BBa_K1875002 1 BBa_K1875002 This part contains an RBG polyA terminator 2016-10-10T11:00:00Z 2016-10-15T11:22:44Z The Rabbit Beta Globin Poly A terminator was taken from work done by Connie Cepko. This part contains a Rabbit Beta Globin Poly A terminator. This termination sequence is used in the following composite parts: BBa_K1875013, BBa_K1875014, BBa_K1875015, BBa_K1875016, BBa_K1875017, BBa_K1875018, BBa_K1875019. false false _2340_ 32298 32298 9 false This part was acquired and not designed by our lab. false Jeffrey Marano BBa_K1875015 1 BBa_K1875015 This operator, when paired with a guide RNA, expresses GFP. 2016-10-10T11:00:00Z 2016-10-11T06:04:31Z See basic parts for the sources of this plasmid Single Operator The BostonU 2016 iGEM team set out to design a set of mutually orthogonal guide RNA (gRNA) expressing and guide RNA operator pairs that could activate the expression of a gene of interest. This part contains target sequence for g8 from BostonU???s Gemini Library (GTTGCGCGTCCGTATCAAGG). As figure 1 indicates, the introduction of the gRNA leads to a several fold increase in expression in comparison to the basal level without the gRNA. The matrix, figure 2, illustrates the results from a mutual orthogonality screen showing on target and off target expression levels among the guide expression vectors and the guide operator vectors in our system . The results from this experiment show that there is no significant expression among off target pairs, demonstrating that there is no cross talk in Gemini. false false _2340_ 0 32298 32298 9 It's complicated false Insert false Jeffrey Marano component2493081 1 BBa_K1875002 component2493077 1 BBa_K1875006 component2493079 1 BBa_K1875001 component2493080 1 BBa_K1875003 component2493078 1 BBa_K1875000 annotation2493081 1 BBa_K1875002 range2493081 1 853 1388 annotation2493078 1 BBa_K1875000 range2493078 1 32 101 annotation2493080 1 BBa_K1875003 range2493080 1 125 844 annotation2493077 1 BBa_K1875006 range2493077 1 1 23 annotation2493079 1 BBa_K1875001 range2493079 1 110 118 BBa_K1875001 1 BBa_K1875001 This part contains a Kozak sequence 2016-10-10T11:00:00Z 2016-10-15T11:22:08Z This part is a consensus sequence and thus has no source. The Kozak sequence that makes up this part is integral to the initiation of translation in eukaryotic cells. This part is used in the following composite parts: following parts: BBa_K1875013, BBa_K1875014, BBa_K1875015, BBa_K1875016, BBa_K1875017, BBa_K1875018, BBa_K1875019. false false _2340_ 32298 32298 9 false This part was designed and synthesized based on the known Kozak sequence. false Jeffrey Marano BBa_K1875006 1 BBa_K1875006 A 20 bp target sequence and PAM to make guide operator 8 2016-10-10T11:00:00Z 2016-10-18T11:15:46Z This sequence was synthesized by IDT This guide is expressed by BBa_K1875012 and is the target sequence on BBa_K1875015 false false _2340_ 32290 32298 9 false This sequence was designed based on its orthogonality to the human genome (hg19). false Jeffrey Marano BBa_K1875003 1 BBa_K1875003 This part contains a GFP reporter 2016-10-10T11:00:00Z 2016-10-15T11:23:40Z This GFP was acquired from Connie Cepko's lab. This GFP reporter is used in all of the following plasmids: BBa_K1875013, BBa_K1875014, BBa_K1875015, BBa_K1875016, BBa_K1875017, BBa_K1875018, BBa_K1875019 false false _2340_ 32298 32298 9 false This part was not designed by our lab, but rather was acquired. false Jeffrey Marano BBa_K1875000 1 BBa_K1875000 This basic part contains a miniCMV promoter. 2016-10-10T11:00:00Z 2016-10-15T11:21:18Z This promoter was obtained from George Church and the Church Lab. The miniCMV promoter is the driving promoter for the following parts: BBa_K1875013, BBa_K1875014, BBa_K1875015, BBa_K1875016, BBa_K1875017, BBa_K1875018, BBa_K1875019. false false _2340_ 32298 32298 9 false Our lab did not design this sequence. false Jeffrey Marano BBa_K1875003_sequence 1 atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaa BBa_K1875015_sequence 1 gttgcgcgtccgtatcaagggggtactagagcgaggtaggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaaccgtcagatcgcctggatactagaggccgccacctactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaatactagagactcctcaggtgcaggctgcctatcagaaggtggtggctggtgtggccaatgccctggctcacaaataccactgagatctttttccctctgccaaaaattatggggacatcatgaagccccttgagcatctgacttctggctaataaaggaaatttattttcattgcaatagtgtgttggaattttttgtgtctctcactcggaaggacatatgggagggcaaatcatttaaaacatcagaatgagtatttggtttagagtttggcaacatatgcccatatgctggctgccatgaacaaaggttggctataaagaggtcatcagtatatgaaacagccccctgctgtccattccttattccatagaaaagccttgacttgaggttagattttttttatattttgttttgtgttatttttttctttaacatccctaaaattttccttacatgttttactagccagatttttcctcctctcctgactactcccagtcatagctgtccctcttctcttatggagatccctcgacctgca BBa_K1875000_sequence 1 cgaggtaggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaaccgtcagatcgcctgga BBa_K1875001_sequence 1 gccgccacc BBa_K1875006_sequence 1 gttgcgcgtccgtatcaaggggg BBa_K1875002_sequence 1 actcctcaggtgcaggctgcctatcagaaggtggtggctggtgtggccaatgccctggctcacaaataccactgagatctttttccctctgccaaaaattatggggacatcatgaagccccttgagcatctgacttctggctaataaaggaaatttattttcattgcaatagtgtgttggaattttttgtgtctctcactcggaaggacatatgggagggcaaatcatttaaaacatcagaatgagtatttggtttagagtttggcaacatatgcccatatgctggctgccatgaacaaaggttggctataaagaggtcatcagtatatgaaacagccccctgctgtccattccttattccatagaaaagccttgacttgaggttagattttttttatattttgttttgtgttatttttttctttaacatccctaaaattttccttacatgttttactagccagatttttcctcctctcctgactactcccagtcatagctgtccctcttctcttatggagatccctcgacctgca igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z