LOCUS       BBa_K188261             1681 bp    DNA     linear       04-May-2025
DEFINITION  Plac+RBS+CI434+RBS+GFP-LVA+terminator
ACCESSION   BBa_K188261
VERSION     BBa_K188261.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..750
                     /label=cI 434
     misc_feature    712..750
                     /label=LVA
     misc_feature    82..750
                     /label=BBa_C0052
     misc_feature    751..775
                     /label=Help:Barcodes
     CDS             82..750
                     /label=BBa_C0052
     misc_feature    788..791
                     /label=conserved
     RBS             784..795
                     /label=BBa_B0034
     misc_feature    802..804
                     /label=start
     misc_feature    1555..1557
                     /label=stop
     misc_feature    1558..1560
                     /label=stop
     misc_feature    1518..1554
                     /label=LVA tag
     CDS             805..1517
                     /label=cds
     misc_feature    802..1560
                     /label=BBa_K145015
     terminator      1569..1681
                     /label=BBa_J61048
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgagtatt tcttccaggg taaaaagcaa aagaatccag
      121 cttggactta accaggctga acttgctcaa aaggtgggga ctacccagca gtctatagag
      181 cagctcgaaa acggtaaaac taagcgacca cgctttttac cagaacttgc gtcagctctt
      241 ggcgtaagtg ttgactggct gctcaatggc acctctgatt cgaatgttag atttgttggg
      301 cacgttgagc ccaaagggaa atatccattg attagcatgg ttagagctgg ttcgtggtgt
      361 gaagcttgtg aaccctacga tatcaaggac attgatgaat ggtatgacag tgacgttaac
      421 ttattaggca atggattctg gctgaaggtt gaaggtgatt ccatgacctc acctgtaggt
      481 caaagcatcc ctgaaggtca tatggtgtta gtagatactg gacgggagcc agtgaatgga
      541 agccttgttg tagccaaact gactgacgcg aacgaagcaa cattcaagaa actggtcata
      601 gatggcggtc agaagtacct gaaaggcctg aatccttcat ggcctatgac tcctatcaac
      661 ggaaactgca agattatcgg tgttgtcgtg gaagcgaggg taaaattcgt agctgcaaac
      721 gacgaaaact acgctttagt agcttaataa ctctgatagt gctagtgtag atctctacta
      781 gagaaagagg agaaatacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca
      841 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt
      901 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta
      961 cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga
     1021 tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta
     1081 caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag
     1141 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat
     1201 ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt atacatcatg
     1261 gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat
     1321 ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc
     1381 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa
     1441 aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg
     1501 gatgaactat acaaaaggcc tgctgcaaac gacgaaaact acgctttagt agcttaataa
     1561 tactagagcc ggcttatcgg tcagtttcac ctgatttacg taaaaacccg cttcggcggg
     1621 tttttgcttt tggaggggca gaaagatgaa tgactgtcca cgacgctata cccaaaagaa
     1681 a
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