BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K880005 1 BBa_K880005 Strong promoter, strong RBS combination for high expression levels of proteins 2012-09-28T11:00:00Z 2015-05-08T01:13:40Z It is a combination of strong promoter (J23100) and RBS (B0034). Released HQ 2013 -J23100+B0034 -Strong promoter, strong RBS combination for high expression levels of proteins Consensus constitutive promoter and RBS sequence-produces strongest possible expression. false false _1142_ 0 9403 9 In stock false Enter design considerations. false Josh Atkinson, Mike Ferguson, and Ben Parker component2204228 1 BBa_J23100 component2204230 1 BBa_B0034 annotation2204230 1 BBa_B0034 range2204230 1 44 55 annotation2204228 1 BBa_J23100 range2204228 1 1 35 BBa_K1866004 1 BBa_K1866004 Promoter+RibosomeBindingSite+NosZ+SuperYellowFluorescenceProtein (in pSB1A3) 2015-09-15T11:00:00Z 2015-09-18T04:54:18Z The Promoter+RibosomeBindingSite part was obtained from the biobrick BBa_K880005. The NosZ+SYFP construct was used from the biobrick BBa_K1866001. This part catalyses the conversion of Nitrous Oxide to Nitrogen gas. This part consists of a Super Yellow Fluorescence Protein tag to check the expression of the NosZ gene. false false _2296_ 28697 28697 9 false The NosZ gene has a PstI enzyme site within its sequence. This is why the part is not 3A assembly compatible. We used an alternate strategy to ligate the Promoter+RibosomeBindingSite construct upstream to the NosZ+SYFP construct in its native backbone. false Stefan Oehler component2459066 1 BBa_K1866001 component2459064 1 BBa_K880005 annotation2459064 1 BBa_K880005 range2459064 1 1 55 annotation2459066 1 BBa_K1866001 range2459066 1 62 1972 BBa_K1866001 1 NosZ+SYFP NosZ gene+SuperYellowFluorescenceProtein (in pSB1A3) 2015-09-15T11:00:00Z 2015-09-18T04:52:30Z The gene NosZ was isolated from Pseudomonas aeruginosa genome. (BBa_K1356006) SYFP sequence was obtained from the distribution kit. This part consists of NosZ gene and a sequence encoding for Super Yellow Fluorescence Protein downstream to it. NosZ gene product catalyses the conversion of Nitrous Oxide (N2O) to molecular Nitrogen gas in a process called denitrification. This part can be cloned under the control of a promoter of choice and its expression can be readily analyzed by checking for the expression of the yellow fluorescence protein. false false _2296_ 28697 28697 9 false None. This part is compatible with the 3A assembly. false Stefan Oehler component2458567 1 BBa_K1356006 annotation2458567 1 BBa_K1356006 range2458567 1 1 1911 BBa_K1356006 1 nosZ Nitrous oxide reductase (nosZ) from Pseudomonas aeruginosa PAO1 2014-10-07T11:00:00Z 2015-05-08T01:10:02Z P. L false false _1732_ 0 20075 9 It's complicated false Mutation- fail! false Cameron Sargent BBa_J23100 1 BBa_J23100 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Replace later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_J23100_sequence 1 ttgacggctagctcagtcctaggtacagtgctagc