LOCUS       BBa_K188006             1233 bp    DNA     linear       22-Nov-2024
DEFINITION  Expression of ccdB for self-destruction of bacteria. Since 
            promoter lux/cIIp22, this sequence can be
ACCESSION   BBa_K188006
VERSION     BBa_K188006.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=Lux-box
     promoter        20..25
                     /label=-35
     misc_feature    23..40
                     /label=OR2
     promoter        42..47
                     /label=-10
     misc_feature    46..63
                     /label=OR1
     promoter        1..66
                     /label=BBa_K145150
     misc_feature    79..82
                     /label=conserved
     RBS             75..86
                     /label=BBa_B0034
     misc_feature    93..95
                     /label=start
     misc_feature    396..398
                     /label=stop
     CDS             93..395
                     /label=cds
     CDS             93..398
                     /label=BBa_K145151
     misc_feature    411..414
                     /label=conserved
     RBS             407..418
                     /label=BBa_B0034
     CDS             425..1099
                     /label=mrfp1
     misc_feature    1106..1130
                     /label=Help:Barcodes
     CDS             425..1130
                     /label=BBa_E1010
     stem_loop       1146..1165
                     /label=T7 TE
     misc_feature    1172..1172
                     /label=stop
     polyA_site      1166..1179
                     /label=polya
     misc_feature    1139..1179
                     /label=BBa_B0012
     terminator      1139..1179
                     /label=BBa_B0012
     stem_loop       1200..1222
                     /label=stem_loop
     misc_feature    1188..1233
                     /label=BBa_B0011
     terminator      1188..1233
                     /label=BBa_B0011
     terminator      1139..1233
                     /label=BBa_B0014
ORIGIN
        1 acctgtagga tcgtacaggt ttactaaaga ttcctttagt ttataattta agtgttcttt
       61 aatttctact agagaaagag gagaaatact agatgcagtt taaggtttac acctataaaa
      121 gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac acgcccgggc
      181 gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc tcccgtgaac
      241 tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca
      301 gtgtgccggt ctccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca
      361 tcaaaaacgc cattaacctg atgttctggg gaatataata ctagagaaag aggagaaata
      421 ctagatggct tcctccgaag acgttatcaa agagttcatg cgtttcaaag ttcgtatgga
      481 aggttccgtt aacggtcacg agttcgaaat cgaaggtgaa ggtgaaggtc gtccgtacga
      541 aggtacccag accgctaaac tgaaagttac caaaggtggt ccgctgccgt tcgcttggga
      601 catcctgtcc ccgcagttcc agtacggttc caaagcttac gttaaacacc cggctgacat
      661 cccggactac ctgaaactgt ccttcccgga aggtttcaaa tgggaacgtg ttatgaactt
      721 cgaagacggt ggtgttgtta ccgttaccca ggactcctcc ctgcaagacg gtgagttcat
      781 ctacaaagtt aaactgcgtg gtaccaactt cccgtccgac ggtccggtta tgcagaaaaa
      841 aaccatgggt tgggaagctt ccaccgaacg tatgtacccg gaagacggtg ctctgaaagg
      901 tgaaatcaaa atgcgtctga aactgaaaga cggtggtcac tacgacgctg aagttaaaac
      961 cacctacatg gctaaaaaac cggttcagct gccgggtgct tacaaaaccg acatcaaact
     1021 ggacatcacc tcccacaacg aagactacac catcgttgaa cagtacgaac gtgctgaagg
     1081 tcgtcactcc accggtgctt aataacgctg atagtgctag tgtagatcgc tactagagtc
     1141 acactggctc accttcgggt gggcctttct gcgtttatat actagagaga gaatataaaa
     1201 agccagatta ttaatccggc ttttttatta ttt
//