LOCUS       BBa_K188009             2324 bp    DNA     linear       10-Dec-2025
DEFINITION  sa
ACCESSION   BBa_K188009
VERSION     BBa_K188009.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    60..63
                     /label=conserved
     RBS             56..67
                     /label=BBa_B0034
     CDS             68..736
                     /label=cI 434
     misc_feature    698..736
                     /label=LVA
     misc_feature    68..736
                     /label=BBa_C0052
     misc_feature    737..761
                     /label=Help:Barcodes
     CDS             68..736
                     /label=BBa_C0052
     misc_feature    766..769
                     /label=conserved
     RBS             762..773
                     /label=BBa_B0034
     CDS             777..1393
                     /label=tetR
     misc_feature    1394..1427
                     /label=SsrA
     misc_feature    1434..1458
                     /label=Help:Barcodes
     CDS             774..1458
                     /label=BBa_C0040
     misc_feature    1463..1466
                     /label=conserved
     RBS             1459..1470
                     /label=BBa_B0034
     misc_feature    1471..1473
                     /label=start
     misc_feature    2224..2226
                     /label=stop
     misc_feature    2227..2229
                     /label=stop
     misc_feature    2187..2223
                     /label=LVA tag
     CDS             1474..2186
                     /label=cds
     misc_feature    1471..2229
                     /label=BBa_K145015
     stem_loop       2237..2256
                     /label=T7 TE
     misc_feature    2263..2263
                     /label=stop
     polyA_site      2257..2270
                     /label=polya
     misc_feature    2230..2270
                     /label=BBa_B0012
     terminator      2230..2270
                     /label=BBa_B0012
     stem_loop       2291..2313
                     /label=stem_loop
     misc_feature    2279..2324
                     /label=BBa_B0011
     terminator      2279..2324
                     /label=BBa_B0011
     terminator      2230..2324
                     /label=BBa_B0014
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacaaaaga
       61 ggagaaaatg agtatttctt ccagggtaaa aagcaaaaga atccagcttg gacttaacca
      121 ggctgaactt gctcaaaagg tggggactac ccagcagtct atagagcagc tcgaaaacgg
      181 taaaactaag cgaccacgct ttttaccaga acttgcgtca gctcttggcg taagtgttga
      241 ctggctgctc aatggcacct ctgattcgaa tgttagattt gttgggcacg ttgagcccaa
      301 agggaaatat ccattgatta gcatggttag agctggttcg tggtgtgaag cttgtgaacc
      361 ctacgatatc aaggacattg atgaatggta tgacagtgac gttaacttat taggcaatgg
      421 attctggctg aaggttgaag gtgattccat gacctcacct gtaggtcaaa gcatccctga
      481 aggtcatatg gtgttagtag atactggacg ggagccagtg aatggaagcc ttgttgtagc
      541 caaactgact gacgcgaacg aagcaacatt caagaaactg gtcatagatg gcggtcagaa
      601 gtacctgaaa ggcctgaatc cttcatggcc tatgactcct atcaacggaa actgcaagat
      661 tatcggtgtt gtcgtggaag cgagggtaaa attcgtagct gcaaacgacg aaaactacgc
      721 tttagtagct taataactct gatagtgcta gtgtagatct caaagaggag aaaatgtcca
      781 gattagataa aagtaaagtg attaacagcg cattagagct gcttaatgag gtcggaatcg
      841 aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt agagcagcct acattgtatt
      901 ggcatgtaaa aaataagcgg gctttgctcg acgccttagc cattgagatg ttagataggc
      961 accatactca cttttgccct ttagaagggg aaagctggca agatttttta cgtaataacg
     1021 ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg agcaaaagta catttaggta
     1081 cacggcctac agaaaaacag tatgaaactc tcgaaaatca attagccttt ttatgccaac
     1141 aaggtttttc actagagaat gcattatatg cactcagcgc tgtggggcat tttactttag
     1201 gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa acacctacta
     1261 ctgatagtat gccgccatta ttacgacaag ctatcgaatt atttgatcac caaggtgcag
     1321 agccagcctt cttattcggc cttgaattga tcatatgcgg attagaaaaa caacttaaat
     1381 gtgaaagtgg gtccgctgca aacgacgaaa actacgcttt agtagcttaa taacactgat
     1441 agtgctagtg tagatcacaa agaggagaaa atgcgtaaag gagaagaact tttcactgga
     1501 gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt
     1561 ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact
     1621 ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc
     1681 tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa
     1741 ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct
     1801 gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt
     1861 aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc acacaatgta
     1921 tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac
     1981 attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat
     2041 ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat
     2101 cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca
     2161 catggcatgg atgaactata caaaaggcct gctgcaaacg acgaaaacta cgctttagta
     2221 gcttaataat cacactggct caccttcggg tgggcctttc tgcgtttata tactagagag
     2281 agaatataaa aagccagatt attaatccgg cttttttatt attt
//