BBa_K1869001 1 BBa_K1869001 SAM Synthetase 2015-09-17T11:00:00Z 2015-09-18T12:49:32Z The SAM synthetase sequence was extracted, through PCR, from E. coli K-12 TG1. This protein is inherent in various organisms as it plays a major role in methionine and cysteine biosynthesis. The S-adenosylmethionine (SAM) Synthetase is a cytosolic protein the is responsible for catalyzing the reaction between methionine and ATP to produce SAM. SAM functions as the primary methyl donor in numerous methylation reactions. It also plays a major role in in the SAM cycle and cysteine biosynthesis. This protein can be used in conjunction with other proteins to increase the concentration of methyl donors in a culture to help drive forward reactions. When used in conjunction with BBa_K1869000, the CFA synthase, SAM drives the CFA synthesis reaction forward. false false _2301_ 26873 26873 9 false This sequence was extracted through PCR with over hangs designed to ligate into the pSB1C3 vector using the PIPE cloning method. false Conary Meyer annotation2469610 1 SAM Synthetase range2469610 1 1 1158 BBa_K1869001_sequence 1 atggcaaaacacctttttacgtccgagtccgtctctgaagggcatcctgacaaaattgctgaccaaatttctgatgccgttttagacgcgatcctcgaacaggatccgaaagcacgcgttgcttgcgaaacctacgtaaaaaccggcatggttttagttggcggcgaaatcaccaccagcgcctgggtagacatcgaagagatcacccgtaacaccgttcgcgaaattggctatgtgcattccgacatgggctttgacgctaactcctgtgcggttctgagcgctatcggcaaacagtctcctgacatcaaccagggcgttgaccgtgccgatccgctggaacagggcgcgggtgaccagggtctgatgtttggctacgcaactaatgaaaccgacgtgctgatgccagcacctatcacctatgcacaccgtctggtacagcgtcaggctgaagtgcgtaaaaacggcactctgccgtggctgcgcccggacgcgaaaagccaggtgacttttcagtatgacgacggcaaaatcgttggtatcgatgctgtcgtgctttccactcagcactctgaagagatcgaccagaaatcgctgcaagaagcggtaatggaagagatcatcaagccaattctgcccgctgaatggctgacttctgccaccaaattcttcatcaacccgaccggtcgtttcgttatcggtggcccaatgggtgactgcggtctgactggtcgtaaaattatcgttgatacctacggcggcatggcgcgtcacggtggcggtgcattctctggtaaagatccatcaaaagtggaccgttccgcagcctacgcagcacgttatgtcgcgaaaaacatcgttgctgctggcctggccgatcgttgtgaaattcaggtttcctacgcaatcggcgtggctgaaccgacctccatcatggtagaaactttcggtactgagaaagtgccttctgaacaactgaccctgctggtacgtgagttcttcgacctgcgcccatacggtctgattcagatgctggatctgctgcacccgatctacaaagaaaccgcagcatacggtcactttggtcgtgaacatttcccgtgggaaaaaaccgacaaagcgcagctgctgcgcgatgctgccggtctgaagtaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z