BBa_K1875012
1
BBa_K1875012
This part produces a guide RNA that pairs with an operator.
2016-10-10T11:00:00Z
2016-10-15T11:43:59Z
This plasmid was based off of the work by Feng Zhanf at MIT and the x330 plasmid
This composite part can be used to express a guide RNA (g8) from BostonU 2016???s project Gemini. Specifically, this part expresses g8, a guide RNA that directly recognizes the 20bp target sequence 5??? GTTGCGCGTCCGTATCAAGG 3???. This 20bp target sequence can be found in composite part BBa_K1875004.
We used transient transfection into HEK293FT cells to validate functionality of this part. We co-delivered a plasmid with BBa_K1875001 (this composite part with g8), a plasmid with BBa_K1875004 (the corresponding GFP reporter composite part with g8???s target sequence), and a plasmid expressing dCas9-VPR. We assayed for fluorescence of GFP using flow cytometry. We compared expression levels of GFP both with and without the guide RNA. Our results indicate that there is a low level of expression without g8 and a high level of expression with g8.
false
false
_2340_
32298
32298
9
false
This part produces the guide in BBa_K1875006 and the guide that matches the target sequence on BBa_K1875015
false
Jeffrey Marano
BBa_K1875012_sequence
1
gagggcctatttcccatgattccttcatatttgcatatacgatacaaggctgttagagagataattggaattaatttgactgtaaacacaaagatattagtacaaaatacgtgacgtagaaagtaataatttcttgggtagtttgcagttttaaaattatgttttaaaatggactatcatatgcttaccgtaacttgaaagtatttcgatttcttggctttatatatcttgtggaaaggacgaaacaccggttgcgcgtccgtatcaagggttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgctttttt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z