##gff-version 3
##sequence-region BBa_K188343 1 942
BBa_K188343	.	terminator	848	888	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K188343	.	polyA_site	875	888	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K188343	.	stem_loop	855	874	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K188343	.	stop_codon	881	881	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K188343	.	engineered_region	848	888	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K188343	.	engineered_region	81	839	.	+	0	ID=BBa_K145015;Name=BBa_K145015
BBa_K188343	.	start_codon	81	83	.	+	0	ID=annotation1969616;Name=start;Parent=BBa_K145015
BBa_K188343	.	CDS	84	796	.	+	0	ID=annotation1969620;Name=cds;Parent=BBa_K145015
BBa_K188343	.	stop_codon	834	836	.	+	0	ID=annotation1969617;Name=stop;Parent=BBa_K145015
BBa_K188343	.	tag	797	833	.	+	0	ID=annotation1969619;Name=LVA tag;Parent=BBa_K145015
BBa_K188343	.	stop_codon	837	839	.	+	0	ID=annotation1969618;Name=stop;Parent=BBa_K145015
BBa_K188343	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K188343	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K188343	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K188343	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K188343	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K188343	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K188343	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K188343	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K188343	.	terminator	897	942	.	+	0	ID=BBa_B0011;Name=BBa_B0011
BBa_K188343	.	engineered_region	897	942	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K188343	.	stem_loop	909	931	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
>BBa_K188343
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaa
ttttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaac
tacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaa
acagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaac
tacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaag
aagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaa
gaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaa
aatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatc
ccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactata
caaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagtcacactggctcaccttcgggt
gggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt