BBa_K1875002 1 BBa_K1875002 This part contains an RBG polyA terminator 2016-10-10T11:00:00Z 2016-10-15T11:22:44Z The Rabbit Beta Globin Poly A terminator was taken from work done by Connie Cepko. This part contains a Rabbit Beta Globin Poly A terminator. This termination sequence is used in the following composite parts: BBa_K1875013, BBa_K1875014, BBa_K1875015, BBa_K1875016, BBa_K1875017, BBa_K1875018, BBa_K1875019. false false _2340_ 32298 32298 9 false This part was acquired and not designed by our lab. false Jeffrey Marano BBa_K1875001 1 BBa_K1875001 This part contains a Kozak sequence 2016-10-10T11:00:00Z 2016-10-15T11:22:08Z This part is a consensus sequence and thus has no source. The Kozak sequence that makes up this part is integral to the initiation of translation in eukaryotic cells. This part is used in the following composite parts: following parts: BBa_K1875013, BBa_K1875014, BBa_K1875015, BBa_K1875016, BBa_K1875017, BBa_K1875018, BBa_K1875019. false false _2340_ 32298 32298 9 false This part was designed and synthesized based on the known Kozak sequence. false Jeffrey Marano BBa_K1875003 1 BBa_K1875003 This part contains a GFP reporter 2016-10-10T11:00:00Z 2016-10-15T11:23:40Z This GFP was acquired from Connie Cepko's lab. This GFP reporter is used in all of the following plasmids: BBa_K1875013, BBa_K1875014, BBa_K1875015, BBa_K1875016, BBa_K1875017, BBa_K1875018, BBa_K1875019 false false _2340_ 32298 32298 9 false This part was not designed by our lab, but rather was acquired. false Jeffrey Marano BBa_K1875013 1 BBa_K1875013 This operator, when paired with a guide RNA, expresses GFP 2016-10-10T11:00:00Z 2016-10-11T05:49:50Z Please see the Subparts pages to find sources of this part. Single Operator The BostonU 2016 iGEM team set out to design a set of mutually orthogonal guide RNA (gRNA) expressing and guide RNA operator pairs that could activate the expression of a gene of interest. This part contains target sequence for g1 from BostonU???s Gemini Library (TCTGCCCGAAGTTTCGACGG). As figure 1 indicates, the introduction of the gRNA leads to a several fold increase in expression in comparison to the basal level without the gRNA. The matrix, figure 2, illustrates the results from a mutual orthogonality screen showing on target and off target expression levels among the guide expression vectors and the guide operator vectors in our system . The results from this experiment show that there is no significant expression among off target pairs, demonstrating that there is no cross talk in Gemini. false false _2340_ 0 32298 32298 9 It's complicated false Insert Later false Jeffrey Marano component2493055 1 BBa_K1875003 component2493053 1 BBa_K1875000 component2493054 1 BBa_K1875001 component2493052 1 BBa_K1875005 component2493056 1 BBa_K1875002 annotation2493053 1 BBa_K1875000 range2493053 1 29 98 annotation2493055 1 BBa_K1875003 range2493055 1 122 841 annotation2493056 1 BBa_K1875002 range2493056 1 850 1385 annotation2493054 1 BBa_K1875001 range2493054 1 107 115 annotation2493052 1 BBa_K1875005 range2493052 1 1 20 BBa_K1875000 1 BBa_K1875000 This basic part contains a miniCMV promoter. 2016-10-10T11:00:00Z 2016-10-15T11:21:18Z This promoter was obtained from George Church and the Church Lab. The miniCMV promoter is the driving promoter for the following parts: BBa_K1875013, BBa_K1875014, BBa_K1875015, BBa_K1875016, BBa_K1875017, BBa_K1875018, BBa_K1875019. false false _2340_ 32298 32298 9 false Our lab did not design this sequence. false Jeffrey Marano BBa_K1875005 1 BBa_K1875005 A 20 bp target sequence and PAM to make guide operator 1 2016-10-10T11:00:00Z 2016-10-18T11:14:43Z This sequence was synthesized by IDT This part contains the guide that serves as a target sequence on the BBa_K1875013 false false _2340_ 32290 32298 9 false This sequence was designed based on its orthogonality to the human genome (hg19). false Jeffrey Marano BBa_K1875003_sequence 1 atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaa BBa_K1875000_sequence 1 cgaggtaggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaaccgtcagatcgcctgga BBa_K1875001_sequence 1 gccgccacc BBa_K1875005_sequence 1 tctgcccgaagtttcgacggggg BBa_K1875013_sequence 1 tctgcccgaagtttcgacggtactagagcgaggtaggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaaccgtcagatcgcctggatactagaggccgccacctactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaatactagagactcctcaggtgcaggctgcctatcagaaggtggtggctggtgtggccaatgccctggctcacaaataccactgagatctttttccctctgccaaaaattatggggacatcatgaagccccttgagcatctgacttctggctaataaaggaaatttattttcattgcaatagtgtgttggaattttttgtgtctctcactcggaaggacatatgggagggcaaatcatttaaaacatcagaatgagtatttggtttagagtttggcaacatatgcccatatgctggctgccatgaacaaaggttggctataaagaggtcatcagtatatgaaacagccccctgctgtccattccttattccatagaaaagccttgacttgaggttagattttttttatattttgttttgtgttatttttttctttaacatccctaaaattttccttacatgttttactagccagatttttcctcctctcctgactactcccagtcatagctgtccctcttctcttatggagatccctcgacctgca BBa_K1875002_sequence 1 actcctcaggtgcaggctgcctatcagaaggtggtggctggtgtggccaatgccctggctcacaaataccactgagatctttttccctctgccaaaaattatggggacatcatgaagccccttgagcatctgacttctggctaataaaggaaatttattttcattgcaatagtgtgttggaattttttgtgtctctcactcggaaggacatatgggagggcaaatcatttaaaacatcagaatgagtatttggtttagagtttggcaacatatgcccatatgctggctgccatgaacaaaggttggctataaagaggtcatcagtatatgaaacagccccctgctgtccattccttattccatagaaaagccttgacttgaggttagattttttttatattttgttttgtgttatttttttctttaacatccctaaaattttccttacatgttttactagccagatttttcctcctctcctgactactcccagtcatagctgtccctcttctcttatggagatccctcgacctgca igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z