BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K1866009
1
BBa_K1866009
promoter+RibosomeBindingSite+Sqr (in pSB1C3)
2015-09-17T11:00:00Z
2015-09-18T05:15:09Z
Cyanococcus spp.
This part consists of the Sulfide Quinone Reductase Sqr gene under the control of a strong promoter with a Ribosome Binding Site. The Sqr gene catalyses the conversion of hydrogen Sulfide gas to elemental Sulfur.
false
false
_2296_
28697
28697
9
false
None. It is 3A compatible.
false
Stefan Oehler
component2474306
1
BBa_K880005
component2474310
1
BBa_K896000
annotation2474310
1
BBa_K896000
range2474310
1
62
1342
annotation2474306
1
BBa_K880005
range2474306
1
1
55
BBa_K880005
1
BBa_K880005
Strong promoter, strong RBS combination for high expression levels of proteins
2012-09-28T11:00:00Z
2015-05-08T01:13:40Z
It is a combination of strong promoter (J23100) and RBS (B0034).
Released HQ 2013
-J23100+B0034
-Strong promoter, strong RBS combination for high expression levels of proteins
Consensus constitutive promoter and RBS sequence-produces strongest possible expression.
false
false
_1142_
0
9403
9
In stock
false
Enter design considerations.
false
Josh Atkinson, Mike Ferguson, and Ben Parker
component2204230
1
BBa_B0034
component2204228
1
BBa_J23100
annotation2204230
1
BBa_B0034
range2204230
1
44
55
annotation2204228
1
BBa_J23100
range2204228
1
1
35
BBa_K896000
1
BBa_K896000
SQR(sulfide quinone reductase),from Synechococcus sp. PCC 7002 plasmid pAQ7
2012-09-08T11:00:00Z
2015-05-08T01:13:41Z
Synechococcus sp. PCC 7002 (strain: PCC 7002; ATCC 27264)
Location : plasmid: pAQ7
Sequence : NC_010474.1 (85060..86340)
AAAAABBBBBCCCCCC
false
true
_1161_
0
12739
9
In stock
true
DDDDDDEEEEEFFFFFF
false
Hsun-I Chiu
annotation2182658
1
K896000
range2182658
1
1
1281
annotation2182604
1
start
range2182604
1
1
3
annotation2182605
1
stop
range2182605
1
1279
1281
BBa_J23100
1
BBa_J23100
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
Isolated from library of promoters
Released HQ 2013
Replace later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_J23100_sequence
1
ttgacggctagctcagtcctaggtacagtgctagc
BBa_K1866009_sequence
1
ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatggctcatattgttgtaatcggtgctggtattggtggtctgccgacggcctatgaactgaggcacctgttgactaccgaccacactgttaccctcattgctgatacaccccactttacgtttatcccttctttgccctgggtggcccttggtctgaaaagattggatcaggtgcaattacccttgccaacgttggcccgtcgccatggtctgcattgggtacagggggctgtgcagcagattaatccccaggaaaggcatgttttggttgggtcagacgcaaaacgaattgactatgactatgtggtgatcgcaccgggcgcagctttaaatctcgatgctttaccgggtctcggtccagagacaggctttacccagtcagtttgtaatccccaccatgcactgcttgcccatgaagcttgggaaaaatttatccagaatccaggcccgttggtcgtgggggcggcaccgggggcgagttgttttggcccggcctatgagtttgcgttgttggcagattggcaactgcgacggttgggtttgcgggagcaggtaccgatcacattggtcaccccagaaccctatttgggtcatttggggattgggggcatggcccactcgcaggaattggttgagcaggttctccagcaacaggatattgcgacccgtgccaatgctgagattaccgccattaaaccggatatgattggtttggcggatggggaacagctaccctttgcctacagtatggttttgccgtcgttccaggggcctgcttttttgcgtgactgtccggcgatctccaattcccaggggtttattcccgtcttgcccacctatcaacatccagcgttcgattccgtgtatgcggcgggggtgattgttgaactaacgccccatgaagccacaccaatccccacgggtctaccgaaaacaggacaaatgacggaggcgatggggatggccgccgcccacaatattgcccgacagcttaactccaatctgggtgctccggtcacggcgaccctcgccgcaatttgtatgagtgattttggcgatcgcggcattatcttcatcgccgatccggtacagcgggagccaggcatggtcaaacgtcgccgctgtgtcgccctcgaaggacggtgggtgagttggagcaaaaccctttttgaactgtttttcctgacgaaaatgcgttggggtctaacgataccttggtttgagaaactgggcttaaagacattgggattgcagcttgtgcgtccccttcctccagactaa
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K896000_sequence
1
atggctcatattgttgtaatcggtgctggtattggtggtctgccgacggcctatgaactgaggcacctgttgactaccgaccacactgttaccctcattgctgatacaccccactttacgtttatcccttctttgccctgggtggcccttggtctgaaaagattggatcaggtgcaattacccttgccaacgttggcccgtcgccatggtctgcattgggtacagggggctgtgcagcagattaatccccaggaaaggcatgttttggttgggtcagacgcaaaacgaattgactatgactatgtggtgatcgcaccgggcgcagctttaaatctcgatgctttaccgggtctcggtccagagacaggctttacccagtcagtttgtaatccccaccatgcactgcttgcccatgaagcttgggaaaaatttatccagaatccaggcccgttggtcgtgggggcggcaccgggggcgagttgttttggcccggcctatgagtttgcgttgttggcagattggcaactgcgacggttgggtttgcgggagcaggtaccgatcacattggtcaccccagaaccctatttgggtcatttggggattgggggcatggcccactcgcaggaattggttgagcaggttctccagcaacaggatattgcgacccgtgccaatgctgagattaccgccattaaaccggatatgattggtttggcggatggggaacagctaccctttgcctacagtatggttttgccgtcgttccaggggcctgcttttttgcgtgactgtccggcgatctccaattcccaggggtttattcccgtcttgcccacctatcaacatccagcgttcgattccgtgtatgcggcgggggtgattgttgaactaacgccccatgaagccacaccaatccccacgggtctaccgaaaacaggacaaatgacggaggcgatggggatggccgccgcccacaatattgcccgacagcttaactccaatctgggtgctccggtcacggcgaccctcgccgcaatttgtatgagtgattttggcgatcgcggcattatcttcatcgccgatccggtacagcgggagccaggcatggtcaaacgtcgccgctgtgtcgccctcgaaggacggtgggtgagttggagcaaaaccctttttgaactgtttttcctgacgaaaatgcgttggggtctaacgataccttggtttgagaaactgggcttaaagacattgggattgcagcttgtgcgtccccttcctccagactaa
BBa_K880005_sequence
1
ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z