##gff-version 3
##sequence-region BBa_K188004 1 1557
BBa_K188004	.	promoter	43	48	.	+	0	ID=annotation2039850;Name=-10
BBa_K188004	.	CDS	68	736	.	+	0	ID=annotation2039853;Name=cI 434
BBa_K188004	.	engineered_region	1	54	.	+	0	ID=annotation2039846;Name=BBa_R0011
BBa_K188004	.	engineered_region	737	761	.	+	0	ID=annotation2039856;Name=barcode
BBa_K188004	.	engineered_region	56	67	.	+	0	ID=annotation2039851;Name=BBa_B0034
BBa_K188004	.	engineered_region	774	1433	.	+	0	ID=annotation2039859;Name=BBa_C0040
BBa_K188004	.	engineered_region	1434	1458	.	+	0	ID=annotation2039862;Name=barcode
BBa_K188004	.	conserved_region	60	63	.	+	0	ID=annotation2039852;Name=conserved
BBa_K188004	.	conserved_region	1463	1466	.	+	0	ID=annotation2039864;Name=conserved
BBa_K188004	.	engineered_region	1	1557	.	+	0	ID=annotation2039871;Name=BBa_K188004
BBa_K188004	.	engineered_region	68	736	.	+	0	ID=annotation2039854;Name=BBa_C0052
BBa_K188004	.	CDS	777	1393	.	+	0	ID=annotation2039860;Name=tetR
BBa_K188004	.	polyA_site	1498	1511	.	+	0	ID=annotation2039867;Name=polya
BBa_K188004	.	stem_loop	1524	1546	.	+	0	ID=annotation2039870;Name=stem_loop
BBa_K188004	.	operator	3	19	.	+	0	ID=annotation2039847;Name=lac O1
BBa_K188004	.	conserved_region	766	769	.	+	0	ID=annotation2039858;Name=conserved
BBa_K188004	.	tag	1394	1427	.	+	0	ID=annotation2039861;Name=SsrA
BBa_K188004	.	engineered_region	1459	1470	.	+	0	ID=annotation2039863;Name=BBa_B0034
BBa_K188004	.	stem_loop	1478	1497	.	+	0	ID=annotation2039866;Name=T7 TE
BBa_K188004	.	engineered_region	762	773	.	+	0	ID=annotation2039857;Name=BBa_B0034
BBa_K188004	.	operator	26	42	.	+	0	ID=annotation2039849;Name=lac O1
BBa_K188004	.	engineered_region	1471	1511	.	+	0	ID=annotation2039865;Name=BBa_B0012
BBa_K188004	.	promoter	20	25	.	+	0	ID=annotation2039848;Name=-35
BBa_K188004	.	tag	698	736	.	+	0	ID=annotation2039855;Name=LVA
BBa_K188004	.	engineered_region	1512	1557	.	+	0	ID=annotation2039869;Name=BBa_B0011
BBa_K188004	.	stop_codon	1504	1504	.	+	0	ID=annotation2039868;Name=stop
>BBa_K188004
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacaaaagaggagaaaatgagtatttct
tccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagt
ctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgt
tgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattg
attagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatgaatggtatgaca
gtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacctgtaggtcaaagcat
ccctgaaggtcatatggtgttagtagatactggacgggagccagtgaatggaagccttgttgtagccaaactgactgac
gcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagtacctgaaaggcctgaatccttcatggccta
tgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagcgagggtaaaattcgtagctgcaaacgacga
aaactacgctttagtagcttaataactctgatagtgctagtgtagatctcaaagaggagaaaatgtccagattagataa
aagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccag
aagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgaga
tgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaag
ttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaa
actctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtgg
ggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactga
tagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggcctt
gaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttag
tagcttaataacactgatagtgctagtgtagatcacaaagaggagaaatcacactggctcaccttcgggtgggcctttc
tgcgtttataagagaatataaaaagccagattattaatccggcttttttattattt