LOCUS       BBa_K188244              961 bp    DNA     linear       22-Dec-2024
DEFINITION  Promoter (lacI regulated, lambda pL hybrid)+RBS+GFP-LVA+terminator
ACCESSION   BBa_K188244
VERSION     BBa_K188244.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    82..84
                     /label=start
     misc_feature    835..837
                     /label=stop
     misc_feature    838..840
                     /label=stop
     misc_feature    798..834
                     /label=LVA tag
     CDS             85..797
                     /label=cds
     misc_feature    82..840
                     /label=BBa_K145015
     terminator      849..961
                     /label=BBa_J61048
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca
      121 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt
      181 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta
      241 cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga
      301 tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta
      361 caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag
      421 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat
      481 ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt atacatcatg
      541 gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat
      601 ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc
      661 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa
      721 aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg
      781 gatgaactat acaaaaggcc tgctgcaaac gacgaaaact acgctttagt agcttaataa
      841 tactagagcc ggcttatcgg tcagtttcac ctgatttacg taaaaacccg cttcggcggg
      901 tttttgcttt tggaggggca gaaagatgaa tgactgtcca cgacgctata cccaaaagaa
      961 a
//