LOCUS BBa_K1877011 888 bp DNA linear 22-Nov-2024 DEFINITION Tetr Promoter+RBS+GFP+Terminator ACCESSION BBa_K1877011 VERSION BBa_K1877011.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 CDS 81..800 /label=GFP protein CDS 81..800 /label=BBa_E0040 stem_loop 820..863 /label=stem_loop misc_feature 809..888 /label=BBa_B0010 terminator 809..888 /label=BBa_B0010 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa 121 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg 181 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac 241 ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat 301 acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac 361 aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt 421 ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg 481 gaaacattct tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg 541 cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg 601 gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc 661 ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa 721 agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg 781 atgaactata caaataataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg 841 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctc //