BBa_K1861104 1 BBa_K1861104 SacI restriction site and stop codon 2015-09-17T11:00:00Z 2015-09-19T02:45:11Z Coming soon. Coming soon. false false _2291_ 25348 25348 9 false Coming soon. false Vladyslav Vyshnevskyi annotation2476131 1 SacI restriction site range2476131 1 1 6 annotation2476132 1 TGA stop codon range2476132 1 7 9 BBa_K1861601 1 TALE2 TALE that binds the sequence T GTCGTAACAACT 2015-09-17T11:00:00Z 2015-09-23T03:13:47Z Coming soon. Is it just me or why can't I edit the long description? false false _2291_ 25348 25348 9 false Coming soon. false Vladyslav Vyshnevskyi annotation2476632 1 Variable domain range2476632 1 460 1695 annotation2476630 1 start codon ATG range2476630 1 1 3 annotation2476633 1 Back constant domain range2476633 1 1696 2211 annotation2476631 1 Front constant domain range2476631 1 1 459 BBa_K1861101 1 BBa_K1861101 rpd3 promoter 2015-09-17T11:00:00Z 2015-09-19T09:23:03Z Coming soon. Coming soon. false false _2291_ 25348 25348 9 false Coming soon. false Vladyslav Vyshnevskyi annotation2476069 1 rpd3 promoter range2476069 1 1 238 annotation2476062 1 T->C mutation to avoid SpeI restriction site range2476062 1 133 133 BBa_K392003 1 BBa_K392003 yeast ADH1 terminator 2010-10-09T11:00:00Z 2015-05-08T01:12:20Z Registry parts BBa_K105027 (promoter) and BBa_J63003 (Kozak). Engineered 'cyc100 promoter' of <i>Saccharomyces cerevisiae</i> with Kozak sequence attached downstream. false false _528_ 0 6220 9 It's complicated true Assembled by 3A method. false Tadashi Nakamura, Shuhei Yasumoto, Takahiro Saka, Saya Kakuda BBa_K1159201 1 BBa_K1159201 Splitted and engineered C-terminal FbaB for isopeptide bound formation (SpyTag) in RFC[25] 2013-09-13T11:00:00Z 2015-05-08T01:09:31Z ''Streptococcus pyogenes'' This part codes for a oligopeptide that is recognized and bound by a covalent isopeptide bound to a protein, called SpyCatcher. That catcher protein for this oligopeptdie can be found under BBa_K1159200. This part is flanked by RFC[25] pre- and suffix for further protein fusions. false false _1471_ 0 11507 9 In stock false x false Dong-Jiunn Jeffery TRUONG annotation2343512 1 Reactive Asp for isopeptide forming reaction range2343512 1 19 21 annotation2343481 1 SpyTag range2343481 1 1 39 BBa_K1861102 1 BBa_K1861102 His-Tag 2015-09-17T11:00:00Z 2015-09-19T09:19:53Z Coming soon. Coming soon. false false _2291_ 25348 25348 9 false Coming soon. false Vladyslav Vyshnevskyi annotation2476068 1 BamHI restriction site range2476068 1 22 27 annotation2476067 1 6x His-Tag range2476067 1 4 21 annotation2476066 1 ATG start codon range2476066 1 1 3 BBa_K1861060 1 BBa_K1861060 Fusion protein of TALE2 and a SpyTag in an expression cassette 2015-09-17T11:00:00Z 2015-09-23T04:10:41Z It keeps saying I have no permission to edit this. Why can't I edit these descriptions? false false _2291_ 25348 25348 9 false Coming soon, if I am allowed to edit this. false Vladyslav Vyshnevskyi component2475046 1 BBa_K1861104 component2475041 