BBa_K239006 1 BBa_K239006 Modified NarK promoter 2009-06-25T11:00:00Z 2015-05-08T01:11:36Z Raw genomic sequence from biocyc, Escherichia coli K-12, but modified. Sequence contains 2 Fnr (one of which is modified), 1 Fis, 2 NarL binding sites and initiates transcription by RNAP sigma-70. Function: Switched on during anaerobic conditions. Area of application: Detection of when the cell is switching to anaerobic metabolism. false false _375_ 0 4247 9 It's complicated false Detect and remove possible restriction sites: XbaI, EcoRI, PstI, SpeI, AgeI, BamHI, BglII, NogMIV, NotI and XhoI. No restriction site had to be removed. Sequence starts 3 bp before the first FNR binding site and ends 6 pb after the -10 box. One pb has been changed in order optimise the first FNR binding site. false Axel Nystrom annotation2006723 1 Fis range2006723 1 38 56 annotation2006722 1 Fnr range2006722 1 42 55 annotation2006725 1 NarL range2006725 1 20 26 annotation2006720 1 -10 range2006720 1 76 83 annotation2006726 1 NarL range2006726 1 7 13 annotation2006724 1 Fnr range2006724 1 4 17 annotation2006721 1 -35 range2006721 1 48 56 BBa_K1878007 1 BBa_K1878007 mNarK/iRFP713 2016-10-13T11:00:00Z 2016-10-14T09:09:40Z This gene sequence was procured by IDT. Hypoxia inducible iRFP713 with modified NarK promoter false false _2343_ 26215 26215 9 false Fickle false Margaret Lie component2511724 1 BBa_K239006 component2511725 1 BBa_K1878001 annotation2511724 1 BBa_K239006 range2511724 1 1 89 annotation2511725 1 BBa_K1878001 range2511725 1 96 1046 BBa_K1878001 1 BBa_K1878001 iRFP713 (the fluorescent protein formerly known as "iRFP") 2016-10-13T11:00:00Z 2016-10-26T01:41:54Z iRFP713 is a Rhodopseudomonas palustris bacterial phytochrome photoreceptor. This fluorescent protein has an excitation wavelength of 690 nm and an emission wavelength of 713 nm. The extinction coefficient is 98000 M-1 cm-1. Its quantum yield is 6.3%. Its photostability half-life is 960 seconds. Its pKa is 4.5. Near-infrared fluorescent proteins are good for long-term in vivo imaging and exhibit high brightness in bacteria, mammalian cells, and mice. false false _2343_ 25183 25183 9 false The PstI site and EcoRI site within the reporter has been removed. There is a Bbs1 site within the coding sequence so be wary if using that enzyme for Golden Gate DNA assembly. false John Luke Garcia BBa_K1878007_sequence 1 gtattgataaatatcaatgatagataaagttatcttatcgtttgatttacatcaaattgcctttagctacagacactaaggtggcagactactagatgacagaaggatccgtcgccaggcagcctgacctcttgacctgcgacgatgagccgatccatatccccggtgccatccaaccgcatggactgctgctcgccctcgccgccgacatgacgatcgttgccggcagcgacaaccttcccgaactcaccggactggcgatcggcgccctgatcggccgctctgcggccgatgtcttcgactcggagacgcacaaccgtctgacgatcgccttggccgagcccggggcggccgtcggagcaccgatcactgtcggcttcacgatgcgaaaggacgcaggcttcatcggctcctggcatcgccatgatcagctcatcttcctcgagctcgagcctccccagcgggacgtcgccgagccgcaggcgttcttccgccgcaccaacagcgccatccgccgcctacaagccgccgaaaccttggaaagcgcctgcgccgccgcggcgcaagaggtgcggaagattaccggcttcgatcgggtgatgatctatcgcttcgcctccgacttcagcggcgaagtgatcgcagaggatcggtgcgccgaggtcgagtcaaaactaggcctgcactatcctgcctcaaccgtgccggcgcaggcccgtcggctctataccatcaacccggtacggatcattcccgatatcaattatcggccggtgccggtcaccccagacctcaatccggtcaccgggcggccgattgatcttagcttcgccatcctgcgcagcgtctcgcccgtccatctggagtttatgcgcaacataggcatgcacggcacgatgtcgatctcgattttgcgcggcgagcgactgtggggattgatcgtttgccatcaccgaacgccgtactacgtcgatctcgatggccgccaagcctgcgagctagtcgcccaggttctggcctggcagatcggcgtgatggaagagtga BBa_K1878001_sequence 1 atgacagaaggatccgtcgccaggcagcctgacctcttgacctgcgacgatgagccgatccatatccccggtgccatccaaccgcatggactgctgctcgccctcgccgccgacatgacgatcgttgccggcagcgacaaccttcccgaactcaccggactggcgatcggcgccctgatcggccgctctgcggccgatgtcttcgactcggagacgcacaaccgtctgacgatcgccttggccgagcccggggcggccgtcggagcaccgatcactgtcggcttcacgatgcgaaaggacgcaggcttcatcggctcctggcatcgccatgatcagctcatcttcctcgagctcgagcctccccagcgggacgtcgccgagccgcaggcgttcttccgccgcaccaacagcgccatccgccgcctacaagccgccgaaaccttggaaagcgcctgcgccgccgcggcgcaagaggtgcggaagattaccggcttcgatcgggtgatgatctatcgcttcgcctccgacttcagcggcgaagtgatcgcagaggatcggtgcgccgaggtcgagtcaaaactaggcctgcactatcctgcctcaaccgtgccggcgcaggcccgtcggctctataccatcaacccggtacggatcattcccgatatcaattatcggccggtgccggtcaccccagacctcaatccggtcaccgggcggccgattgatcttagcttcgccatcctgcgcagcgtctcgcccgtccatctggagtttatgcgcaacataggcatgcacggcacgatgtcgatctcgattttgcgcggcgagcgactgtggggattgatcgtttgccatcaccgaacgccgtactacgtcgatctcgatggccgccaagcctgcgagctagtcgcccaggttctggcctggcagatcggcgtgatggaagagtga BBa_K239006_sequence 1 gtattgataaatatcaatgatagataaagttatcttatcgtttgatttacatcaaattgcctttagctacagacactaaggtggcagac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z