524
                     /label=BBa_K1861104
     terminator      2525..2653
                     /label=BBa_K392003
ORIGIN
        1 gtaatatcaa ctcagagcgt ataggtaaat ttgtaaatat atgtcccata ttttgccttg
       61 aaatttatct ttttatttac ttaccctccc cggctcatag tatctatcga aagggaaaaa
      121 cagaaaagat accagtagtt gattatacat aataaaaatt tgaagtaata accataaagg
      181 ttcataaaac aattgcgcca tacaaaacat tcgtggctac aactcgatat ccgtgcagat
      241 gcatcaccac catcatcacg gatccatggc ccccaagaag aagaggaagg tgggcattca
      301 ccgcggggta cctatggtgg acttgaggac actcggttat tcgcaacagc aacaggagaa
      361 aatcaagcct aaggtcagga gcaccgtcgc gcaacaccac gaggcgcttg tggggcatgg
      421 cttcactcat gcgcatattg tcgcgctttc acagcaccct gcggcgcttg ggacggtggc
      481 tgtcaaatac caagatatga ttgcggccct gcccgaagcc acgcacgagg caattgtagg
      541 ggtcggtaaa cagtggtcgg gagcgcgagc acttgaggcg ctgctgactg tggcgggtga
      601 gcttaggggg cctccgctcc agctcgacac cgggcagctg ctgaagatcg cgaagagagg
      661 gggagtaaca gcggtagagg cagtgcacgc ctggcgcaat gcgctcaccg gtgcccccct
      721 gaacctgacc ccggaccaag tggtggctat cgccagccac gatggcggca agcaagcgct
      781 cgaaacggtg cagcggctgt tgccggtgct gtgccaggac catggcctga ccccggacca
      841 agtggtggct atcgccagca acggtggcgg caagcaagcg ctcgaaacgg tgcagcggct
      901 gttgccggtg ctgtgccagg accatggcct gactccggac caagtggtgg ctatcgccag
      961 ccacgatggc ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca
     1021 ggaccatggc ctgactccgg accaagtggt ggctatcgcc agccacgatg gcggcaagca
     1081 agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg gcctgacccc
     1141 ggaccaagtg gtggctatcg ccagcaacat tggcggcaag caagcgctcg aaacggtgca
     1201 gcggctgttg ccggtgctgt gccaggacca tggcctgact ccggaccaag tggtggctat
     1261 cgccagccac gatggcggca agcaagcgct cgaaacggtg cagcggctgt tgccggtgct
     1321 gtgccaggac catggcctga ctccggacca agtggtggct atcgccagcc acgatggcgg
     1381 caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg accatggcct
     1441 gactccggac caagtggtgg ctatcgccag ccacgatggc ggcaagcaag cgctcgaaac
     1501 ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc ctgactccgg accaagtggt
     1561 ggctatcgcc agccacgatg gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc
     1621 ggtgctgtgc caggaccatg gcctgacccc ggaccaagtg gtggctatcg ccagcaacat
     1681 tggcggcaag caagcgctcg aaacggtgca gcggctgttg ccggtgctgt gccaggacca
     1741 tggcctgacc ccggaccaag tggtggctat cgccagcaac ggtggcggca agcaagcgct
     1801 cgaaacggtg cagcggctgt tgccggtgct gtgccaggac catggcctga ccccggacca
     1861 agtggtggct atcgccagca acggtggcgg caagcaagcg ctcgaaagca ttgtggccca
     1921 gctgagccgg cctgatccgg cgttggccgc gttgaccaac gaccatctgg tggcgttggc
     1981 atgtcttggt ggacgacccg cgctcgatgc agtcaaaaag ggtctgcctc atgctcccgc
     2041 attgatcaaa agaaccaacc ggcggattcc cgagagaact tcccatcgag tcgcggatca
     2101 cgcgcaagtg gtccgcgtgc tcggattctt ccagtgtcac tcccaccccg cacaagcgtt
     2161 cgatgacgcc atgactcaat ttggtatgtc gagacacgga ctgctacagc tctttcgtag
     2221 agtcggtgtc acagaactcg aggcccgctc gggcacactg cctcccgcct cccagcggtg
     2281 ggacaggatt ctccaagcga gcggtatgaa acgcgcgaag ccttcaccta cgtcaactca
     2341 gacacctgac caggcgagcc ttcatgcgtt cgcagactcg ctggagaggg atttggacgc
     2401 gccctcgccc atgcatgaag gggaccaaac tcgcgcgtca gctagcccca agaagaagag
     2461 aaaggtgggc agtggtgctc atattgtcat ggttgatgct tacaagccaa ctaaggagct
     2521 ctgagcatgt gctctgtatg tatataaaac tcttgttttc ttcttttctc taaatattct
     2581 ttccttatac attaggacct ttgcagcata aattactata cttctattac tagagcccgc
     2641 cgccaccatg gag
//