BBa_K1884005
1
D1
PsbA
2016-10-08T11:00:00Z
2016-10-19T05:06:35Z
The DNA sequence of D1 protein is designed by ourselves.
This family represents the D1 protein (also known as PsbA), which forms the reaction core of PSII as a heterodimer with the D2 protein. In higher plants, the N-terminal residues of both proteins, which are exposed to the stromal surface, can be reversibly phosphorylated. After insertion in the membrane, the C-terminal of the D1 protein is cleaved by a C-terminal processing protease to yield the mature protein . This processing is essential for the assembly of a functional 4-atom manganese cluster, which involves binding to a highly conserved C-terminal alanine 344. The Mn cluster is located on the lumenal surface of the D1 and D2 proteins. In addition to the Mn cluster, the D1/D2 core binds to a number of cofactors, including two pheophytin molecules, only one of which is phytochemically active; non-haem iron; and two quinones, Qa (bound to D2) and Qb (bound to D1). Upon light excitation, an electron is transferred from the primary donor (chlorophyll a) via intermediate acceptor pheophytin to the primary quinone Qa, then to the secondary quinone Qb. At the oxidising side of PSII, a redox-active residue in the D1 protein reduces P680, the oxidised tyrosine then withdrawing electrons from a manganese cluster, which in turn withdraw electrons from water, leading to the splitting of water and the formation of molecular oxygen. PSII thus provides a source of electrons that can be used by photosystem I to produce the reducing power (NADPH) required to convert CO2 to glucose.
false
false
_2349_
29275
29275
9
false
false
Changxing Hu
BBa_K509003
1
BBa_K509003
PSAD Terminator
2011-09-18T11:00:00Z
2015-05-08T01:12:30Z
plasmid
PSAD Terminator
false
false
_672_
0
8421
9
Not in stock
false
to edit
false
Ben Jevans
BBa_K1884004
1
Gal4-UAS
Upstream activating sequence
2016-10-06T11:00:00Z
2016-10-15T07:42:46Z
PhyB (photochrome B)
PhyB is red/far-red photoreceptor involved in the regulation of de-etiolation. It is involved in the light-promotion of seed germination and in the shade avoidance response.
false
false
_2349_
29275
30234
9
No part sequence
false
PhyB functions as a transcriptional activator, and can be functionally and mechanistically separated from its role in repression of PIF3 mediated processes.
false
Chengrong Xie
annotation2489054
1
UAS
range2489054
1
1
179
BBa_K1884008
1
BBa_K1884008
PSAD Promoter+GalAD-CRY2+UAS+PsbA+PSAD Terminator
2016-10-10T11:00:00Z
2016-10-19T05:47:09Z
false
false
_2349_
29275
29275
9
false
false
Changxing Hu
component2492798
1
BBa_K1884002
component2492800
1
BBa_K1884004
component2492797
1
BBa_K1547005
component2492802
1
BBa_K509003
component2492801
1
BBa_K1884005
annotation2492802
1
BBa_K509003
range2492802
1
3351
3883
annotation2492797
1
BBa_K1547005
range2492797
1
1
894
annotation2492800
1
BBa_K1884004
range2492800
1
3015
3193
annotation2492798
1
BBa_K1884002
range2492798
1
901
3006
annotation2492801
1
BBa_K1884005
range2492801
1
3202
3342
BBa_K1884002
1
AD-CRY2
VP16AD-CRY2
2016-10-06T11:00:00Z
2016-10-13T08:55:18Z
Part cloned from pRMH120 of Addgene
Cryptochrome 2 (CRY2) is a blue light stimulated photoreceptor, when exposed to blue light, it would interact with CIB1. This part is a Gal4 DNA activating domain fused to N terminus of CRY2.
false
false
_2349_
29275
30234
9
Not in stock
false
One powerful application of protein-interaction dimerizers(CRY2-CIB1) is to allow inducible control of a 'split' protein, in which two inactive fragments are brought together to reconstitute a functional protein activity. In principle, using this approach with optically controlled dimerizers would confer light-dependent activity to a diverse range of target proteins. We used the CRY2-CIB1 modules to reconstitute split versions of Gal4 transcription factor proteins. Based on the transient nature of the CRY-CIB interaction,the light pulses would allow precise, dose-dependent control of protein activity. We expressed in yeast the split Gal4 partners Gal4BD- CIB1 and Gal4AD-CRY2 together with upstream activating sequence under control of a galactose-inducible promoter. We incubated the yeast in the dark or subjected them to up to twenty blue-light pulses over 4 h. Immunoblot analysis of Snl1 revealed a strong dose dependence of protein expression in response to light.
