BBa_K1861701 1 TALE3 TALE that binds the sequence T CCGACTCGCTGT 2015-09-17T11:00:00Z 2015-09-23T04:18:26Z Coming soon. Coming soon. false false _2291_ 25348 25348 9 false Coming soon. false Vladyslav Vyshnevskyi annotation2476635 1 Front constant domain range2476635 1 1 459 annotation2476636 1 Variable domain range2476636 1 460 1695 annotation2476637 1 Back constant domain range2476637 1 1696 2211 annotation2476634 1 start codon ATG range2476634 1 1 3 BBa_K1861101 1 BBa_K1861101 rpd3 promoter 2015-09-17T11:00:00Z 2015-09-19T09:23:03Z Coming soon. Coming soon. false false _2291_ 25348 25348 9 false Coming soon. false Vladyslav Vyshnevskyi annotation2476062 1 T->C mutation to avoid SpeI restriction site range2476062 1 133 133 annotation2476069 1 rpd3 promoter range2476069 1 1 238 BBa_K1861104 1 BBa_K1861104 SacI restriction site and stop codon 2015-09-17T11:00:00Z 2015-09-19T02:45:11Z Coming soon. Coming soon. false false _2291_ 25348 25348 9 false Coming soon. false Vladyslav Vyshnevskyi annotation2476131 1 SacI restriction site range2476131 1 1 6 annotation2476132 1 TGA stop codon range2476132 1 7 9 BBa_K392003 1 BBa_K392003 yeast ADH1 terminator 2010-10-09T11:00:00Z 2015-05-08T01:12:20Z Registry parts BBa_K105027 (promoter) and BBa_J63003 (Kozak). Engineered 'cyc100 promoter' of <i>Saccharomyces cerevisiae</i> with Kozak sequence attached downstream. false false _528_ 0 6220 9 It's complicated true Assembled by 3A method. false Tadashi Nakamura, Shuhei Yasumoto, Takahiro Saka, Saya Kakuda BBa_K1861070 1 BBa_K1861070 Fusion protein of TALE3 and a SpyTag in an expression cassette 2015-09-17T11:00:00Z 2015-09-23T03:37:16Z Coming soon. Coming soon. false false _2291_ 25348 25348 9 false Coming soon. false Vladyslav Vyshnevskyi component2474670 1 BBa_K1861701 component2474668 1 BBa_K1861101 component2474675 1 BBa_K392003 component2474673 1 BBa_K1159201 component2474669 1 BBa_K1861102 component2474674 1 BBa_K1861104 annotation2474673 1 BBa_K1159201 range2474673 1 2481 2519 annotation2474670 1 BBa_K1861701 range2474670 1 266 2480 annotation2474675 1 BBa_K392003 range2474675 1 2529 2657 annotation2474674 1 BBa_K1861104 range2474674 1 2520 2528 annotation2474668 1 BBa_K1861101 range2474668 1 1 238 annotation2474669 1 BBa_K1861102 range2474669 1 239 265 BBa_K1159201 1 BBa_K1159201 Splitted and engineered C-terminal FbaB for isopeptide bound formation (SpyTag) in RFC[25] 2013-09-13T11:00:00Z 2015-05-08T01:09:31Z ''Streptococcus pyogenes'' This part codes for a oligopeptide that is recognized and bound by a covalent isopeptide bound to a protein, called SpyCatcher. That catcher protein for this oligopeptdie can be found under BBa_K1159200. This part is flanked by RFC[25] pre- and suffix for further protein fusions. false false _1471_ 0 11507 9 In stock false x false Dong-Jiunn Jeffery TRUONG annotation2343481 1 SpyTag range2343481 1 1 39 annotation2343512 1 Reactive Asp for isopeptide