BBa_K187077
1
BBa_K187077
terminator reverse strand, Biobytes assembly method
2009-10-20T11:00:00Z
2015-05-08T01:11:08Z
Oligonucleotide synthesis
This part can be annealed to the terminator forward strand A (BBa_K187067) or the terminator forward strand B (BBa_K18790), to produce an A or B overhang respectively. The A overhang can anneal to the A' end on the 3' end of a part produced from pBA. The B overhang can anneal to the B' overhang on the end of a part produced from pAB.
The biobytes assembly method can be used to rapidly assemble DNA segments in a particular order. The 3' end of constructs made with this method can be annealed to a terminator segment, which can be digested and annealed to the anchor to circularize the construct.
For more details see http://2009.igem.org/Team:Alberta/DNAanchor, or RFC 47
false
false
_286_
0
4048
9
Not in stock
true
Once the construct has been completed, i.e. the last Byte has been added, the construct may be released from the beads as is by a simple I-SceI digestion or a USERTM digestion, thus yielding a linear construct. If a circular construct, such as a plasmid, is desired then a final "Terminator" piece must be added. This piece is similar in construction to the Anchor, whereby there is a dsDNA I-SceI recognition sequence with four deoxyuracils incorporated into it on one strand, as well as an A or B 3' overhang. The Terminator binds to the last Byte and release is once again achieved by I-SceI digestion or USERTM digestion. In either case both the Anchor and Terminator develop sticky ends that are complementary to eachother: 4 bases if I-SceI digestion is utilized, or 21 bases if USER is used. USERTM digestion is obviously preferred since 21 bp of interaction will form spontaneously and without ligation, and thus transformation of the construct can proceed immediately.
false
Julia Pon
annotation2061266
1
should have a phosphate
range2061266
1
1
1
BBa_K187077_sequence
1
atattaacctgttatccctat
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z