false
Chengrong Xie
BBa_K1547005
1
BBa_K1547005
PSAD Promoter
2014-10-07T11:00:00Z
2015-05-08T01:10:53Z
Chlamydomonas reinhardtii nucleus
This is a plant promoter from the green algae Chlamydomonas reinhardtii.
false
false
_1931_
0
22390
9
It's complicated
false
PSAD was amplified using PCR on a plasmid containing the promoter supplied by chlamycollection.org
false
victor yuan
BBa_K1884002_sequence
1
atgggccccaagaagaagcgcaaggtggccccccccaccgacgtgagcctgggcgacgagctgcacctggacggcgaggacgtggcgatggcccacgccgacgccctggacgacttcgacctggacatgctgggcgacggcgacagccccggccccggcttcaccccccacgacagcgccccctacggcgccctggacatggccgacttcgagttcgagcagatgttcaccgacgccctgggcatcgacgagtacggcggccatatgatgaagatggacaaaaagactatagtttggtttagaagagacctaaggattgaggataatcctgcattagcagcagctgctcacgaaggatctgtttttcctgtcttcatttggtgtcctgaagaagaaggacagttttatcctggaagagcttcaagatggtggatgaaacaatcacttgctcacttatctcaatccttgaaggctcttggatctgacctcactttaatcaaaacccacaacacgatttcagcgatcttggattgtatccgcgttaccggtgctacaaaagtcgtctttaaccacctctatgatcctgtttcgttagttcgggaccataccgtaaaggagaagctggtggaacgtgggatctctgtgcaaagctacaatggagatctattgtatgaaccgtgggagatatactgcgaaaagggcaaaccttttacgagtttcaattcttactggaagaaatgcttagatatgtcgattgaatccgttatgcttcctcctccttggcggttgatgccaataactgcggcggctgaagcgatttgggcgtgttcgattgaagaactagggctggagaatgaggccgagaaaccgagcaatgcgttgttaactagagcttggtctccaggatggagcaatgctgataagttactaaatgagttcatcgagaagcagttgatagattatgcaaagaacagcaagaaagttgttgggaactctacttcactactttctccgtatctccatttcggggaaataagcgtcagacacgttttccagtgtgcccggatgaaacaaattatatgggcaagagataagaacagtgaaggagaagaaagtgcagatctttttcttaggggaatcggtttaagagagtattctcggtatatatgtttcaacttcccgtttactcacgagcaatcgttgttgagtcatcttcggtttttcccttgggatgctgatgttgataagttcaaggcctggagacaaggcaggaccggttatccgttggtggatgccggaatgagagagctttgggctaccggatggatgcataacagaataagagtgattgtttcaagctttgctgtgaagtttcttctccttccatggaaatggggaatgaagtatttctgggatacacttttggatgctgatttggaatgtgacatccttggctggcagtatatctctgggagtatccccgatggccacgagcttgatcgcttggacaatcccgcgttacaaggcgccaaatatgacccagaaggtgagtacataaggcaatggcttcccgagcttgcgagattgccaactgaatggatccatcatccatgggacgctcctttaaccgtactcaaagcttctggtgtggaactcggaacaaactatgcgaaacccattgtagacatcgacacagctcgtgagctactagctaaagctatttcaagaacccgtgaagcacagatcatgatcggagcagcacctgatgagattgtagcagatagcttcgaggccttaggggctaataccattaaagaacctggtctttgcccatctgtgtcttctaatgaccaacaagtaccttcggctgttcgttacaacgggtcaaagagagtgaaacctgaggaagaagaagagagagacatgaagaaatctaggggattcgatgaaagggagttgttttcgactgctgaatcttcttcttcttcgagtgtgtttttcgtttcgcagtcttgctcgttggcatcagaagggaagaatctggaaggtattcaagattcatctgatcagattactacaagtttgggaaaaaatggttgcaaatga
BBa_K1884005_sequence
1
gcggggccctgacaccactgcggccgcctactcggtggttcattaacgccgcgcctggacccgagggaggacccctcgggacccggtacgtcgttaatgaaccaccgaatacgcggtcgcggtggggtcaggtccttccgc
BBa_K1547005_sequence
1
