LOCUS       BBa_K1871001            1856 bp    DNA     linear       22-Nov-2024
DEFINITION  Alpha amylase with native secretion
ACCESSION   BBa_K1871001
VERSION     BBa_K1871001.1  
FEATURES             Location/Qualifiers
     promoter        1..104
                     /label=Minimal cyc Promoter
     RBS             105..125
                     /label=Kozak Sequence
     misc_feature    126..215
                     /label=Native Alpha Amylase Secretion Sequence
     CDS             216..1667
                     /label=Alpha Amylase Coding Sequence
     misc_feature    1668..1855
                     /label=ADH1 Terminator
ORIGIN
        1 ggcatgtgct ctgtatgtat ataaaactct tgttttcttc ttttctctaa atattctttc
       61 cttatacatt aggacctttg cagcataaat tactatactt ctatggatcc acgattaaaa
      121 gaatgatcca aaaacgaaag cggacagttt cgttcagatt agtgcttatg tgcacgctgt
      181 tatttgtcag tttgccgatt acaaaaacat cagccgtaaa tggcacgttg atgcagtact
      241 ttgaatggta tacgccgaac gacggacagc attggaaacg attgcagaat gatgcggaac
      301 atttgtcgga tatcggaatc accgccgtct ggattcctcc cgcatacaaa ggactgagcc
      361 aatccgataa cggatacggt ccatatgatt tgtatgattt aggagagttc cagcaaaaag
      421 ggacggtcag aacgaaatac ggcacaaaat cagagcttca agatgcgatc ggctcactgc
      481 attcccggaa cgtccaagta tacggagatg tggttttgaa ccataaggct ggtgctgatg
      541 caacagaaga tgtaactgcc gtcgaagtca atccggccaa tagaaatcag gaaacttcgg
      601 aggaatatca aatcaaagcg tggacggatt ttcgctttcc gggccgtgga aacacgtaca
      661 gtgattttaa atggcattgg tatcatttcg acggagcgga ctgggatgaa tcccggaaga
      721 tcagccgcat ctttaagttt cgtggggaag gaaaagcgtg ggattgggaa gtatcaagtg
      781 aaaacggcaa ctatgactat ttaatgtatg ctgatgttga ctacgaccac cctgatgtcg
      841 tggcagagac aaaaaaatgg ggtatctggt atgcgaatga actgtcatta gacggcttcc
      901 gtattgatgc cgccaaacat attaaatttt catttctgcg tgattgggtt caggcggtca
      961 gacaggcgac gggaaaagaa atgtttacgg ttgcggagta ttggcagaat aatgccggga
     1021 aactcgaaaa ctacttgaat aaaacaagct ttaatcaatc cgtgtttgat gttccgcttc
     1081 atttcaattt acaggcggct tcctcacaag gaggcggata tgatatgagg cgtttgctgg
     1141 acggtaccgt tgtgtccagg catccggaaa aggcggttac atttgttgaa aatcatgaca
     1201 cacagccggg acagtcattg gaatcgacag tccaaacttg gtttaaaccg cttgcatacg
     1261 cctttatttt gacaagagaa tccggttatc ctcaggtgtt ctatggggat atgtacggga
     1321 caaaagggac atcgccaaag gaaattccct cactgaaaga taatatagag ccgattttaa
     1381 aagcgcgtaa ggagtacgca tacgggcccc agcacgatta tattgaccac ccggatgtga
     1441 tcggatggac gagggaaggt gacagctccg ccgccaaatc aggtttggcc gctttaatca
     1501 cggacggacc cggcggatca aagcggatgt atgccggcct gaaaaatgcc ggcgagacat
     1561 ggtatgacat aacgggcaac cgttcagata ctgtaaaaat cggatctgac ggctggggag
     1621 agtttcatgt aaacgatggg tccgtctcca tttatgttca gaaataagcg aatttcttat
     1681 gatttatgat ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa
     1741 gtgactctta ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca
     1801 ggttgctttc tcaggtatag tatgaggtcg ctcttattga ccacacctct accggt
//