##gff-version 3
##sequence-region BBa_K1861060 1 2652
BBa_K1861060	.	sequence_feature	2515	2523	.	+	0	ID=BBa_K1861104;Name=BBa_K1861104
BBa_K1861060	.	stop_codon	2521	2523	.	+	0	ID=annotation2476132;Name=TGA stop codon;Parent=BBa_K1861104
BBa_K1861060	.	sequence_feature	2515	2520	.	+	0	ID=annotation2476131;Name=SacI restriction site;Parent=BBa_K1861104
BBa_K1861060	.	terminator	2524	2652	.	+	0	ID=BBa_K392003;Name=BBa_K392003
BBa_K1861060	.	CDS	2476	2514	.	+	0	ID=BBa_K1159201;Name=BBa_K1159201
BBa_K1861060	.	tag	2476	2514	.	+	0	ID=annotation2343481;Name=SpyTag;Parent=BBa_K1159201
BBa_K1861060	.	conserved_region	2494	2496	.	+	0	ID=annotation2343512;Name=Reactive Asp for isopeptide forming reaction;Parent=BBa_K1159201
BBa_K1861060	.	CDS	266	2475	.	+	0	ID=BBa_K1861601;Name=BBa_K1861601
BBa_K1861060	.	CDS	266	724	.	+	0	ID=annotation2476631;Name=Front constant domain;Parent=BBa_K1861601
BBa_K1861060	.	start_codon	266	268	.	+	0	ID=annotation2476630;Name=start codon ATG;Parent=BBa_K1861601
BBa_K1861060	.	CDS	1961	2476	.	+	0	ID=annotation2476633;Name=Back constant domain;Parent=BBa_K1861601
BBa_K1861060	.	CDS	725	1960	.	+	0	ID=annotation2476632;Name=Variable domain;Parent=BBa_K1861601
BBa_K1861060	.	promoter	1	238	.	+	0	ID=BBa_K1861101;Name=BBa_K1861101
BBa_K1861060	.	sequence_alteration	133	133	.	+	0	ID=annotation2476062;Name=T->C mutation to avoid SpeI restriction site;Parent=BBa_K1861101
BBa_K1861060	.	promoter	1	238	.	+	0	ID=annotation2476069;Name=rpd3 promoter;Parent=BBa_K1861101
BBa_K1861060	.	tag	239	265	.	+	0	ID=BBa_K1861102;Name=BBa_K1861102
BBa_K1861060	.	sequence_feature	260	265	.	+	0	ID=annotation2476068;Name=BamHI restriction site;Parent=BBa_K1861102
BBa_K1861060	.	start_codon	239	241	.	+	0	ID=annotation2476066;Name=ATG start codon;Parent=BBa_K1861102
BBa_K1861060	.	tag	242	259	.	+	0	ID=annotation2476067;Name=6x His-Tag;Parent=BBa_K1861102
>BBa_K1861060
gtaatatcaactcagagcgtataggtaaatttgtaaatatatgtcccatattttgccttgaaatttatctttttattta
cttaccctccccggctcatagtatctatcgaaagggaaaaacagaaaagataccagtagttgattatacataataaaaa
tttgaagtaataaccataaaggttcataaaacaattgcgccatacaaaacattcgtggctacaactcgatatccgtgca
gatgcatcaccaccatcatcacggatccatggcccccaagaagaagaggaaggtgggcattcaccgcggggtacctatg
gtggacttgaggacactcggttattcgcaacagcaacaggagaaaatcaagcctaaggtcaggagcaccgtcgcgcaac
accacgaggcgcttgtggggcatggcttcactcatgcgcatattgtcgcgctttcacagcaccctgcggcgcttgggac
ggtggctgtcaaataccaagatatgattgcggccctgcccgaagccacgcacgaggcaattgtaggggtcggtaaacag
tggtcgggagcgcgagcacttgaggcgctgctgactgtggcgggtgagcttagggggcctccgctccagctcgacaccg
ggcagctgctgaagatcgcgaagagagggggagtaacagcggtagaggcagtgcacgcctggcgcaatgcgctcaccgg
tgcccccctgaatctaacccctgaccaggtagttgccatcgcttccaaccacggcggaaaacaggccctagaaaccgtt
cagcgtttgttgccagtcttatgccaagaccatggattgactccagaccaagtcgtcgctatcgcctcaaacggtggag
ggaagcaagcattggaaacagtccaaagactgctacctgtcctgtgtcaggaccacgggttaactccggaccaggttgt
ggccatagcctctcatgatggtggtaagcaggcacttgaaacggtacagagactactacccgtgctttgtcaggaccac
ggacttacccccgatcaagtagtagcgattgcgagtaaccatggcggtaaacaagcccttgaaactgtgcaaagattgc
tgcctgtattgtgccaggatcacgggcttacaccggatcaggtcgtagcaatagcatctaatggaggtggaaagcaagc
tttggagactgttcaacgtttattgcctgtcttgtgtcaagatcatgggctgacacccgaccaagtggtagccattgcc
tctaacattgggggcaaacaggctttagagacagtgcagcgtcttttgcccgttttgtgcaggatcacggcttgactcc
ggatcaggtcgtggcgatagcgagcaatataggcgggaaacaggcgctagaaactgtccaaagattattaccggtttta
tgtcaggaccatgggctaacccctgatcaagtcgtcgccatagcgtcccacgacggaggtaaacaagcgttggagactg
tgcagcgtctattgcccgtgctttgtcaagaccacggacttacgccagaccaggttgtggctattgcaagtaacatagg
tggcaaacaggctttggaaacggtacaaagactactgccagtcttatgccaagatcatggattgacacctgaccaagtg
gtggccattgcatctaatatcggggggaagcaagctcttgagacagttcaacgtttgttaccagttctatgtcaagacc
acggtcttacacccgaccaggtagttgccatcgcatctcatgatggcggtaagcaagccctagaaacggttcagagatt
gctgccggtactttgccaggatcacgggttaacaccggaccaagttgtcgcgattgcctctaatggtggaggaaagcag
gccctggaatctattgttgcacagctttcaagaccagatccggctctggctgcattgactaacgaccatctggtggcgt
tggcatgtcttggtggacgacccgcgctcgatgcagtcaaaaagggtctgcctcatgctcccgcattgatcaaaagaac
caaccggcggattcccgagagaacttcccatcgagtcgcggatcacgcgcaagtggtccgcgtgctcggattcttccag
tgtcactcccaccccgcacaagcgttcgatgacgccatgactcaatttggtatgtcgagacacggactgctacagctct
ttcgtagagtcggtgtcacagaactcgaggcccgctcgggcacactgcctcccgcctcccagcggtgggacaggattct
ccaagcgagcggtatgaaacgcgcgaagccttcacctacgtcaactcagacacctgaccaggcgagccttcatgcgttc
gcagactcgctggagagggatttggacgcgccctcgcccatgcatgaaggggaccaaactcgcgcgtcagctagcccca
agaagaagagaaaggtgggcagtggtgctcatattgtcatggttgatgcttacaagccaactaaggagctctgagcatg
tgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagca
taaattactatacttctattactagagcccgccgccaccatggag