BBa_K187425
1
BBa_K187425
Origin in AB, Reverse primer
2009-10-20T11:00:00Z
2015-05-08T01:11:13Z
Synthetic
The pUC19 origin can be amplified from pAB or pBA using this primer in conjunction with the Origin AB forward primer. A high copy origin can be produced with A and B ends compatible with the BioBytes assembly method. Origins cannot be cloned into pAB and pBA using the standard BioBytes method because the presence of two origins will destabilize the plasmid. Some thymine bases in the above sequence must be replaced with uracil for correct function. Actual sequence is as follows:
5' AGCTGUAGTATUGCCGCGTTGCTGGCG 3'
This part will allow amplification of a high copy origin to allow proliferation of an artificial BioBytes plasmid. See BBF RFC#47 for information on the BioBytes method.
false
false
_286_
0
4104
9
Not in stock
true
Primer was designed to give standard BioBytes B end. Uracil separation was experimentally optimized.
false
Mitch Paquette
annotation2061302
1
should be uracil
range2061302
1
6
6
annotation2061303
1
should be uracil
range2061303
1
12
12
BBa_K187425_sequence
1
agctgtagtattgccgcgttgctggcg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z