##gff-version 3
##sequence-region BBa_K1861070 1 2657
BBa_K1861070	.	terminator	2529	2657	.	+	0	ID=BBa_K392003;Name=BBa_K392003
BBa_K1861070	.	CDS	266	2480	.	+	0	ID=BBa_K1861701;Name=BBa_K1861701
BBa_K1861070	.	CDS	266	724	.	+	0	ID=annotation2476635;Name=Front constant domain;Parent=BBa_K1861701
BBa_K1861070	.	CDS	725	1960	.	+	0	ID=annotation2476636;Name=Variable domain;Parent=BBa_K1861701
BBa_K1861070	.	start_codon	266	268	.	+	0	ID=annotation2476634;Name=start codon ATG;Parent=BBa_K1861701
BBa_K1861070	.	CDS	1961	2476	.	+	0	ID=annotation2476637;Name=Back constant domain;Parent=BBa_K1861701
BBa_K1861070	.	sequence_feature	2520	2528	.	+	0	ID=BBa_K1861104;Name=BBa_K1861104
BBa_K1861070	.	sequence_feature	2520	2525	.	+	0	ID=annotation2476131;Name=SacI restriction site;Parent=BBa_K1861104
BBa_K1861070	.	stop_codon	2526	2528	.	+	0	ID=annotation2476132;Name=TGA stop codon;Parent=BBa_K1861104
BBa_K1861070	.	tag	239	265	.	+	0	ID=BBa_K1861102;Name=BBa_K1861102
BBa_K1861070	.	sequence_feature	260	265	.	+	0	ID=annotation2476068;Name=BamHI restriction site;Parent=BBa_K1861102
BBa_K1861070	.	start_codon	239	241	.	+	0	ID=annotation2476066;Name=ATG start codon;Parent=BBa_K1861102
BBa_K1861070	.	tag	242	259	.	+	0	ID=annotation2476067;Name=6x His-Tag;Parent=BBa_K1861102
BBa_K1861070	.	promoter	1	238	.	+	0	ID=BBa_K1861101;Name=BBa_K1861101
BBa_K1861070	.	promoter	1	238	.	+	0	ID=annotation2476069;Name=rpd3 promoter;Parent=BBa_K1861101
BBa_K1861070	.	sequence_alteration	133	133	.	+	0	ID=annotation2476062;Name=T->C mutation to avoid SpeI restriction site;Parent=BBa_K1861101
BBa_K1861070	.	CDS	2481	2519	.	+	0	ID=BBa_K1159201;Name=BBa_K1159201
BBa_K1861070	.	conserved_region	2499	2501	.	+	0	ID=annotation2343512;Name=Reactive Asp for isopeptide forming reaction;Parent=BBa_K1159201
BBa_K1861070	.	tag	2481	2519	.	+	0	ID=annotation2343481;Name=SpyTag;Parent=BBa_K1159201
>BBa_K1861070
gtaatatcaactcagagcgtataggtaaatttgtaaatatatgtcccatattttgccttgaaatttatctttttattta
cttaccctccccggctcatagtatctatcgaaagggaaaaacagaaaagataccagtagttgattatacataataaaaa
tttgaagtaataaccataaaggttcataaaacaattgcgccatacaaaacattcgtggctacaactcgatatccgtgca
gatgcatcaccaccatcatcacggatccatggcccccaagaagaagaggaaggtgggcattcaccgcggggtacctatg
gtggacttgaggacactcggttattcgcaacagcaacaggagaaaatcaagcctaaggtcaggagcaccgtcgcgcaac
accacgaggcgcttgtggggcatggcttcactcatgcgcatattgtcgcgctttcacagcaccctgcggcgcttgggac
ggtggctgtcaaataccaagatatgattgcggccctgcccgaagccacgcacgaggcaattgtaggggtcggtaaacag
tggtcgggagcgcgagcacttgaggcgctgctgactgtggcgggtgagcttagggggcctccgctccagctcgacaccg
ggcagctgctgaagatcgcgaagagagggggagtaacagcggtagaggcagtgcacgcctggcgcaatgcgctcaccgg
tgcccccctccctaaatttgacgccggaccaggtcgtagcgatagcttcccatgacgggggaaaacaagcgctggaaac
agtacaaagattgctgccggtcctttgtcaagaccacggccttactccggatcaagttgtcgcaatcgcatctcacgat
gggggtaaacaggcattagagacagttcaaagactactgcccgtcttgtgtcaggatcacggtctaactcccgatcaag
tagttgccatagcgtctaaccacggaggcaaacaggcgcttgagactgttcagcgtcttctaccagtgctttgccagga
ccatgggctgacacccgaccaggttgtggccattgctagtaatataggcgggaagcaggcgttggagactgttcaacgt
ttattacccgtactatgccaagatcatggcttgactcctgaccaagtggtcgccatcgcttctcatgatggaggaaagc
aagcattggaaactgtccaaaggcttcttccggtcttatgtcaggatcacggattgacaccagatcaggtggttgctat
tgcctctaacggcggtggtaagcaagccctagaaaccgtccagagattgttgccggtattcaagaccacgggttgactc
ctgatcaagtagtcgccatagctagccatgacgggggcaagcaagctttagaaacagttcagaggttgctgccagtctt
atgtcaggatcacggtttgaccccggaccaggtggtcgctattgcatctaatcacgggggtaaacaagcactagaaact
gtacaaaggttgttgccggtattatgccaggaccatggacttactcccgatcaagttgtggccatcgcatctcatgatg
gtgggaaacaggctttggaaaccgttcaacgtcttttaccagtgttgtgccaagatcatgggctaacacccgaccaagt
ggtagcgattgcctctaatggtggcggcaaacaggccctggaaacggttcagcgtttgttacccgtactgtgtcaggat
cacggattaacgccagaccaggttgtagctatcgcttctaaccatggcggtaagcaggcgctagagacagtccaaagac
tgctgccggttctttgtcaagaccacggtcttaccccagatcaggtcgttgccattgcgtctaacggaggtggaaagca
agcattggagtctatcgttgctcaattatccagaccagatccagcacttgctgctttgactaacgaacgaccatctggt
ggcgttggcatgtcttggtggacgacccgcgctcgatgcagtcaaaaagggtctgcctcatgctcccgcattgatcaaa
agaaccaaccggcggattcccgagagaacttcccatcgagtcgcggatcacgcgcaagtggtccgcgtgctcggattct
tccagtgtcactcccaccccgcacaagcgttcgatgacgccatgactcaatttggtatgtcgagacacggactgctaca
gctctttcgtagagtcggtgtcacagaactcgaggcccgctcgggcacactgcctcccgcctcccagcggtgggacagg
attctccaagcgagcggtatgaaacgcgcgaagccttcacctacgtcaactcagacacctgaccaggcgagccttcatg
cgttcgcagactcgctggagagggatttggacgcgccctcgcccatgcatgaaggggaccaaactcgcgcgtcagctag
ccccaagaagaagagaaaggtgggcagtggtgctcatattgtcatggttgatgcttacaagccaactaaggagctctga
gcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttg
cagcataaattactatacttctattactagagcccgccgccaccatggag