LOCUS       BBa_K188004             1557 bp    DNA     linear       14-Jun-2024
DEFINITION  fsfa
ACCESSION   BBa_K188004
VERSION     BBa_K188004.1  
FEATURES             Location/Qualifiers
     misc_feature    1..54
                     /label=BBa_R0011
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    56..67
                     /label=BBa_B0034
     misc_feature    60..63
                     /label=conserved
     CDS             68..736
                     /label=cI 434
     misc_feature    68..736
                     /label=BBa_C0052
     misc_feature    698..736
                     /label=LVA
     misc_feature    737..761
                     /label=barcode
     misc_feature    762..773
                     /label=BBa_B0034
     misc_feature    766..769
                     /label=conserved
     misc_feature    774..1433
                     /label=BBa_C0040
     CDS             777..1393
                     /label=tetR
     misc_feature    1394..1427
                     /label=SsrA
     misc_feature    1434..1458
                     /label=barcode
     misc_feature    1459..1470
                     /label=BBa_B0034
     misc_feature    1463..1466
                     /label=conserved
     misc_feature    1471..1511
                     /label=BBa_B0012
     stem_loop       1478..1497
                     /label=T7 TE
     polyA_site      1498..1511
                     /label=polya
     misc_feature    1504..1504
                     /label=stop
     misc_feature    1512..1557
                     /label=BBa_B0011
     stem_loop       1524..1546
                     /label=stem_loop
     misc_feature    1..1557
                     /label=BBa_K188004
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacaaaaga
       61 ggagaaaatg agtatttctt ccagggtaaa aagcaaaaga atccagcttg gacttaacca
      121 ggctgaactt gctcaaaagg tggggactac ccagcagtct atagagcagc tcgaaaacgg
      181 taaaactaag cgaccacgct ttttaccaga acttgcgtca gctcttggcg taagtgttga
      241 ctggctgctc aatggcacct ctgattcgaa tgttagattt gttgggcacg ttgagcccaa
      301 agggaaatat ccattgatta gcatggttag agctggttcg tggtgtgaag cttgtgaacc
      361 ctacgatatc aaggacattg atgaatggta tgacagtgac gttaacttat taggcaatgg
      421 attctggctg aaggttgaag gtgattccat gacctcacct gtaggtcaaa gcatccctga
      481 aggtcatatg gtgttagtag atactggacg ggagccagtg aatggaagcc ttgttgtagc
      541 caaactgact gacgcgaacg aagcaacatt caagaaactg gtcatagatg gcggtcagaa
      601 gtacctgaaa ggcctgaatc cttcatggcc tatgactcct atcaacggaa actgcaagat
      661 tatcggtgtt gtcgtggaag cgagggtaaa attcgtagct gcaaacgacg aaaactacgc
      721 tttagtagct taataactct gatagtgcta gtgtagatct caaagaggag aaaatgtcca
      781 gattagataa aagtaaagtg attaacagcg cattagagct gcttaatgag gtcggaatcg
      841 aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt agagcagcct acattgtatt
      901 ggcatgtaaa aaataagcgg gctttgctcg acgccttagc cattgagatg ttagataggc
      961 accatactca cttttgccct ttagaagggg aaagctggca agatttttta cgtaataacg
     1021 ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg agcaaaagta catttaggta
     1081 cacggcctac agaaaaacag tatgaaactc tcgaaaatca attagccttt ttatgccaac
     1141 aaggtttttc actagagaat gcattatatg cactcagcgc tgtggggcat tttactttag
     1201 gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa acacctacta
     1261 ctgatagtat gccgccatta ttacgacaag ctatcgaatt atttgatcac caaggtgcag
     1321 agccagcctt cttattcggc cttgaattga tcatatgcgg attagaaaaa caacttaaat
     1381 gtgaaagtgg gtccgctgca aacgacgaaa actacgcttt agtagcttaa taacactgat
     1441 agtgctagtg tagatcacaa agaggagaaa tcacactggc tcaccttcgg gtgggccttt
     1501 ctgcgtttat aagagaatat aaaaagccag attattaatc cggctttttt attattt
//