BBa_K1866001_sequence 1 atgagcgacgacacgaaaagcccccacgaagaaacccacggcctgaaccgccgcggcttcctcggcgcctcggcgctgaccggagccgccgccctggttggcgccagcgccctgggcagcgcggtggtcggccgcgaggcccgggccgcgggcaagggcgagcgcagcaaggccgaggtcgcccccggcgaactggatgagtactacgggttctggagcggcggacattccggcgaagtacgcgtgctcggcgtgccgtcgatgcgcgagctgatgcgcataccggtgttcaacgtcgactcggccaccggctggggcctgaccaacgagagcaagcgggtcctcggcgacagcgcgcgcttcctcaacggcgactgccaccatccgcacatctcgatgaccgacggcaagtacgacggcaagtacctgttcatcaacgacaaggccaacagccgggtcgcgcgcatccgcctggacgtcatgaaatgcgaccgcatcgtcaccattcccaacgtccaggcgatccacggcctgcgcctgcaaaaggtgccgcatacccgctacgtgttctgcaacgccgagttcatcatcccccatcccaacgacggctcgaccttcgacctgtccggcgacaacgccttcaccctgtacaacgccatcgacgccgagaccatggaagtggcctggcaggtgatcgtcgacggcaacctcgacaacaccgacatggactacagcggcaggttcgccgcctccacctgctacaactcggagaaggccgtcgacctcggcggcatgatgcgcaacgagcgcgactgggtggtggtgttcgacatcccgcgcatcgaggccgagatcaaggcgaagcgcttcgtcaccctcggcgactcgaaggtgccggtggtcgacggccggcgcaaggacggcaaggacagcccggtgacccgctacatcccggtaccgaagaacccccacgggctgaacacctcgccggacggcaagtacttcatcgccaacggcaagctctcgccgacctgcaccatgatcgccatcgagcgcctcggcgacctgttcgccggcaagctggccgacccgcgcgacgtggtggtgggcgagccggaactgggcctcggcccgctgcacaccactttcgatggccgaggcaacgcctataccacgctgttcatcgacagccagttggtgaagtggaacctggccgacgcggtgcgcgcctacaagggcgagaaggtcgactacatccgccagaaactcgacgtgcagtaccagccggggcacaaccacgccaccctgtgcgagaccagcgaagccgacggcaagtggatcgtggtgctcagcaagttctccaaggaccgcttcctgcccaccggtccgctgcacccggagaacgaccagttgatcgacatttccggcgaggaaatgaagctggtccacgacggcccgaccttcgccgaaccgcacgattgcatcctcgcccgccgcgaccagatcaagacccgcaagatctgggaccgcaaggacccgttcttcgccgagacggtcaagcgcgcggaaaaggacggcatcgacctgatgaaggacaacaaggtcatccgcgagggcaacaaggtccgcgtctacatggtctcgatggcgccctccttcggcctcaccgagttcaaggtgaagcagggcgacgaagtcaccgtgaccatcaccaacctcgacgagatcgaggacgtgacccacggcttcgtcatggtcaaccacggcgtctgcatggagatcagcccgcaacagacctcgtcgatcaccttcgtcgccgataagcccggggtgcactggtactactgcagctggttctgccacgccctgcacatggaaatgtgcgggcggatgctggtggaaaaggcttga BBa_B0034_sequence 1 aaagaggagaaa BBa_K1356006_sequence 1 atgagcgacgacacgaaaagcccccacgaagaaacccacggcctgaaccgccgcggcttcctcggcgcctcggcgctgaccggagccgccgccctggttggcgccagcgccctgggcagcgcggtggtcggccgcgaggcccgggccgcgggcaagggcgagcgcagcaaggccgaggtcgcccccggcgaactggatgagtactacgggttctggagcggcggacattccggcgaagtacgcgtgctcggcgtgccgtcgatgcgcgagctgatgcgcataccggtgttcaacgtcgactcggccaccggctggggcctgaccaacgagagcaagcgggtcctcggcgacagcgcgcgcttcctcaacggcgactgccaccatccgcacatctcgatgaccgacggcaagtacgacggcaagtacctgttcatcaacgacaaggccaacagccgggtcgcgcgcatccgcctggacgtcatgaaatgcgaccgcatcgtcaccattcccaacgtccaggcgatccacggcctgcgcctgcaaaaggtgccgcatacccgctacgtgttctgcaacgccgagttcatcatcccccatcccaacgacggctcgaccttcgacctgtccggcgacaacgccttcaccctgtacaacgccatcgacgccgagaccatggaagtggcctggcaggtgatcgtcgacggcaacctcgacaacaccgacatggactacagcggcaggttcgccgcctccacctgctacaactcggagaaggccgtcgacctcggcggcatgatgcgcaacgagcgcgactgggtggtggtgttcgacatcccgcgcatcgaggccgagatcaaggcgaagcgcttcgtcaccctcggcgactcgaaggtgccggtggtcgacggccggcgcaaggacggcaaggacagcccggtgacccgctacatcccggtaccgaagaacccccacgggctgaacacctcgccggacggcaagtacttcatcgccaacggcaagctctcgccgacctgcaccatgatcgccatcgagcgcctcggcgacctgttcgccggcaagctggccgacccgcgcgacgtggtggtgggcgagccggaactgggcctcggcccgctgcacaccactttcgatggccgaggcaacgcctataccacgctgttcatcgacagccagttggtgaagtggaacctggccgacgcggtgcgcgcctacaagggcgagaaggtcgactacatccgccagaaactcgacgtgcagtaccagccggggcacaaccacgccaccctgtgcgagaccagcgaagccgacggcaagtggatcgtggtgctcagcaagttctccaaggaccgcttcctgcccaccggtccgctgcacccggagaacgaccagttgatcgacatttccggcgaggaaatgaagctggtccacgacggcccgaccttcgccgaaccgcacgattgcatcctcgcccgccgcgaccagatcaagacccgcaagatctgggaccgcaaggacccgttcttcgccgagacggtcaagcgcgcggaaaaggacggcatcgacctgatgaaggacaacaaggtcatccgcgagggcaacaaggtccgcgtctacatggtctcgatggcgccctccttcggcctcaccgagttcaaggtgaagcagggcgacgaagtcaccgtgaccatcaccaacctcgacgagatcgaggacgtgacccacggcttcgtcatggtcaaccacggcgtctgcatggagatcagcccgcaacagacctcgtcgatcaccttcgtcgccgataagcccggggtgcactggtactactgcagctggttctgccacgccctgcacatggaaatgtgcgggcggatgctggtggaaaaggcttga BBa_K1866004_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgagcgacgacacgaaaagcccccacgaagaaacccacggcctgaaccgccgcggcttcctcggcgcctcggcgctgaccggagccgccgccctggttggcgccagcgccctgggcagcgcggtggtcggccgcgaggcccgggccgcgggcaagggcgagcgcagcaaggccgaggtcgcccccggcgaactggatgagtactacgggttctggagcggcggacattccggcgaagtacgcgtgctcggcgtgccgtcgatgcgcgagctgatgcgcataccggtgttcaacgtcgactcggccaccggctggggcctgaccaacgagagcaagcgggtcctcggcgacagcgcgcgcttcctcaacggcgactgccaccatccgcacatctcgatgaccgacggcaagtacgacggcaagtacctgttcatcaacgacaaggccaacagccgggtcgcgcgcatccgcctggacgtcatgaaatgcgaccgcatcgtcaccattcccaacgtccaggcgatccacggcctgcgcctgcaaaaggtgccgcatacccgctacgtgttctgcaacgccgagttcatcatcccccatcccaacgacggctcgaccttcgacctgtccggcgacaacgccttcaccctgtacaacgccatcgacgccgagaccatggaagtggcctggcaggtgatcgtcgacggcaacctcgacaacaccgacatggactacagcggcaggttcgccgcctccacctgctacaactcggagaaggccgtcgacctcggcggcatgatgcgcaacgagcgcgactgggtggtggtgttcgacatcccgcgcatcgaggccgagatcaaggcgaagcgcttcgtcaccctcggcgactcgaaggtgccggtggtcgacggccggcgcaaggacggcaaggacagcccggtgacccgctacatcccggtaccgaagaacccccacgggctgaacacctcgccggacggcaagtacttcatcgccaacggcaagctctcgccgacctgcaccatgatcgccatcgagcgcctcggcgacctgttcgccggcaagctggccgacccgcgcgacgtggtggtgggcgagccggaactgggcctcggcccgctgcacaccactttcgatggccgaggcaacgcctataccacgctgttcatcgacagccagttggtgaagtggaacctggccgacgcggtgcgcgcctacaagggcgagaaggtcgactacatccgccagaaactcgacgtgcagtaccagccggggcacaaccacgccaccctgtgcgagaccagcgaagccgacggcaagtggatcgtggtgctcagcaagttctccaaggaccgcttcctgcccaccggtccgctgcacccggagaacgaccagttgatcgacatttccggcgaggaaatgaagctggtccacgacggcccgaccttcgccgaaccgcacgattgcatcctcgcccgccgcgaccagatcaagacccgcaagatctgggaccgcaaggacccgttcttcgccgagacggtcaagcgcgcggaaaaggacggcatcgacctgatgaaggacaacaaggtcatccgcgagggcaacaaggtccgcgtctacatggtctcgatggcgccctccttcggcctcaccgagttcaaggtgaagcagggcgacgaagtcaccgtgaccatcaccaacctcgacgagatcgaggacgtgacccacggcttcgtcatggtcaaccacggcgtctgcatggagatcagcccgcaacagacctcgtcgatcaccttcgtcgccgataagcccggggtgcactggtactactgcagctggttctgccacgccctgcacatggaaatgtgcgggcggatgctggtggaaaaggcttga BBa_K880005_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z