BBa_K864101 1 BBa_K864101 B0032-SYFP2 2012-09-24T11:00:00Z 2015-05-08T01:13:37Z Assembly of K864100 and the B0032 from the registry. Released HQ 2013 This part is the <partinfo short>BBa_K864100</partinfo> (<partinfo>BBa_K864100</partinfo>) with the medium strength <partinfo>BBa_B0032</partinfo> RBS. The B0032 RBS is commonly used for promoter testing, giving a wider dynamic range than a stronger RBS. false false _1124_ 0 13993 9 In stock false Transcriptional leakage is present in some backbones, giving visibly fluorescent colonies even without promoter. false Arvid Hed??n Gynn?? component2197145 1 BBa_B0032 component2197148 1 BBa_K864100 annotation2197145 1 BBa_B0032 range2197145 1 1 13 annotation2197148 1 BBa_K864100 range2197148 1 20 742 BBa_B0032 1 BBa_B0032 RBS.3 (medium) -- derivative of BBa_0030 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _41_44_48_46_1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;RBS-2&quot; in figure 4-14 of thesis). <P> Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation7027 1 BBa_B0032 range7027 1 1 13 annotation1709 1 RBS-3\Weak range1709 1 1 13 annotation1710 1 RBS range1710 1 7 10 BBa_K1866007 1 BBa_K1866007 NosZ+SuperYellowFluorescenceProtein (in pSB1C3) 2015-09-17T11:00:00Z 2015-09-18T05:23:55Z It was obtained from Pseudomonas genome. This part consists of NosZ gene and a sequence encoding for Super Yellow Fluorescence Protein downstream to it. NosZ gene product catalyses the conversion of Nitrous Oxide (N2O) to molecular Nitrogen gas in a process called denitrification. This part can be cloned under the control of a promoter of choice and its expression can be readily analyzed by checking for the expression of the yellow fluorescence protein. false false _2296_ 28697 28697 9 false None. It is 3A compatible. false Stefan Oehler component2474274 1 BBa_K1356006 component2474280 1 BBa_K864101 annotation2474280 1 BBa_K864101 range2474280 1 1920 2661 annotation2474274 1 BBa_K1356006 range2474274 1 1 1911 BBa_K864100 1 SYFP2 Super Yellow Fluorescent Protein 2 (SYFP2) 2012-09-23T11:00:00Z 2016-01-21T02:29:24Z Amino acid sequence taken from the article by Kremers et al 2006. Released HQ 2013 This part codes for the bright yellow fluorescent protein SYFP2. SYFP2 is a GFP based monomeric protein with a narrow fluorescence emission spectrum with a maximum at 515 nm. Bacteria expressing SYFP2 are reported to be 12 times brighter than those expressing EYFP(Q69K) and almost 2-fold brighter than bacteria expressing Venus (Ref. 10.1021/bi0516273). Codon optimized for expression in E coli by DNA 2.0. Mutations compared to wtGFP amino acid sequence (GenBank Accession number M62653) are F46L F64L S65G S72A M153T V163A S175G T203Y A206K. false false _1124_ 4206 10137 9 In stock false The sequence has been codon optimized for expression in E coli by DNA 2.0. false Erik Gullberg annotation2196221 1 SYFP2 range2196221 1 1 717 BBa_K1356006 1 nosZ Nitrous oxide reductase (nosZ) from Pseudomonas aeruginosa PAO1 2014-10-07T11:00:00Z 2015-05-08T01:10:02Z P. L false false _1732_ 0 20075 9 It's complicated false Mutation- fail! false