forming reaction range2343512 1 19 21 BBa_K1861102 1 BBa_K1861102 His-Tag 2015-09-17T11:00:00Z 2015-09-19T09:19:53Z Coming soon. Coming soon. false false _2291_ 25348 25348 9 false Coming soon. false Vladyslav Vyshnevskyi annotation2476067 1 6x His-Tag range2476067 1 4 21 annotation2476068 1 BamHI restriction site range2476068 1 22 27 annotation2476066 1 ATG start codon range2476066 1 1 3 BBa_K1861070_sequence 1 gtaatatcaactcagagcgtataggtaaatttgtaaatatatgtcccatattttgccttgaaatttatctttttatttacttaccctccccggctcatagtatctatcgaaagggaaaaacagaaaagataccagtagttgattatacataataaaaatttgaagtaataaccataaaggttcataaaacaattgcgccatacaaaacattcgtggctacaactcgatatccgtgcagatgcatcaccaccatcatcacggatccatggcccccaagaagaagaggaaggtgggcattcaccgcggggtacctatggtggacttgaggacactcggttattcgcaacagcaacaggagaaaatcaagcctaaggtcaggagcaccgtcgcgcaacaccacgaggcgcttgtggggcatggcttcactcatgcgcatattgtcgcgctttcacagcaccctgcggcgcttgggacggtggctgtcaaataccaagatatgattgcggccctgcccgaagccacgcacgaggcaattgtaggggtcggtaaacagtggtcgggagcgcgagcacttgaggcgctgctgactgtggcgggtgagcttagggggcctccgctccagctcgacaccgggcagctgctgaagatcgcgaagagagggggagtaacagcggtagaggcagtgcacgcctggcgcaatgcgctcaccggtgcccccctccctaaatttgacgccggaccaggtcgtagcgatagcttcccatgacgggggaaaacaagcgctggaaacagtacaaagattgctgccggtcctttgtcaagaccacggccttactccggatcaagttgtcgcaatcgcatctcacgatgggggtaaacaggcattagagacagttcaaagactactgcccgtcttgtgtcaggatcacggtctaactcccgatcaagtagttgccatagcgtctaaccacggaggcaaacaggcgcttgagactgttcagcgtcttctaccagtgctttgccaggaccatgggctgacacccgaccaggttgtggccattgctagtaatataggcgggaagcaggcgttggagactgttcaacgtttattacccgtactatgccaagatcatggcttgactcctgaccaagtggtcgccatcgcttctcatgatggaggaaagcaagcattggaaactgtccaaaggcttcttccggtcttatgtcaggatcacggattgacaccagatcaggtggttgctattgcctctaacggcggtggtaagcaagccctagaaaccgtccagagattgttgccggtattcaagaccacgggttgactcctgatcaagtagtcgccatagctagccatgacgggggcaagcaagctttagaaacagttcagaggttgctgccagtcttatgtcaggatcacggtttgaccccggaccaggtggtcgctattgcatctaatcacgggggtaaacaagcactagaaactgtacaaaggttgttgccggtattatgccaggaccatggacttactcccgatcaagttgtggccatcgcatctcatgatggtgggaaacaggctttggaaaccgttcaacgtcttttaccagtgttgtgccaagatcatgggctaacacccgaccaagtggtagcgattgcctctaatggtggcggcaaacaggccctggaaacggttcagcgtttgttacccgtactgtgtcaggatcacggattaacgccagaccaggttgtagctatcgcttctaaccatggcggtaagcaggcgctagagacagtccaaagactgctgccggttctttgtcaagaccacggtcttaccccagatcaggtcgttgccattgcgtctaacggaggtggaaagcaagcattggagtctatcgttgctcaattatccagaccagatccagcacttgctgctttgactaacgaacgaccatctggtggcgttggcatgtcttggtggacgacccgcgctcgatgcagtcaaaaagggtctgcctcatgctcccgcattgatcaaaagaaccaaccggcggattcccgagagaacttcccatcgagtcgcggatcacgcgcaagtggtccgcgtgctcggattcttccagtgtcactcccaccccgcacaagcgttcgatgacgccatgactcaatttggtatgtcgagacacggactgctacagctctttcgtagagtcggtgtcacagaactcgaggcccgctcgggcacactgcctcccgcctcccagcggtgggacaggattctccaagcgagcggtatgaaacgcgcgaagccttcacctacgtcaactcagacacctgaccaggcgagccttcatgcgttcgcagactcgctggagagggatttggacgcgccctcgcccatgcatgaaggggaccaaactcgcgcgtcagctagccccaagaagaagagaaaggtgggcagtggtgctcatattgtcatggttgatgcttacaagccaactaaggagctctgagcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctattactagagcccgccgccaccatggag BBa_K1861102_sequence 1 atgcatcaccaccatcatcacggatcc BBa_K1861101_sequence 1 gtaatatcaactcagagcgtataggtaaatttgtaaatatatgtcccatattttgccttgaaatttatctttttatttacttaccctccccggctcatagtatctatcgaaagggaaaaacagaaaagataccagtagttgattatacataataaaaatttgaagtaataaccataaaggttcataaaacaattgcgccatacaaaacattcgtggctacaactcgatatccgtgcag BBa_K392003_sequence 1 gcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctattactagagcccgccgccaccatggag BBa_K1861104_sequence 1 gagctctga BBa_K1159201_sequence 1 gctcatattgtcatggttgatgcttacaagccaactaag BBa_K1861701_sequence 1 atggcccccaagaagaagaggaaggtgggcattcaccgcggggtacctatggtggacttgaggacactcggttattcgcaacagcaacaggagaaaatcaagcctaaggtcaggagcaccgtcgcgcaacaccacgaggcgcttgtggggcatggcttcactcatgcgcatattgtcgcgctttcacagcaccctgcggcgcttgggacggtggctgtcaaataccaagatatgattgcggccctgcccgaagccacgcacgaggcaattgtaggggtcggtaaacagtggtcgggagcgcgagcacttgaggcgctgctgactgtggcgggtgagcttagggggcctccgctccagctcgacaccgggcagctgctgaagatcgcgaagagagggggagtaacagcggtagaggcagtgcacgcctggcgcaatgcgctcaccggtgcccccctaaatttgacgccggaccaggtcgtagcgatagcttcccatgacgggggaaaacaagcgctggaaacagtacaaagattgctgccggtcctttgtcaagaccacggccttactccggatcaagttgtcgcaatcgcatctcacgatgggggtaaacaggcattagagacagttcaaagactactgcccgtcttgtgtcaggatcacggtctaactcccgatcaagtagttgccatagcgtctaaccacggaggcaaacaggcgcttgagactgttcagcgtcttctaccagtgctttgccaggaccatgggctgacacccgaccaggttgtggccattgctagtaatataggcgggaagcaggcgttggagactgttcaacgtttattacccgtactatgccaagatcatggcttgactcctgaccaagtggtcgccatcgcttctcatgatggaggaaagcaagcattggaaactgtccaaaggcttcttccggtcttatgtcaggatcacggattgacaccagatcaggtggttgctattgcctctaacggcggtggtaagcaagccctagaaaccgtccagagattgttgccggtattgtgtcaagaccacgggttgactcctgatcaagtagtcgccatagctagccatgacgggggcaagcaagctttagaaacagttcagaggttgctgccagtcttatgtcaggatcacggtttgaccccggaccaggtggtcgctattgcatctaatcacgggggtaaacaagcactagaaactgtacaaaggttgttgccggtattatgccaggaccatggacttactcccgatcaagttgtggccatcgcatctcatgatggtgggaaacaggctttggaaaccgttcaacgtcttttaccagtgttgtgccaagatcatgggctaacacccgaccaagtggtagcgattgcctctaatggtggcggcaaacaggccctggaaacggttcagcgtttgttacccgtactgtgtcaggatcacggattaacgccagaccaggttgtagctatcgcttctaaccatggcggtaagcaggcgctagagacagtccaaagactgctgccggttctttgtcaagaccacggtcttaccccagatcaggtcgttgccattgcgtctaacggaggtggaaagcaagcattggagtctatcgttgctcaattatccagaccagatccagcacttgctgctttgactaacgaccatctggtggcgttggcatgtcttggtggacgacccgcgctcgatgcagtcaaaaagggtctgcctcatgctcccgcattgatcaaaagaaccaaccggcggattcccgagagaacttcccatcgagtcgcggatcacgcgcaagtggtccgcgtgctcggattcttccagtgtcactcccaccccgcacaagcgttcgatgacgccatgactcaatttggtatgtcgagacacggactgctacagctctttcgtagagtcggtgtcacagaactcgaggcccgctcgggcacactgcctcccgcctcccagcggtgggacaggattctccaagcgagcggtatgaaacgcgcgaagccttcacctacgtcaactcagacacctgaccaggcgagccttcatgcgttcgcagactcgctggagagggatttggacgcgccctcgcccatgcatgaaggggaccaaactcgcgcgtcagctagccccaagaagaagagaaaggtgggcagtggt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z