LOCUS BBa_K188000 2124 bp DNA linear 28-Jun-2024 DEFINITION The device for regulation between lactose and promoter Lac. ACCESSION BBa_K188000 VERSION BBa_K188000.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23106 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 misc_feature 1151..1189 /label=LVA CDS 62..1189 /label=lacI-LVA misc_feature 62..1189 /label=BBa_C0012 misc_feature 1190..1214 /label=Help:Barcodes CDS 62..1189 /label=BBa_C0012 misc_feature 1227..1230 /label=conserved RBS 1223..1234 /label=BBa_B0034 misc_feature 1241..1242 /label=prefix misc_feature 1414..1414 /label=T misc_feature 1732..1732 /label=A CDS 1241..1990 /label=luxR misc_feature 1241..1996 /label=BBa_C0062 misc_feature 1997..2021 /label=Help:Barcodes CDS 1241..1996 /label=BBa_C0062 stem_loop 2037..2056 /label=T7 TE misc_feature 2063..2063 /label=stop polyA_site 2057..2070 /label=polya misc_feature 2030..2070 /label=BBa_B0012 terminator 2030..2070 /label=BBa_B0012 stem_loop 2091..2113 /label=stem_loop misc_feature 2079..2124 /label=BBa_B0011 terminator 2079..2124 /label=BBa_B0011 terminator 2030..2124 /label=BBa_B0014 ORIGIN 1 tttacggcta gctcagtcct aggtatagtg ctagctacta gagaaagagg agaaatacta 61 gatggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta 121 tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa 181 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc 241 gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc 301 gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc 361 gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca 421 acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga 481 agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa 541 cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt 601 gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg 661 tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga 721 aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat 781 cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat 841 taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga 901 agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg 961 gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca 1021 gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc 1081 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga 1141 aagcgggcag gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg 1201 ctagtgtaga tctctactag agaaagagga gaaatactag atgaaaaaca taaatgccga 1261 cgacacatac agaataatta ataaaattaa agcttgtaga agcaataatg atattaatca 1321 atgcttatct gatatgacta aaatggtaca ttgtgaatat tatttactcg cgatcattta 1381 tcctcattct atggttaaat ctgatatttc aatcctagat aattacccta aaaaatggag 1441 gcaatattat gatgacgcta atttaataaa atatgatcct atagtagatt attctaactc 1501 caatcattca ccaattaatt ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc 1561 aaatgtaatt aaagaagcga aaacatcagg tcttatcact gggtttagtt tccctattca 1621 tacggctaac aatggcttcg gaatgcttag ttttgcacat tcagaaaaag acaactatat 1681 agatagttta tttttacatg cgtgtatgaa cataccatta attgttcctt ctctagttga 1741 taattatcga aaaataaata tagcaaataa taaatcaaac aacgatttaa ccaaaagaga 1801 aaaagaatgt ttagcgtggg catgcgaagg aaaaagctct tgggatattt caaaaatatt 1861 aggttgcagt gagcgtactg tcactttcca tttaaccaat gcgcaaatga aactcaatac 1921 aacaaaccgc tgccaaagta tttctaaagc aattttaaca ggagcaattg attgcccata 1981 ctttaaaaat taataacact gatagtgcta gtgtagatca ctactagagt cacactggct 2041 caccttcggg tgggcctttc tgcgtttata tactagagag agaatataaa aagccagatt 2101 attaatccgg cttttttatt attt //