LOCUS BBa_K1871004 1856 bp DNA linear 24-Dec-2024 DEFINITION Skdk ACCESSION BBa_K1871004 VERSION BBa_K1871004.1 FEATURES Location/Qualifiers promoter 1..104 /label=Minimal cyc Promoter RBS 105..125 /label=Kozak Sequence misc_feature 126..215 /label=Native Alpha Amylase Secretion Sequence CDS 216..1667 /label=Alpha Amylase Coding Sequence misc_feature 1668..1855 /label=ADH1 Terminator misc_feature 1..1856 /label=BBa_K1871001 ORIGIN 1 ggcatgtgct ctgtatgtat ataaaactct tgttttcttc ttttctctaa atattctttc 61 cttatacatt aggacctttg cagcataaat tactatactt ctatggatcc acgattaaaa 121 gaatgatcca aaaacgaaag cggacagttt cgttcagatt agtgcttatg tgcacgctgt 181 tatttgtcag tttgccgatt acaaaaacat cagccgtaaa tggcacgttg atgcagtact 241 ttgaatggta tacgccgaac gacggacagc attggaaacg attgcagaat gatgcggaac 301 atttgtcgga tatcggaatc accgccgtct ggattcctcc cgcatacaaa ggactgagcc 361 aatccgataa cggatacggt ccatatgatt tgtatgattt aggagagttc cagcaaaaag 421 ggacggtcag aacgaaatac ggcacaaaat cagagcttca agatgcgatc ggctcactgc 481 attcccggaa cgtccaagta tacggagatg tggttttgaa ccataaggct ggtgctgatg 541 caacagaaga tgtaactgcc gtcgaagtca atccggccaa tagaaatcag gaaacttcgg 601 aggaatatca aatcaaagcg tggacggatt ttcgctttcc gggccgtgga aacacgtaca 661 gtgattttaa atggcattgg tatcatttcg acggagcgga ctgggatgaa tcccggaaga 721 tcagccgcat ctttaagttt cgtggggaag gaaaagcgtg ggattgggaa gtatcaagtg 781 aaaacggcaa ctatgactat ttaatgtatg ctgatgttga ctacgaccac cctgatgtcg 841 tggcagagac aaaaaaatgg ggtatctggt atgcgaatga actgtcatta gacggcttcc 901 gtattgatgc cgccaaacat attaaatttt catttctgcg tgattgggtt caggcggtca 961 gacaggcgac gggaaaagaa atgtttacgg ttgcggagta ttggcagaat aatgccggga 1021 aactcgaaaa ctacttgaat aaaacaagct ttaatcaatc cgtgtttgat gttccgcttc 1081 atttcaattt acaggcggct tcctcacaag gaggcggata tgatatgagg cgtttgctgg 1141 acggtaccgt tgtgtccagg catccggaaa aggcggttac atttgttgaa aatcatgaca 1201 cacagccggg acagtcattg gaatcgacag tccaaacttg gtttaaaccg cttgcatacg 1261 cctttatttt gacaagagaa tccggttatc ctcaggtgtt ctatggggat atgtacggga 1321 caaaagggac atcgccaaag gaaattccct cactgaaaga taatatagag ccgattttaa 1381 aagcgcgtaa ggagtacgca tacgggcccc agcacgatta tattgaccac ccggatgtga 1441 tcggatggac gagggaaggt gacagctccg ccgccaaatc aggtttggcc gctttaatca 1501 cggacggacc cggcggatca aagcggatgt atgccggcct gaaaaatgcc ggcgagacat 1561 ggtatgacat aacgggcaac cgttcagata ctgtaaaaat cggatctgac ggctggggag 1621 agtttcatgt aaacgatggg tccgtctcca tttatgttca gaaataagcg aatttcttat 1681 gatttatgat ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa 1741 gtgactctta ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca 1801 ggttgctttc tcaggtatag tatgaggtcg ctcttattga ccacacctct accggt //