BBa_K1875002 1 BBa_K1875002 This part contains an RBG polyA terminator 2016-10-10T11:00:00Z 2016-10-15T11:22:44Z The Rabbit Beta Globin Poly A terminator was taken from work done by Connie Cepko. This part contains a Rabbit Beta Globin Poly A terminator. This termination sequence is used in the following composite parts: BBa_K1875013, BBa_K1875014, BBa_K1875015, BBa_K1875016, BBa_K1875017, BBa_K1875018, BBa_K1875019. false false _2340_ 32298 32298 9 false This part was acquired and not designed by our lab. false Jeffrey Marano BBa_K1875001 1 BBa_K1875001 This part contains a Kozak sequence 2016-10-10T11:00:00Z 2016-10-15T11:22:08Z This part is a consensus sequence and thus has no source. The Kozak sequence that makes up this part is integral to the initiation of translation in eukaryotic cells. This part is used in the following composite parts: following parts: BBa_K1875013, BBa_K1875014, BBa_K1875015, BBa_K1875016, BBa_K1875017, BBa_K1875018, BBa_K1875019. false false _2340_ 32298 32298 9 false This part was designed and synthesized based on the known Kozak sequence. false Jeffrey Marano BBa_K1875003 1 BBa_K1875003 This part contains a GFP reporter 2016-10-10T11:00:00Z 2016-10-15T11:23:40Z This GFP was acquired from Connie Cepko's lab. This GFP reporter is used in all of the following plasmids: BBa_K1875013, BBa_K1875014, BBa_K1875015, BBa_K1875016, BBa_K1875017, BBa_K1875018, BBa_K1875019 false false _2340_ 32298 32298 9 false This part was not designed by our lab, but rather was acquired. false Jeffrey Marano BBa_K1875019 1 BBa_K1875019 This operator, when paired with a guide RNA, expresses GFP. 2016-10-10T11:00:00Z 2016-10-11T06:27:50Z See basic parts for sources of this plasmid The BostonU 2016 iGEM team???s Gemini Library contains analog parts in addition to their digital parts. They synthesized mutated binding sites to decrease the expression of the operator. This hypothesis was supported by previous research suggesting that a mutated operator reduces the binding affinity of the dCas9-VPR complex. They used two mutation techniques: sequential mutation of a single base in the guide, beginning at the 5??? end, and clustered mutations at either base 1 or base 11. The altered base was determined by whether the base was a purine or a pyrimidine and the complement of the base. If the base was a purine, it was mutated to be a pyrimidine and of the opposite coupled nucleotide (i.e: A <-->C, G<-->T). The graph below demonstrates a screen with this mutated operator containing g13 from the Gemini Library (guide sequence: GTTCTAAACGTTGGTCCGTC). BostonU 2016 chose to submit the mutant operator with mutations at base 10 resulting in a sequence of GTTCTAAACTTTGGTCCGTC. The motivation behind this choice was the near five fold decrease in expression in comparison to the non-mutated operator. false false _2340_ 32298 32298 9 false Insert false Jeffrey Marano component2493104 1 BBa_K1875001 component2493103 1 BBa_K1875000 component2493102 1 BBa_K1875010 component2493105 1 BBa_K1875003 component2493106 1 BBa_K1875002 annotation2493104 1 BBa_K1875001 range2493104 1 110 118 annotation2493105 1 BBa_K1875003 range2493105 1 125 844 annotation2493102 1 BBa_K1875010 range2493102 1 1 23 annotation2493106 1 BBa_K1875002 range2493106 1 853 1388 annotation2493103 1 BBa_K1875000 range2493103 1 32 101 BBa_K1875010 1 BBa_K1875010 Base 10 target mutation to make mutated guide operator 13 2016-10-10T11:00:00Z 2016-10-19T11:05:16Z This part was synthesized by IDT This part contains the target sequence for BBa_K1875019 false false _2340_ 32290 32298 9 false The 10th base of BBa_K1875007 was mutated from a G to a T false Jeffrey Marano BBa_K1875000 1 BBa_K1875000 This basic part contains a miniCMV promoter. 2016-10-10T11:00:00Z 2016-10-15T11:21:18Z This promoter was obtained from George Church and the Church Lab. The miniCMV promoter is the driving promoter for the following parts: BBa_K1875013, BBa_K1875014, BBa_K1875015, BBa_K1875016, BBa_K1875017, BBa_K1875018, BBa_K1875019. false false _2340_ 32298 32298 9 false Our lab did not design this sequence. false Jeffrey Marano BBa_K1875003_sequence 1 atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaa BBa_K1875000_sequence 1 cgaggtaggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaaccgtcagatcgcctgga BBa_K1875010_sequence 1 gttctaaactttggtccgtcggg BBa_K1875001_sequence 1 gccgccacc BBa_K1875019_sequence 1 gttctaaactttggtccgtcgggtactagagcgaggtaggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaaccgtcagatcgcctggatactagaggccgccacctactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaatactagagactcctcaggtgcaggctgcctatcagaaggtggtggctggtgtggccaatgccctggctcacaaataccactgagatctttttccctctgccaaaaattatggggacatcatgaagccccttgagcatctgacttctggctaataaaggaaatttattttcattgcaatagtgtgttggaattttttgtgtctctcactcggaaggacatatgggagggcaaatcatttaaaacatcagaatgagtatttggtttagagtttggcaacatatgcccatatgctggctgccatgaacaaaggttggctataaagaggtcatcagtatatgaaacagccccctgctgtccattccttattccatagaaaagccttgacttgaggttagattttttttatattttgttttgtgttatttttttctttaacatccctaaaattttccttacatgttttactagccagatttttcctcctctcctgactactcccagtcatagctgtccctcttctcttatggagatccctcgacctgca BBa_K1875002_sequence 1 actcctcaggtgcaggctgcctatcagaaggtggtggctggtgtggccaatgccctggctcacaaataccactgagatctttttccctctgccaaaaattatggggacatcatgaagccccttgagcatctgacttctggctaataaaggaaatttattttcattgcaatagtgtgttggaattttttgtgtctctcactcggaaggacatatgggagggcaaatcatttaaaacatcagaatgagtatttggtttagagtttggcaacatatgcccatatgctggctgccatgaacaaaggttggctataaagaggtcatcagtatatgaaacagccccctgctgtccattccttattccatagaaaagccttgacttgaggttagattttttttatattttgttttgtgttatttttttctttaacatccctaaaattttccttacatgttttactagccagatttttcctcctctcctgactactcccagtcatagctgtccctcttctcttatggagatccctcgacctgca igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z