##gff-version 3
##sequence-region BBa_K188281 1 2392
BBa_K188281	.	CDS	82	750	.	+	0	ID=BBa_C0052;Name=BBa_C0052
BBa_K188281	.	engineered_tag	751	775	.	+	0	ID=annotation2213992;Name=Help:Barcodes;Parent=BBa_C0052
BBa_K188281	.	engineered_region	82	750	.	+	0	ID=annotation7035;Name=BBa_C0052;Parent=BBa_C0052
BBa_K188281	.	CDS	82	750	.	+	0	ID=annotation1743;Name=cI 434;Parent=BBa_C0052
BBa_K188281	.	tag	712	750	.	+	0	ID=annotation1745;Name=LVA;Parent=BBa_C0052
BBa_K188281	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K188281	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K188281	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K188281	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K188281	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K188281	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K188281	.	CDS	802	1486	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_K188281	.	engineered_tag	1462	1486	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K188281	.	tag	1422	1455	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K188281	.	CDS	805	1421	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K188281	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K188281	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K188281	.	terminator	2280	2392	.	+	0	ID=BBa_J61048;Name=BBa_J61048
BBa_K188281	.	engineered_region	1513	2271	.	+	0	ID=BBa_K145015;Name=BBa_K145015
BBa_K188281	.	stop_codon	2266	2268	.	+	0	ID=annotation1969617;Name=stop;Parent=BBa_K145015
BBa_K188281	.	start_codon	1513	1515	.	+	0	ID=annotation1969616;Name=start;Parent=BBa_K145015
BBa_K188281	.	CDS	1516	2228	.	+	0	ID=annotation1969620;Name=cds;Parent=BBa_K145015
BBa_K188281	.	stop_codon	2269	2271	.	+	0	ID=annotation1969618;Name=stop;Parent=BBa_K145015
BBa_K188281	.	tag	2229	2265	.	+	0	ID=annotation1969619;Name=LVA tag;Parent=BBa_K145015
BBa_K188281	.	ribosome_entry_site	784	795	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K188281	.	conserved_region	788	791	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K188281	.	ribosome_entry_site	1495	1506	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K188281	.	conserved_region	1499	1502	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K188281
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggg
gactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagct
cttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaag
ggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattga
tgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacct
gtaggtcaaagcatccctgaaggtcatatggtgttagtagatactggacgggagccagtgaatggaagccttgttgtag
ccaaactgactgacgcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagtacctgaaaggcctgaa
tccttcatggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagcgagggtaaaattcgta
gctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaagagg
agaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcga
aggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgg
gctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggc
aagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttagg
tacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagag
aatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgcta
aagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaagg
tgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtcc
gctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagagg
agaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaa
tgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcact
actggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccag
atcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaaga
tgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtatt
gattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcag
acaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagacca
ttatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctt
tcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatgg
atgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccggcttatcgg
tcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtcc
acgacgctatacccaaaagaaa