Cameron Sargent BBa_K1866007_sequence 1 atgagcgacgacacgaaaagcccccacgaagaaacccacggcctgaaccgccgcggcttcctcggcgcctcggcgctgaccggagccgccgccctggttggcgccagcgccctgggcagcgcggtggtcggccgcgaggcccgggccgcgggcaagggcgagcgcagcaaggccgaggtcgcccccggcgaactggatgagtactacgggttctggagcggcggacattccggcgaagtacgcgtgctcggcgtgccgtcgatgcgcgagctgatgcgcataccggtgttcaacgtcgactcggccaccggctggggcctgaccaacgagagcaagcgggtcctcggcgacagcgcgcgcttcctcaacggcgactgccaccatccgcacatctcgatgaccgacggcaagtacgacggcaagtacctgttcatcaacgacaaggccaacagccgggtcgcgcgcatccgcctggacgtcatgaaatgcgaccgcatcgtcaccattcccaacgtccaggcgatccacggcctgcgcctgcaaaaggtgccgcatacccgctacgtgttctgcaacgccgagttcatcatcccccatcccaacgacggctcgaccttcgacctgtccggcgacaacgccttcaccctgtacaacgccatcgacgccgagaccatggaagtggcctggcaggtgatcgtcgacggcaacctcgacaacaccgacatggactacagcggcaggttcgccgcctccacctgctacaactcggagaaggccgtcgacctcggcggcatgatgcgcaacgagcgcgactgggtggtggtgttcgacatcccgcgcatcgaggccgagatcaaggcgaagcgcttcgtcaccctcggcgactcgaaggtgccggtggtcgacggccggcgcaaggacggcaaggacagcccggtgacccgctacatcccggtaccgaagaacccccacgggctgaacacctcgccggacggcaagtacttcatcgccaacggcaagctctcgccgacctgcaccatgatcgccatcgagcgcctcggcgacctgttcgccggcaagctggccgacccgcgcgacgtggtggtgggcgagccggaactgggcctcggcccgctgcacaccactttcgatggccgaggcaacgcctataccacgctgttcatcgacagccagttggtgaagtggaacctggccgacgcggtgcgcgcctacaagggcgagaaggtcgactacatccgccagaaactcgacgtgcagtaccagccggggcacaaccacgccaccctgtgcgagaccagcgaagccgacggcaagtggatcgtggtgctcagcaagttctccaaggaccgcttcctgcccaccggtccgctgcacccggagaacgaccagttgatcgacatttccggcgaggaaatgaagctggtccacgacggcccgaccttcgccgaaccgcacgattgcatcctcgcccgccgcgaccagatcaagacccgcaagatctgggaccgcaaggacccgttcttcgccgagacggtcaagcgcgcggaaaaggacggcatcgacctgatgaaggacaacaaggtcatccgcgagggcaacaaggtccgcgtctacatggtctcgatggcgccctccttcggcctcaccgagttcaaggtgaagcagggcgacgaagtcaccgtgaccatcaccaacctcgacgagatcgaggacgtgacccacggcttcgtcatggtcaaccacggcgtctgcatggagatcagcccgcaacagacctcgtcgatcaccttcgtcgccgataagcccggggtgcactggtactactgcagctggttctgccacgccctgcacatggaaatgtgcgggcggatgctggtggaaaaggcttgatactagagtcacacaggaaagtactagatggttagcaagggcgaagaactttttacaggcgtagtaccgatcttagttgaattagacggcgacgttaacggtcataagtttagcgtgagcggtgagggtgaaggtgacgcaacttacggcaagctgaccctgaagctgatttgcacgacgggtaagctgccggtcccgtggcctaccctggtcacgaccttgggttatggcgttcagtgtttcgcgcgttatccggaccacatgaaacaacacgatttctttaagagcgcgatgccagaaggctatgtgcaggagcgtacgatctttttcaaagacgacggtaactacaagacgcgtgccgaagtcaaattcgaaggcgacaccctggtgaatcgcattgagctgaagggtattgatttcaaagaggatggcaatatcctgggtcacaagctggagtacaattacaattcccacaacgtttacatcaccgcagataaacagaaaaatggcatcaaagcgaatttcaaaatccgtcacaacattgaggacggtggtgttcaactggcggatcattaccagcaaaacaccccgattggtgacggtccggtcctgttgccggataaccattatctgtcttaccaaagcaaactgagcaaagatccgaacgagaagcgcgaccacatggtgctgctggagtttgtgaccgctgccggtattaccctgggtatggatgagctgtataaataataa BBa_K864100_sequence 1 atggttagcaagggcgaagaactttttacaggcgtagtaccgatcttagttgaattagacggcgacgttaacggtcataagtttagcgtgagcggtgagggtgaaggtgacgcaacttacggcaagctgaccctgaagctgatttgcacgacgggtaagctgccggtcccgtggcctaccctggtcacgaccttgggttatggcgttcagtgtttcgcgcgttatccggaccacatgaaacaacacgatttctttaagagcgcgatgccagaaggctatgtgcaggagcgtacgatctttttcaaagacgacggtaactacaagacgcgtgccgaagtcaaattcgaaggcgacaccctggtgaatcgcattgagctgaagggtattgatttcaaagaggatggcaatatcctgggtcacaagctggagtacaattacaattcccacaacgtttacatcaccgcagataaacagaaaaatggcatcaaagcgaatttcaaaatccgtcacaacattgaggacggtggtgttcaactggcggatcattaccagcaaaacaccccgattggtgacggtccggtcctgttgccggataaccattatctgtcttaccaaagcaaactgagcaaagatccgaacgagaagcgcgaccacatggtgctgctggagtttgtgaccgctgccggtattaccctgggtatggatgagctgtataaataataa