BBa_K1875002 1 BBa_K1875002 This part contains an RBG polyA terminator 2016-10-10T11:00:00Z 2016-10-15T11:22:44Z The Rabbit Beta Globin Poly A terminator was taken from work done by Connie Cepko. This part contains a Rabbit Beta Globin Poly A terminator. This termination sequence is used in the following composite parts: BBa_K1875013, BBa_K1875014, BBa_K1875015, BBa_K1875016, BBa_K1875017, BBa_K1875018, BBa_K1875019. false false _2340_ 32298 32298 9 false This part was acquired and not designed by our lab. false Jeffrey Marano BBa_K1875001 1 BBa_K1875001 This part contains a Kozak sequence 2016-10-10T11:00:00Z 2016-10-15T11:22:08Z This part is a consensus sequence and thus has no source. The Kozak sequence that makes up this part is integral to the initiation of translation in eukaryotic cells. This part is used in the following composite parts: following parts: BBa_K1875013, BBa_K1875014, BBa_K1875015, BBa_K1875016, BBa_K1875017, BBa_K1875018, BBa_K1875019. false false _2340_ 32298 32298 9 false This part was designed and synthesized based on the known Kozak sequence. false Jeffrey Marano BBa_K1875003 1 BBa_K1875003 This part contains a GFP reporter 2016-10-10T11:00:00Z 2016-10-15T11:23:40Z This GFP was acquired from Connie Cepko's lab. This GFP reporter is used in all of the following plasmids: BBa_K1875013, BBa_K1875014, BBa_K1875015, BBa_K1875016, BBa_K1875017, BBa_K1875018, BBa_K1875019 false false _2340_ 32298 32298 9 false This part was not designed by our lab, but rather was acquired. false Jeffrey Marano BBa_K1875000 1 BBa_K1875000 This basic part contains a miniCMV promoter. 2016-10-10T11:00:00Z 2016-10-15T11:21:18Z This promoter was obtained from George Church and the Church Lab. The miniCMV promoter is the driving promoter for the following parts: BBa_K1875013, BBa_K1875014, BBa_K1875015, BBa_K1875016, BBa_K1875017, BBa_K1875018, BBa_K1875019. false false _2340_ 32298 32298 9 false Our lab did not design this sequence. false Jeffrey Marano BBa_K1875018 1 BBa_K1875018 This operator, when paired with a guide RNA, expresses GFP. 2016-10-10T11:00:00Z 2016-10-11T06:24:32Z Please see basic parts pages for sources in the plasmid. Triple Operator The BostonU 2016 iGEM team???s Gemini Library contains analog parts in addition to their digital parts. They synthesized multimerized binding sites to increase the expression of the operator. This hypothesis was supported by analogous results in previous synthetic transcriptional regulator research. A multimerized operator means that there are consecutive operators upstream of the promoter where the dCas9-VPR and guide RNA will bind to. In addition to multimerizing operator sites, BostonU 2016 also modulated the space between the binding sites. The graph below demonstrates a screen with this multimerized operator containing the target for g13 from the Gemini Library (guide sequence: GTTCTAAACGTTGGTCCGTC). BostonU 2016 chose to submit the strongest of its triple multimerized operators which had a 24 base pair spacer between the operators. false false _2340_ 32298 32298 9 false Insert Later false Jeffrey Marano component2493097 1 BBa_K1875009 component2493101 1 BBa_K1875002 component2493099 1 BBa_K1875001 component2493100 1 BBa_K1875003 component2493098 1 BBa_K1875000 annotation2493098 1 BBa_K1875000 range2493098 1 126 195 annotation2493100 1 BBa_K1875003 range2493100 1 219 938 annotation2493099 1 BBa_K1875001 range2493099 1 204 212 annotation2493097 1 BBa_K1875009 range2493097 1 1 117 annotation2493101 1 BBa_K1875002 range2493101 1 947 1482 BBa_K1875009 1 BBa_K1875009 Three repeated guide operators to make triple operator 13 2016-10-10T11:00:00Z 2016-10-19T10:21:15Z This part was synthesized by IDT This part contains the target sequence for BBa_K1875018 false false _2340_ 32290 32298 9 false This part was designed by placing a random 24 base spacer between three g13 guides that make up BBa_K1875007 false Jeffrey Marano BBa_K1875003_sequence 1 atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaa BBa_K1875018_sequence 1 gttctaaacgttggtccgtcgggtctattcgaactgcgcgaaagttcgttctaaacgttggtccgtcgggtctattcgaactgcgcgaaagttcgttctaaacgttggtccgtcgggtactagagcgaggtaggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaaccgtcagatcgcctggatactagaggccgccacctactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaatactagagactcctcaggtgcaggctgcctatcagaaggtggtggctggtgtggccaatgccctggctcacaaataccactgagatctttttccctctgccaaaaattatggggacatcatgaagccccttgagcatctgacttctggctaataaaggaaatttattttcattgcaatagtgtgttggaattttttgtgtctctcactcggaaggacatatgggagggcaaatcatttaaaacatcagaatgagtatttggtttagagtttggcaacatatgcccatatgctggctgccatgaacaaaggttggctataaagaggtcatcagtatatgaaacagccccctgctgtccattccttattccatagaaaagccttgacttgaggttagattttttttatattttgttttgtgttatttttttctttaacatccctaaaattttccttacatgttttactagccagatttttcctcctctcctgactactcccagtcatagctgtccctcttctcttatggagatccctcgacctgca BBa_K1875009_sequence 1 gttctaaacgttggtccgtcgggtctattcgaactgcgcgaaagttcgttctaaacgttggtccgtcgggtctattcgaactgcgcgaaagttcgttctaaacgttggtccgtcggg BBa_K1875000_sequence 1 cgaggtaggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaaccgtcagatcgcctgga BBa_K1875001_sequence 1 gccgccacc BBa_K1875002_sequence 1 actcctcaggtgcaggctgcctatcagaaggtggtggctggtgtggccaatgccctggctcacaaataccactgagatctttttccctctgccaaaaattatggggacatcatgaagccccttgagcatctgacttctggctaataaaggaaatttattttcattgcaatagtgtgttggaattttttgtgtctctcactcggaaggacatatgggagggcaaatcatttaaaacatcagaatgagtatttggtttagagtttggcaacatatgcccatatgctggctgccatgaacaaaggttggctataaagaggtcatcagtatatgaaacagccccctgctgtccattccttattccatagaaaagccttgacttgaggttagattttttttatattttgttttgtgttatttttttctttaacatccctaaaattttccttacatgttttactagccagatttttcctcctctcctgactactcccagtcatagctgtccctcttctcttatggagatccctcgacctgca igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z