1 BBa_K1861102 component2475045 1 BBa_K1159201 component2475042 1 BBa_K1861601 component2475040 1 BBa_K1861101 component2475047 1 BBa_K392003 annotation2475046 1 BBa_K1861104 range2475046 1 2515 2523 annotation2475040 1 BBa_K1861101 range2475040 1 1 238 annotation2475042 1 BBa_K1861601 range2475042 1 266 2475 annotation2475045 1 BBa_K1159201 range2475045 1 2476 2514 annotation2475047 1 BBa_K392003 range2475047 1 2524 2652 annotation2475041 1 BBa_K1861102 range2475041 1 239 265 BBa_K1861060_sequence 1 gtaatatcaactcagagcgtataggtaaatttgtaaatatatgtcccatattttgccttgaaatttatctttttatttacttaccctccccggctcatagtatctatcgaaagggaaaaacagaaaagataccagtagttgattatacataataaaaatttgaagtaataaccataaaggttcataaaacaattgcgccatacaaaacattcgtggctacaactcgatatccgtgcagatgcatcaccaccatcatcacggatccatggcccccaagaagaagaggaaggtgggcattcaccgcggggtacctatggtggacttgaggacactcggttattcgcaacagcaacaggagaaaatcaagcctaaggtcaggagcaccgtcgcgcaacaccacgaggcgcttgtggggcatggcttcactcatgcgcatattgtcgcgctttcacagcaccctgcggcgcttgggacggtggctgtcaaataccaagatatgattgcggccctgcccgaagccacgcacgaggcaattgtaggggtcggtaaacagtggtcgggagcgcgagcacttgaggcgctgctgactgtggcgggtgagcttagggggcctccgctccagctcgacaccgggcagctgctgaagatcgcgaagagagggggagtaacagcggtagaggcagtgcacgcctggcgcaatgcgctcaccggtgcccccctgaatctaacccctgaccaggtagttgccatcgcttccaaccacggcggaaaacaggccctagaaaccgttcagcgtttgttgccagtcttatgccaagaccatggattgactccagaccaagtcgtcgctatcgcctcaaacggtggagggaagcaagcattggaaacagtccaaagactgctacctgtcctgtgtcaggaccacgggttaactccggaccaggttgtggccatagcctctcatgatggtggtaagcaggcacttgaaacggtacagagactactacccgtgctttgtcaggaccacggacttacccccgatcaagtagtagcgattgcgagtaaccatggcggtaaacaagcccttgaaactgtgcaaagattgctgcctgtattgtgccaggatcacgggcttacaccggatcaggtcgtagcaatagcatctaatggaggtggaaagcaagctttggagactgttcaacgtttattgcctgtcttgtgtcaagatcatgggctgacacccgaccaagtggtagccattgcctctaacattgggggcaaacaggctttagagacagtgcagcgtcttttgcccgttttgtgcaggatcacggcttgactccggatcaggtcgtggcgatagcgagcaatataggcgggaaacaggcgctagaaactgtccaaagattattaccggttttatgtcaggaccatgggctaacccctgatcaagtcgtcgccatagcgtcccacgacggaggtaaacaagcgttggagactgtgcagcgtctattgcccgtgctttgtcaagaccacggacttacgccagaccaggttgtggctattgcaagtaacataggtggcaaacaggctttggaaacggtacaaagactactgccagtcttatgccaagatcatggattgacacctgaccaagtggtggccattgcatctaatatcggggggaagcaagctcttgagacagttcaacgtttgttaccagttctatgtcaagaccacggtcttacacccgaccaggtagttgccatcgcatctcatgatggcggtaagcaagccctagaaacggttcagagattgctgccggtactttgccaggatcacgggttaacaccggaccaagttgtcgcgattgcctctaatggtggaggaaagcaggccctggaatctattgttgcacagctttcaagaccagatccggctctggctgcattgactaacgaccatctggtggcgttggcatgtcttggtggacgacccgcgctcgatgcagtcaaaaagggtctgcctcatgctcccgcattgatcaaaagaaccaaccggcggattcccgagagaacttcccatcgagtcgcggatcacgcgcaagtggtccgcgtgctcggattcttccagtgtcactcccaccccgcacaagcgttcgatgacgccatgactcaatttggtatgtcgagacacggactgctacagctctttcgtagagtcggtgtcacagaactcgaggcccgctcgggcacactgcctcccgcctcccagcggtgggacaggattctccaagcgagcggtatgaaacgcgcgaagccttcacctacgtcaactcagacacctgaccaggcgagccttcatgcgttcgcagactcgctggagagggatttggacgcgccctcgcccatgcatgaaggggaccaaactcgcgcgtcagctagccccaagaagaagagaaaggtgggcagtggtgctcatattgtcatggttgatgcttacaagccaactaaggagctctgagcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctattactagagcccgccgccaccatggag BBa_K1861102_sequence 1 atgcatcaccaccatcatcacggatcc BBa_K1861101_sequence 1 gtaatatcaactcagagcgtataggtaaatttgtaaatatatgtcccatattttgccttgaaatttatctttttatttacttaccctccccggctcatagtatctatcgaaagggaaaaacagaaaagataccagtagttgattatacataataaaaatttgaagtaataaccataaaggttcataaaacaattgcgccatacaaaacattcgtggctacaactcgatatccgtgcag BBa_K392003_sequence 1 gcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctattactagagcccgccgccaccatggag BBa_K1861104_sequence 1 gagctctga BBa_K1159201_sequence 1 gctcatattgtcatggttgatgcttacaagccaactaag BBa_K1861601_sequence 1 atggcccccaagaagaagaggaaggtgggcattcaccgcggggtacctatggtggacttgaggacactcggttattcgcaacagcaacaggagaaaatcaagcctaaggtcaggagcaccgtcgcgcaacaccacgaggcgcttgtggggcatggcttcactcatgcgcatattgtcgcgctttcacagcaccctgcggcgcttgggacggtggctgtcaaataccaagatatgattgcggccctgcccgaagccacgcacgaggcaattgtaggggtcggtaaacagtggtcgggagcgcgagcacttgaggcgctgctgactgtggcgggtgagcttagggggcctccgctccagctcgacaccgggcagctgctgaagatcgcgaagagagggggagtaacagcggtagaggcagtgcacgcctggcgcaatgcgctcaccggtgcccccctgaatctaacccctgaccaggtagttgccatcgcttccaaccacggcggaaaacaggccctagaaaccgttcagcgtttgttgccagtcttatgccaagaccatggattgactccagaccaagtcgtcgctatcgcctcaaacggtggagggaagcaagcattggaaacagtccaaagactgctacctgtcctgtgtcaggaccacgggttaactccggaccaggttgtggccatagcctctcatgatggtggtaagcaggcacttgaaacggtacagagactactacccgtgctttgtcaggaccacggacttacccccgatcaagtagtagcgattgcgagtaaccatggcggtaaacaagcccttgaaactgtgcaaagattgctgcctgtattgtgccaggatcacgggcttacaccggatcaggtcgtagcaatagcatctaatggaggtggaaagcaagctttggagactgttcaacgtttattgcctgtcttgtgtcaagatcatgggctgacacccgaccaagtggtagccattgcctctaacattgggggcaaacaggctttagagacagtgcagcgtcttttgcccgttttgtgccaggatcacggcttgactccggatcaggtcgtggcgatagcgagcaatataggcgggaaacaggcgctagaaactgtccaaagattattaccggttttatgtcaggaccatgggctaacccctgatcaagtcgtcgccatagcgtcccacgacggaggtaaacaagcgttggagactgtgcagcgtctattgcccgtgctttgtcaagaccacggacttacgccagaccaggttgtggctattgcaagtaacataggtggcaaacaggctttggaaacggtacaaagactactgccagtcttatgccaagatcatggattgacacctgaccaagtggtggccattgcatctaatatcggggggaagcaagctcttgagacagttcaacgtttgttaccagttctatgtcaagaccacggtcttacacccgaccaggtagttgccatcgcatctcatgatggcggtaagcaagccctagaaacggttcagagattgctgccggtactttgccaggatcacgggttaacaccggaccaagttgtcgcgattgcctctaatggtggaggaaagcaggccctggaatctattgttgcacagctttcaagaccagatccggctctggctgcattgactaacgaccatctggtggcgttggcatgtcttggtggacgacccgcgctcgatgcagtcaaaaagggtctgcctcatgctcccgcattgatcaaaagaaccaaccggcggattcccgagagaacttcccatcgagtcgcggatcacgcgcaagtggtccgcgtgctcggattcttccagtgtcactcccaccccgcacaagcgttcgatgacgccatgactcaatttggtatgtcgagacacggactgctacagctctttcgtagagtcggtgtcacagaactcgaggcccgctcgggcacactgcctcccgcctcccagcggtgggacaggattctccaagcgagcggtatgaaacgcgcgaagccttcacctacgtcaactcagacacctgaccaggcgagccttcatgcgttcgcagactcgctggagagggatttggacgcgccctcgcccatgcatgaaggggaccaaactcgcgcgtcagctagccccaagaagaagagaaaggtgggcagtggt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z