BBa_K1884005 1 D1 PsbA 2016-10-08T11:00:00Z 2016-10-19T05:06:35Z The DNA sequence of D1 protein is designed by ourselves. This family represents the D1 protein (also known as PsbA), which forms the reaction core of PSII as a heterodimer with the D2 protein. In higher plants, the N-terminal residues of both proteins, which are exposed to the stromal surface, can be reversibly phosphorylated. After insertion in the membrane, the C-terminal of the D1 protein is cleaved by a C-terminal processing protease to yield the mature protein . This processing is essential for the assembly of a functional 4-atom manganese cluster, which involves binding to a highly conserved C-terminal alanine 344. The Mn cluster is located on the lumenal surface of the D1 and D2 proteins. In addition to the Mn cluster, the D1/D2 core binds to a number of cofactors, including two pheophytin molecules, only one of which is phytochemically active; non-haem iron; and two quinones, Qa (bound to D2) and Qb (bound to D1). Upon light excitation, an electron is transferred from the primary donor (chlorophyll a) via intermediate acceptor pheophytin to the primary quinone Qa, then to the secondary quinone Qb. At the oxidising side of PSII, a redox-active residue in the D1 protein reduces P680, the oxidised tyrosine then withdrawing electrons from a manganese cluster, which in turn withdraw electrons from water, leading to the splitting of water and the formation of molecular oxygen. PSII thus provides a source of electrons that can be used by photosystem I to produce the reducing power (NADPH) required to convert CO2 to glucose. false false _2349_ 29275 29275 9 false false Changxing Hu BBa_K509003 1 BBa_K509003 PSAD Terminator 2011-09-18T11:00:00Z 2015-05-08T01:12:30Z plasmid PSAD Terminator false false _672_ 0 8421 9 Not in stock false to edit false Ben Jevans BBa_K1884004 1 Gal4-UAS Upstream activating sequence 2016-10-06T11:00:00Z 2016-10-15T07:42:46Z PhyB (photochrome B) PhyB is red/far-red photoreceptor involved in the regulation of de-etiolation. It is involved in the light-promotion of seed germination and in the shade avoidance response. false false _2349_ 29275 30234 9 No part sequence false PhyB functions as a transcriptional activator, and can be functionally and mechanistically separated from its role in repression of PIF3 mediated processes. false Chengrong Xie annotation2489054 1 UAS range2489054 1 1 179 BBa_K1884008 1 BBa_K1884008 PSAD Promoter+GalAD-CRY2+UAS+PsbA+PSAD Terminator 2016-10-10T11:00:00Z 2016-10-19T05:47:09Z false false _2349_ 29275 29275 9 false false Changxing Hu component2492798 1 BBa_K1884002 component2492800 1 BBa_K1884004 component2492797 1 BBa_K1547005 component2492802 1 BBa_K509003 component2492801 1 BBa_K1884005 annotation2492802 1 BBa_K509003 range2492802 1 3351 3883 annotation2492797 1 BBa_K1547005 range2492797 1 1 894 annotation2492800 1 BBa_K1884004 range2492800 1 3015 3193 annotation2492798 1 BBa_K1884002 range2492798 1 901 3006 annotation2492801 1 BBa_K1884005 range2492801 1 3202 3342 BBa_K1884002 1 AD-CRY2 VP16AD-CRY2 2016-10-06T11:00:00Z 2016-10-13T08:55:18Z Part cloned from pRMH120 of Addgene Cryptochrome 2 (CRY2) is a blue light stimulated photoreceptor, when exposed to blue light, it would interact with CIB1. This part is a Gal4 DNA activating domain fused to N terminus of CRY2. false false _2349_ 29275 30234 9 Not in stock false One powerful application of protein-interaction dimerizers(CRY2-CIB1) is to allow inducible control of a 'split' protein, in which two inactive fragments are brought together to reconstitute a functional protein activity. In principle, using this approach with optically controlled dimerizers would confer light-dependent activity to a diverse range of target proteins. We used the CRY2-CIB1 modules to reconstitute split versions of Gal4 transcription factor proteins. Based on the transient nature of the CRY-CIB interaction,the light pulses would allow precise, dose-dependent control of protein activity. We expressed in yeast the split Gal4 partners Gal4BD- CIB1 and Gal4AD-CRY2 together with upstream activating sequence under control of a galactose-inducible promoter. We incubated the yeast in the dark or subjected them to up to twenty blue-light pulses over 4 h. Immunoblot analysis of Snl1 revealed a strong dose dependence of protein expression in response to light. false Chengrong Xie BBa_K1547005 1 BBa_K1547005 PSAD Promoter 2014-10-07T11:00:00Z 2015-05-08T01:10:53Z Chlamydomonas reinhardtii nucleus This is a plant promoter from the green algae Chlamydomonas reinhardtii. false false _1931_ 0 22390 9 It's complicated false PSAD was amplified using PCR on a plasmid containing the promoter supplied by chlamycollection.org false victor yuan BBa_K1884002_sequence 1 atgggccccaagaagaagcgcaaggtggccccccccaccgacgtgagcctgggcgacgagctgcacctggacggcgaggacgtggcgatggcccacgccgacgccctggacgacttcgacctggacatgctgggcgacggcgacagccccggccccggcttcaccccccacgacagcgccccctacggcgccctggacatggccgacttcgagttcgagcagatgttcaccgacgccctgggcatcgacgagtacggcggccatatgatgaagatggacaaaaagactatagtttggtttagaagagacctaaggattgaggataatcctgcattagcagcagctgctcacgaaggatctgtttttcctgtcttcatttggtgtcctgaagaagaaggacagttttatcctggaagagcttcaagatggtggatgaaacaatcacttgctcacttatctcaatccttgaaggctcttggatctgacctcactttaatcaaaacccacaacacgatttcagcgatcttggattgtatccgcgttaccggtgctacaaaagtcgtctttaaccacctctatgatcctgtttcgttagttcgggaccataccgtaaaggagaagctggtggaacgtgggatctctgtgcaaagctacaatggagatctattgtatgaaccgtgggagatatactgcgaaaagggcaaaccttttacgagtttcaattcttactggaagaaatgcttagatatgtcgattgaatccgttatgcttcctcctccttggcggttgatgccaataactgcggcggctgaagcgatttgggcgtgttcgattgaagaactagggctggagaatgaggccgagaaaccgagcaatgcgttgttaactagagcttggtctccaggatggagcaatgctgataagttactaaatgagttcatcgagaagcagttgatagattatgcaaagaacagcaagaaagttgttgggaactctacttcactactttctccgtatctccatttcggggaaataagcgtcagacacgttttccagtgtgcccggatgaaacaaattatatgggcaagagataagaacagtgaaggagaagaaagtgcagatctttttcttaggggaatcggtttaagagagtattctcggtatatatgtttcaacttcccgtttactcacgagcaatcgttgttgagtcatcttcggtttttcccttgggatgctgatgttgataagttcaaggcctggagacaaggcaggaccggttatccgttggtggatgccggaatgagagagctttgggctaccggatggatgcataacagaataagagtgattgtttcaagctttgctgtgaagtttcttctccttccatggaaatggggaatgaagtatttctgggatacacttttggatgctgatttggaatgtgacatccttggctggcagtatatctctgggagtatccccgatggccacgagcttgatcgcttggacaatcccgcgttacaaggcgccaaatatgacccagaaggtgagtacataaggcaatggcttcccgagcttgcgagattgccaactgaatggatccatcatccatgggacgctcctttaaccgtactcaaagcttctggtgtggaactcggaacaaactatgcgaaacccattgtagacatcgacacagctcgtgagctactagctaaagctatttcaagaacccgtgaagcacagatcatgatcggagcagcacctgatgagattgtagcagatagcttcgaggccttaggggctaataccattaaagaacctggtctttgcccatctgtgtcttctaatgaccaacaagtaccttcggctgttcgttacaacgggtcaaagagagtgaaacctgaggaagaagaagagagagacatgaagaaatctaggggattcgatgaaagggagttgttttcgactgctgaatcttcttcttcttcgagtgtgtttttcgtttcgcagtcttgctcgttggcatcagaagggaagaatctggaaggtattcaagattcatctgatcagattactacaagtttgggaaaaaatggttgcaaatga BBa_K1884005_sequence 1 gcggggccctgacaccactgcggccgcctactcggtggttcattaacgccgcgcctggacccgagggaggacccctcgggacccggtacgtcgttaatgaaccaccgaatacgcggtcgcggtggggtcaggtccttccgc BBa_K1547005_sequence 1 taattcatcaaattataactatatagaccctaatttcatacacacacctgcccgtctgcctgacaggaagtgaacgcatgtcgagggaggcctcaccaatcgtcacacgagccctcgtcagaaacacgtctccgccacgctctccctctcacggccgaccccgcagcccttttgccctttcctaggccaccgacaggacccaggcgctctcagcatgcctcaacaacccgtactcgtgccagcggtgcccttgtgctggtgatcgcttggaagcgcatgcgaagacgaaggggcggagcaggcggcctggctgttcgaagggctcgccgccagttcgggtgcctttctccacgcgcgcctccacacctaccgatgcgtgaaggcaggcaaatgctcatgtttgcccgaactcggagtccttaaaaagccgcttcttgtcgtcgttccgagacatgttagcagatcgcagtgccacctttcctgacgcgctcggccccatattcggacgcaattgtcatttgtagcacaattggagcaaatctggcgaggcagtaggcttttaagttgcaaggcgagagagcaaagtgggacgcggcgtgattattggtatttacgcgacggcccggcgcgttagcggcccttcccccaggccagggacgattatgtatcaatattgttgcgttcgggcactcgtgcgagggctcctgcgggctggggagggggatctgggaattggaggtacgaccgagatggcttgctcggggggaggtttcctcgccgagcaagccagggttaggtgttgcgctcttgactcgttgtgcattctaggaccccactgctactcacaacaagccctaactgtgccaaattatcttccaaactcctaatccaatt BBa_K1884004_sequence 1 cggagtactgtcctccgagcggagtactgtcctccgactcgagcggagtactgtcctccgatcggagtactgtcctccgcgaatcccggagtactgtcctccgaagacgctagcggggggctataaaagggggtgggggcgttcgtcctcactctattagtattttaaatacctagata BBa_K509003_sequence 1 ttctggcagcagctggaccgcctgtaccatggagaagagctttacttgccgggatggccgatttcgctgattgatacgggatcggagctcggaggctttcgcgctaggggctaggcgaagggcagtggtgaccagggtcggtgtggggtcggcccacggtcaattagccacaggaggatcagggggaggtaggcacgtcgacttggtttgcgaccccgcagttttggcggacgtgctgttgtagatgttagcgtgtgcgtgagccagtggccaacgtgccacacccattgagaagaccaaccaacttactggcaatatctgccaatgccatactgcatgtaatggccaggccatgtgagagtttgccgtgcctgcgcgcgccccgggggcgcagtttagctgaccagccgtgggatgatgcacgcatttgcaaggacagggtaatcacagcagcaacatggtgggcttaggacagctgtgggtcagtggacggacggcaggggagggacggcgcagctcgggagacaggggga BBa_K1884008_sequence 1 taattcatcaaattataactatatagaccctaatttcatacacacacctgcccgtctgcctgacaggaagtgaacgcatgtcgagggaggcctcaccaatcgtcacacgagccctcgtcagaaacacgtctccgccacgctctccctctcacggccgaccccgcagcccttttgccctttcctaggccaccgacaggacccaggcgctctcagcatgcctcaacaacccgtactcgtgccagcggtgcccttgtgctggtgatcgcttggaagcgcatgcgaagacgaaggggcggagcaggcggcctggctgttcgaagggctcgccgccagttcgggtgcctttctccacgcgcgcctccacacctaccgatgcgtgaaggcaggcaaatgctcatgtttgcccgaactcggagtccttaaaaagccgcttcttgtcgtcgttccgagacatgttagcagatcgcagtgccacctttcctgacgcgctcggccccatattcggacgcaattgtcatttgtagcacaattggagcaaatctggcgaggcagtaggcttttaagttgcaaggcgagagagcaaagtgggacgcggcgtgattattggtatttacgcgacggcccggcgcgttagcggcccttcccccaggccagggacgattatgtatcaatattgttgcgttcgggcactcgtgcgagggctcctgcgggctggggagggggatctgggaattggaggtacgaccgagatggcttgctcggggggaggtttcctcgccgagcaagccagggttaggtgttgcgctcttgactcgttgtgcattctaggaccccactgctactcacaacaagccctaactgtgccaaattatcttccaaactcctaatccaatttactagatgggccccaagaagaagcgcaaggtggccccccccaccgacgtgagcctgggcgacgagctgcacctggacggcgaggacgtggcgatggcccacgccgacgccctggacgacttcgacctggacatgctgggcgacggcgacagccccggccccggcttcaccccccacgacagcgccccctacggcgccctggacatggccgacttcgagttcgagcagatgttcaccgacgccctgggcatcgacgagtacggcggccatatgatgaagatggacaaaaagactatagtttggtttagaagagacctaaggattgaggataatcctgcattagcagcagctgctcacgaaggatctgtttttcctgtcttcatttggtgtcctgaagaagaaggacagttttatcctggaagagcttcaagatggtggatgaaacaatcacttgctcacttatctcaatccttgaaggctcttggatctgacctcactttaatcaaaacccacaacacgatttcagcgatcttggattgtatccgcgttaccggtgctacaaaagtcgtctttaaccacctctatgatcctgtttcgttagttcgggaccataccgtaaaggagaagctggtggaacgtgggatctctgtgcaaagctacaatggagatctattgtatgaaccgtgggagatatactgcgaaaagggcaaaccttttacgagtttcaattcttactggaagaaatgcttagatatgtcgattgaatccgttatgcttcctcctccttggcggttgatgccaataactgcggcggctgaagcgatttgggcgtgttcgattgaagaactagggctggagaatgaggccgagaaaccgagcaatgcgttgttaactagagcttggtctccaggatggagcaatgctgataagttactaaatgagttcatcgagaagcagttgatagattatgcaaagaacagcaagaaagttgttgggaactctacttcactactttctccgtatctccatttcggggaaataagcgtcagacacgttttccagtgtgcccggatgaaacaaattatatgggcaagagataagaacagtgaaggagaagaaagtgcagatctttttcttaggggaatcggtttaagagagtattctcggtatatatgtttcaacttcccgtttactcacgagcaatcgttgttgagtcatcttcggtttttcccttgggatgctgatgttgataagttcaaggcctggagacaaggcaggaccggttatccgttggtggatgccggaatgagagagctttgggctaccggatggatgcataacagaataagagtgattgtttcaagctttgctgtgaagtttcttctccttccatggaaatggggaatgaagtatttctgggatacacttttggatgctgatttggaatgtgacatccttggctggcagtatatctctgggagtatccccgatggccacgagcttgatcgcttggacaatcccgcgttacaaggcgccaaatatgacccagaaggtgagtacataaggcaatggcttcccgagcttgcgagattgccaactgaatggatccatcatccatgggacgctcctttaaccgtactcaaagcttctggtgtggaactcggaacaaactatgcgaaacccattgtagacatcgacacagctcgtgagctactagctaaagctatttcaagaacccgtgaagcacagatcatgatcggagcagcacctgatgagattgtagcagatagcttcgaggccttaggggctaataccattaaagaacctggtctttgcccatctgtgtcttctaatgaccaacaagtaccttcggctgttcgttacaacgggtcaaagagagtgaaacctgaggaagaagaagagagagacatgaagaaatctaggggattcgatgaaagggagttgttttcgactgctgaatcttcttcttcttcgagtgtgtttttcgtttcgcagtcttgctcgttggcatcagaagggaagaatctggaaggtattcaagattcatctgatcagattactacaagtttgggaaaaaatggttgcaaatgatactagagcggagtactgtcctccgagcggagtactgtcctccgactcgagcggagtactgtcctccgatcggagtactgtcctccgcgaatcccggagtactgtcctccgaagacgctagcggggggctataaaagggggtgggggcgttcgtcctcactctattagtattttaaatacctagatatactagaggcggggccctgacaccactgcggccgcctactcggtggttcattaacgccgcgcctggacccgagggaggacccctcgggacccggtacgtcgttaatgaaccaccgaatacgcggtcgcggtggggtcaggtccttccgctactagagttctggcagcagctggaccgcctgtaccatggagaagagctttacttgccgggatggccgatttcgctgattgatacgggatcggagctcggaggctttcgcgctaggggctaggcgaagggcagtggtgaccagggtcggtgtggggtcggcccacggtcaattagccacaggaggatcagggggaggtaggcacgtcgacttggtttgcgaccccgcagttttggcggacgtgctgttgtagatgttagcgtgtgcgtgagccagtggccaacgtgccacacccattgagaagaccaaccaacttactggcaatatctgccaatgccatactgcatgtaatggccaggccatgtgagagtttgccgtgcctgcgcgcgccccgggggcgcagtttagctgaccagccgtgggatgatgcacgcatttgcaaggacagggtaatcacagcagcaacatggtgggcttaggacagctgtgggtcagtggacggacggcaggggagggacggcgcagctcgggagacaggggga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z