BBa_K1356006_sequence 1 atgagcgacgacacgaaaagcccccacgaagaaacccacggcctgaaccgccgcggcttcctcggcgcctcggcgctgaccggagccgccgccctggttggcgccagcgccctgggcagcgcggtggtcggccgcgaggcccgggccgcgggcaagggcgagcgcagcaaggccgaggtcgcccccggcgaactggatgagtactacgggttctggagcggcggacattccggcgaagtacgcgtgctcggcgtgccgtcgatgcgcgagctgatgcgcataccggtgttcaacgtcgactcggccaccggctggggcctgaccaacgagagcaagcgggtcctcggcgacagcgcgcgcttcctcaacggcgactgccaccatccgcacatctcgatgaccgacggcaagtacgacggcaagtacctgttcatcaacgacaaggccaacagccgggtcgcgcgcatccgcctggacgtcatgaaatgcgaccgcatcgtcaccattcccaacgtccaggcgatccacggcctgcgcctgcaaaaggtgccgcatacccgctacgtgttctgcaacgccgagttcatcatcccccatcccaacgacggctcgaccttcgacctgtccggcgacaacgccttcaccctgtacaacgccatcgacgccgagaccatggaagtggcctggcaggtgatcgtcgacggcaacctcgacaacaccgacatggactacagcggcaggttcgccgcctccacctgctacaactcggagaaggccgtcgacctcggcggcatgatgcgcaacgagcgcgactgggtggtggtgttcgacatcccgcgcatcgaggccgagatcaaggcgaagcgcttcgtcaccctcggcgactcgaaggtgccggtggtcgacggccggcgcaaggacggcaaggacagcccggtgacccgctacatcccggtaccgaagaacccccacgggctgaacacctcgccggacggcaagtacttcatcgccaacggcaagctctcgccgacctgcaccatgatcgccatcgagcgcctcggcgacctgttcgccggcaagctggccgacccgcgcgacgtggtggtgggcgagccggaactgggcctcggcccgctgcacaccactttcgatggccgaggcaacgcctataccacgctgttcatcgacagccagttggtgaagtggaacctggccgacgcggtgcgcgcctacaagggcgagaaggtcgactacatccgccagaaactcgacgtgcagtaccagccggggcacaaccacgccaccctgtgcgagaccagcgaagccgacggcaagtggatcgtggtgctcagcaagttctccaaggaccgcttcctgcccaccggtccgctgcacccggagaacgaccagttgatcgacatttccggcgaggaaatgaagctggtccacgacggcccgaccttcgccgaaccgcacgattgcatcctcgcccgccgcgaccagatcaagacccgcaagatctgggaccgcaaggacccgttcttcgccgagacggtcaagcgcgcggaaaaggacggcatcgacctgatgaaggacaacaaggtcatccgcgagggcaacaaggtccgcgtctacatggtctcgatggcgccctccttcggcctcaccgagttcaaggtgaagcagggcgacgaagtcaccgtgaccatcaccaacctcgacgagatcgaggacgtgacccacggcttcgtcatggtcaaccacggcgtctgcatggagatcagcccgcaacagacctcgtcgatcaccttcgtcgccgataagcccggggtgcactggtactactgcagctggttctgccacgccctgcacatggaaatgtgcgggcggatgctggtggaaaaggcttga BBa_K864101_sequence 1 tcacacaggaaagtactagatggttagcaagggcgaagaactttttacaggcgtagtaccgatcttagttgaattagacggcgacgttaacggtcataagtttagcgtgagcggtgagggtgaaggtgacgcaacttacggcaagctgaccctgaagctgatttgcacgacgggtaagctgccggtcccgtggcctaccctggtcacgaccttgggttatggcgttcagtgtttcgcgcgttatccggaccacatgaaacaacacgatttctttaagagcgcgatgccagaaggctatgtgcaggagcgtacgatctttttcaaagacgacggtaactacaagacgcgtgccgaagtcaaattcgaaggcgacaccctggtgaatcgcattgagctgaagggtattgatttcaaagaggatggcaatatcctgggtcacaagctggagtacaattacaattcccacaacgtttacatcaccgcagataaacagaaaaatggcatcaaagcgaatttcaaaatccgtcacaacattgaggacggtggtgttcaactggcggatcattaccagcaaaacaccccgattggtgacggtccggtcctgttgccggataaccattatctgtcttaccaaagcaaactgagcaaagatccgaacgagaagcgcgaccacatggtgctgctggagtttgtgaccgctgccggtattaccctgggtatggatgagctgtataaataataa BBa_B0032_sequence 1 tcacacaggaaag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z