taattcatcaaattataactatatagaccctaatttcatacacacacctgcccgtctgcctgacaggaagtgaacgcatgtcgagggaggcctcaccaatcgtcacacgagccctcgtcagaaacacgtctccgccacgctctccctctcacggccgaccccgcagcccttttgccctttcctaggccaccgacaggacccaggcgctctcagcatgcctcaacaacccgtactcgtgccagcggtgcccttgtgctggtgatcgcttggaagcgcatgcgaagacgaaggggcggagcaggcggcctggctgttcgaagggctcgccgccagttcgggtgcctttctccacgcgcgcctccacacctaccgatgcgtgaaggcaggcaaatgctcatgtttgcccgaactcggagtccttaaaaagccgcttcttgtcgtcgttccgagacatgttagcagatcgcagtgccacctttcctgacgcgctcggccccatattcggacgcaattgtcatttgtagcacaattggagcaaatctggcgaggcagtaggcttttaagttgcaaggcgagagagcaaagtgggacgcggcgtgattattggtatttacgcgacggcccggcgcgttagcggcccttcccccaggccagggacgattatgtatcaatattgttgcgttcgggcactcgtgcgagggctcctgcgggctggggagggggatctgggaattggaggtacgaccgagatggcttgctcggggggaggtttcctcgccgagcaagccagggttaggtgttgcgctcttgactcgttgtgcattctaggaccccactgctactcacaacaagccctaactgtgccaaattatcttccaaactcctaatccaatt
BBa_K1884004_sequence
1
cggagtactgtcctccgagcggagtactgtcctccgactcgagcggagtactgtcctccgatcggagtactgtcctccgcgaatcccggagtactgtcctccgaagacgctagcggggggctataaaagggggtgggggcgttcgtcctcactctattagtattttaaatacctagata
BBa_K509003_sequence
1
ttctggcagcagctggaccgcctgtaccatggagaagagctttacttgccgggatggccgatttcgctgattgatacgggatcggagctcggaggctttcgcgctaggggctaggcgaagggcagtggtgaccagggtcggtgtggggtcggcccacggtcaattagccacaggaggatcagggggaggtaggcacgtcgacttggtttgcgaccccgcagttttggcggacgtgctgttgtagatgttagcgtgtgcgtgagccagtggccaacgtgccacacccattgagaagaccaaccaacttactggcaatatctgccaatgccatactgcatgtaatggccaggccatgtgagagtttgccgtgcctgcgcgcgccccgggggcgcagtttagctgaccagccgtgggatgatgcacgcatttgcaaggacagggtaatcacagcagcaacatggtgggcttaggacagctgtgggtcagtggacggacggcaggggagggacggcgcagctcgggagacaggggga
BBa_K1884008_sequence
1
taattcatcaaattataactatatagaccctaatttcatacacacacctgcccgtctgcctgacaggaagtgaacgcatgtcgagggaggcctcaccaatcgtcacacgagccctcgtcagaaacacgtctccgccacgctctccctctcacggccgaccccgcagcccttttgccctttcctaggccaccgacaggacccaggcgctctcagcatgcctcaacaacccgtactcgtgccagcggtgcccttgtgctggtgatcgcttggaagcgcatgcgaagacgaaggggcggagcaggcggcctggctgttcgaagggctcgccgccagttcgggtgcctttctccacgcgcgcctccacacctaccgatgcgtgaaggcaggcaaatgctcatgtttgcccgaactcggagtccttaaaaagccgcttcttgtcgtcgttccgagacatgttagcagatcgcagtgccacctttcctgacgcgctcggccccatattcggacgcaattgtcatttgtagcacaattggagcaaatctggcgaggcagtaggcttttaagttgcaaggcgagagagcaaagtgggacgcggcgtgattattggtatttacgcgacggcccggcgcgttagcggcccttcccccaggccagggacgattatgtatcaatattgttgcgttcgggcactcgtgcgagggctcctgcgggctggggagggggatctgggaattggaggtacgaccgagatggcttgctcggggggaggtttcctcgccgagcaagccagggttaggtgttgcgctcttgactcgttgtgcattctaggaccccactgctactcacaacaagccctaactgtgccaaattatcttccaaactcctaatccaatttactagatgggccccaagaagaagcgcaaggtggccccccccaccgacgtgagcctgggcgacgagctgcacctggacggcgaggacgtggcgatggcccacgccgacgccctggacgacttcgacctggacatgctgggcgacggcgacagccccggccccggcttcaccccccacgacagcgccccctacggcgccctggacatggccgacttcgagttcgagcagatgttcaccgacgccctgggcatcgacgagtacggcggccatatgatgaagatggacaaaaagactatagtttggtttagaagagacctaaggattgaggataatcctgcattagcagcagctgctcacgaaggatctgtttttcctgtcttcatttggtgtcctgaagaagaaggacagttttatcctggaagagcttcaagatggtggatgaaacaatcacttgctcacttatctcaatccttgaaggctcttggatctgacctcactttaatcaaaacccacaacacgatttcagcgatcttggattgtatccgcgttaccggtgctacaaaagtcgtctttaaccacctctatgatcctgtttcgttagttcgggaccataccgtaaaggagaagctggtggaacgtgggatctctgtgcaaagctacaatggagatctattgtatgaaccgtgggagatatactgcgaaaagggcaaaccttttacgagtttcaattcttactggaagaaatgcttagatatgtcgattgaatccgttatgcttcctcctccttggcggttgatgccaataactgcggcggctgaagcgatttgggcgtgttcgattgaagaactagggctggagaatgaggccgagaaaccgagcaatgcgttgttaactagagcttggtctccaggatggagcaatgctgataagttactaaatgagttcatcgagaagcagttgatagattatgcaaagaacagcaagaaagttgttgggaactctacttcactactttctccgtatctccatttcggggaaataagcgtcagacacgttttccagtgtgcccggatgaaacaaattatatgggcaagagataagaacagtgaaggagaagaaagtgcagatctttttcttaggggaatcggtttaagagagtattctcggtatatatgtttcaacttcccgtttactcacgagcaatcgttgttgagtcatcttcggtttttcccttgggatgctgatgttgataagttcaaggcctggagacaaggcaggaccggttatccgttggtggatgccggaatgagagagctttgggctaccggatggatgcataacagaataagagtgattgtttcaagctttgctgtgaagtttcttctccttccatggaaatggggaatgaagtatttctgggatacacttttggatgctgatttggaatgtgacatccttggctggcagtatatctctgggagtatccccgatggccacgagcttgatcgcttggacaatcccgcgttacaaggcgccaaatatgacccagaaggtgagtacataaggcaatggcttcccgagcttgcgagattgccaactgaatggatccatcatccatgggacgctcctttaaccgtactcaaagcttctggtgtggaactcggaacaaactatgcgaaacccattgtagacatcgacacagctcgtgagctactagctaaagctatttcaagaacccgtgaagcacagatcatgatcggagcagcacctgatgagattgtagcagatagcttcgaggccttaggggctaataccattaaagaacctggtctttgcccatctgtgtcttctaatgaccaacaagtaccttcggctgttcgttacaacgggtcaaagagagtgaaacctgaggaagaagaagagagagacatgaagaaatctaggggattcgatgaaagggagttgttttcgactgctgaatcttcttcttcttcgagtgtgtttttcgtttcgcagtcttgctcgttggcatcagaagggaagaatctggaaggtattcaagattcatctgatcagattactacaagtttgggaaaaaatggttgcaaatgatactagagcggagtactgtcctccgagcggagtactgtcctccgactcgagcggagtactgtcctccgatcggagtactgtcctccgcgaatcccggagtactgtcctccgaagacgctagcggggggctataaaagggggtgggggcgttcgtcctcactctattagtattttaaatacctagatatactagaggcggggccctgacaccactgcggccgcctactcggtggttcattaacgccgcgcctggacccgagggaggacccctcgggacccggtacgtcgttaatgaaccaccgaatacgcggtcgcggtggggtcaggtccttccgctactagagttctggcagcagctggaccgcctgtaccatggagaagagctttacttgccgggatggccgatttcgctgattgatacgggatcggagctcggaggctttcgcgctaggggctaggcgaagggcagtggtgaccagggtcggtgtggggtcggcccacggtcaattagccacaggaggatcagggggaggtaggcacgtcgacttggtttgcgaccccgcagttttggcggacgtgctgttgtagatgttagcgtgtgcgtgagccagtggccaacgtgccacacccattgagaagaccaaccaacttactggcaatatctgccaatgccatactgcatgtaatggccaggccatgtgagagtttgccgtgcctgcgcgcgccccgggggcgcagtttagctgaccagccgtgggatgatgcacgcatttgcaaggacagggtaatcacagcagcaacatggtgggcttaggacagctgtgggtcagtggacggacggcaggggagggacggcgcagctcgggagacaggggga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z